##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393942
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30541551802042	33.0	31.0	34.0	31.0	34.0
2	32.391202258200444	34.0	31.0	34.0	31.0	34.0
3	32.209708535774304	34.0	31.0	34.0	30.0	34.0
4	35.96932289524854	37.0	35.0	37.0	35.0	37.0
5	33.969581816612596	37.0	35.0	37.0	33.0	37.0
6	34.907636149483935	37.0	35.0	37.0	32.0	37.0
7	35.92903016180047	37.0	35.0	37.0	33.0	37.0
8	35.94321752948404	37.0	35.0	37.0	35.0	37.0
9	37.92538749359043	39.0	38.0	39.0	35.0	39.0
10	37.61969782353748	39.0	37.0	39.0	35.0	39.0
11	37.54858837087693	39.0	37.0	39.0	35.0	39.0
12	37.525950520635014	39.0	37.0	39.0	35.0	39.0
13	37.50200283290434	39.0	37.0	39.0	35.0	39.0
14	38.79110630498906	40.0	38.0	41.0	35.0	41.0
15	38.92885754755776	40.0	38.0	41.0	35.0	41.0
16	38.89912220580695	40.0	38.0	41.0	35.0	41.0
17	38.858131400053814	40.0	38.0	41.0	35.0	41.0
18	38.56140497839784	40.0	38.0	41.0	35.0	41.0
19	38.186859994618494	40.0	37.0	41.0	35.0	41.0
20	37.92761370963238	39.0	35.0	41.0	34.0	41.0
21	37.773636728249336	39.0	35.0	41.0	34.0	41.0
22	37.76570662686385	39.0	35.0	41.0	34.0	41.0
23	37.772481735890054	39.0	35.0	41.0	34.0	41.0
24	37.67669860030157	39.0	35.0	41.0	34.0	41.0
25	37.47943859756005	39.0	35.0	41.0	34.0	41.0
26	37.515730742088934	39.0	35.0	41.0	34.0	41.0
27	37.49945931126917	39.0	35.0	41.0	34.0	41.0
28	37.38819420117682	39.0	35.0	41.0	33.0	41.0
29	37.31655421356443	39.0	35.0	41.0	33.0	41.0
30	37.02687705296719	39.0	35.0	41.0	33.0	41.0
31	36.77471556726625	39.0	35.0	41.0	33.0	41.0
32	36.32824375161826	39.0	35.0	41.0	31.0	41.0
33	35.76859283853968	39.0	35.0	41.0	25.0	41.0
34	35.26751907641226	39.0	35.0	41.0	21.0	41.0
35	34.9335003629976	39.0	35.0	41.0	18.0	41.0
36	34.64958039508354	39.0	35.0	41.0	17.0	41.0
37	34.50393205091105	39.0	35.0	41.0	15.0	41.0
38	34.3608373821527	39.0	35.0	41.0	15.0	41.0
39	34.238253346939395	39.0	35.0	41.0	15.0	41.0
40	34.11710099456265	39.0	34.0	41.0	12.0	41.0
41	33.94778165313676	39.0	34.0	41.0	12.0	41.0
42	33.860712998360164	39.0	34.0	41.0	10.0	41.0
43	33.76359210239071	39.0	34.0	41.0	10.0	41.0
44	33.62597285894878	38.0	34.0	41.0	10.0	41.0
45	33.614224936665806	38.0	33.0	41.0	10.0	41.0
46	33.50195460245417	38.0	33.0	41.0	10.0	41.0
47	33.49195566860096	38.0	33.0	41.0	10.0	41.0
48	33.41778231313239	38.0	33.0	41.0	10.0	41.0
49	33.30418183387402	38.0	33.0	40.0	10.0	41.0
50	33.23446091048936	37.0	33.0	40.0	10.0	41.0
51	32.42109498352549	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	6.0
11	9.0
12	9.0
13	10.0
14	12.0
15	21.0
16	46.0
17	106.0
18	163.0
19	319.0
20	478.0
21	819.0
22	1242.0
23	2036.0
24	3427.0
25	6583.0
26	10642.0
27	12965.0
28	12053.0
29	10146.0
30	8828.0
31	8622.0
32	9172.0
33	11605.0
34	19671.0
35	27015.0
36	24243.0
37	33995.0
38	61368.0
39	128212.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.64403897020374	18.161556777393624	21.299582171994864	26.894822080407778
2	44.157261728883945	21.263282412131733	21.41000451843165	13.169451340552671
3	27.719308933802438	19.733361763914484	39.257047991836366	13.290281310446716
4	25.969051281660754	20.96323824319316	38.42773809342492	14.639972381721167
5	20.610394423544584	30.433160211401677	35.20873631143671	13.74770905361703
6	23.442283381817628	29.97674784612963	35.63824116240462	10.94272760964812
7	67.60995273415884	5.968898974976012	23.362830061278057	3.0583182295870968
8	68.17780282376593	6.127297927106021	23.158992948200495	2.5359063009275475
9	62.246980519975025	9.032040249579886	24.79121291966838	3.929766310776713
10	33.60646998796778	25.572800056861162	30.821034568540544	9.999695386630519
11	26.654939051941657	21.98064689725899	37.84364195744551	13.520772093353845
12	26.67626198780531	20.27785816186139	38.58207553396185	14.463804316371446
13	20.55302557229237	22.84295657736418	39.41722385528834	17.186793995055112
14	15.876448817338593	25.361093765072017	40.415086484812484	18.347370932776908
15	17.14516350122607	24.887419975529394	42.72938655944276	15.238029963801777
16	19.507947870498704	23.24606160297709	42.00288367323108	15.243106853293122
17	18.51744673073701	25.145833650638927	39.64695310477177	16.68976651385229
18	18.800737164354146	25.594122992724817	39.848505617578226	15.756634225342816
19	21.30948210650299	24.041610186271075	39.303755375156754	15.345152332069187
20	22.094369221865147	23.221692533418626	40.811337709612076	13.872600535104151
21	21.094221992069897	24.534576155880817	39.339547446070746	15.031654405978545
22	20.254758314675765	22.714257428758547	40.250087576343724	16.780896680221964
23	20.717262947337424	23.320184189550748	40.97455970675886	14.987993156352966
24	18.480639281924752	23.483406186697533	42.011514385366375	16.024440146011344
25	18.739052957034282	25.307532580938314	39.94369729554097	16.009717166486436
26	17.946042818485967	27.042305720131388	39.018434185743075	15.993217275639562
27	18.328332597184357	26.842783963121477	40.23028770732747	14.598595732366693
28	17.519076412263736	26.356925638799623	40.8951063862193	15.228891562717353
29	17.598275888328736	25.035918993151274	40.38589437023724	16.97991074828274
30	17.12155596509131	26.308187499682695	39.40580085393281	17.164455681293187
31	19.253595706982246	26.039620045590468	37.77891161643085	16.92787263099644
32	19.22567281477984	26.427240558254766	37.77891161643085	16.568175010534546
33	18.534200466058458	27.770839362139604	36.55563509349092	17.13932507831102
34	18.261063811424016	27.331942265612703	36.83638708236238	17.5706068406009
35	20.692640033304396	26.017789420777675	34.88000771687202	18.4095628290459
36	19.486878779109613	29.817841205050488	34.01998263703794	16.675297378801957
37	19.817891973945404	29.689142056444854	34.76171619172366	15.731249777886086
38	18.736006823339476	28.765402013494374	34.892699940600394	17.60589122256576
39	20.15575896959451	28.003107056368705	34.175081610998575	17.666052363038215
40	20.465703073041208	28.27675139995228	34.77136228175721	16.4861832452493
41	17.99427326865376	29.318529123576564	34.94829188053063	17.738905727239036
42	18.533438932634752	29.729249483426496	32.91271303897528	18.82459854496347
43	20.158805103289318	29.08321529565266	32.632722583527524	18.1252570175305
44	19.256387996202488	29.693711256987072	32.46239294109285	18.587507805717593
45	19.024120301973387	29.356351950287095	32.17199486218784	19.44753288555168
46	18.35727086728503	31.10584806900508	32.460616029770875	18.076265033939006
47	20.36061146057034	27.122520574094665	35.19731331008118	17.319554655253818
48	19.21628056922085	27.731493468581668	35.06810647252641	17.98411948967107
49	18.25801767772921	29.174599306496894	34.40252626021089	18.164856755563
50	17.64092176005605	27.798508409867445	35.64560265216707	18.91496717790944
51	19.21704210264455	26.9948368033873	33.451117169532566	20.337003924435574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	1000.0
2	1655.0
3	13081.5
4	24508.0
5	16680.0
6	8852.0
7	8159.5
8	7467.0
9	7030.5
10	6594.0
11	6283.0
12	5972.0
13	5671.0
14	5370.0
15	4986.5
16	4603.0
17	4369.5
18	4136.0
19	3762.5
20	3389.0
21	3353.0
22	3317.0
23	3433.0
24	3549.0
25	3878.0
26	5151.0
27	6095.0
28	7661.0
29	9227.0
30	10498.5
31	11770.0
32	12367.5
33	12965.0
34	13659.0
35	14353.0
36	14873.5
37	15394.0
38	15702.5
39	16011.0
40	17689.0
41	19367.0
42	20363.0
43	21359.0
44	21915.5
45	22472.0
46	22532.5
47	22593.0
48	21386.5
49	20180.0
50	19494.5
51	18809.0
52	17871.5
53	16934.0
54	15309.5
55	13685.0
56	13224.5
57	12764.0
58	12916.0
59	13068.0
60	12886.0
61	12704.0
62	11739.5
63	10775.0
64	8919.5
65	7064.0
66	5858.0
67	4652.0
68	3966.0
69	3280.0
70	2536.0
71	1792.0
72	1482.0
73	1172.0
74	936.5
75	558.0
76	415.0
77	305.0
78	195.0
79	130.5
80	66.0
81	59.5
82	53.0
83	40.5
84	28.0
85	22.5
86	17.0
87	13.0
88	9.0
89	6.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	393942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.69760058507953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8599153521854	23.2871738189933
2	6.779551293530937	4.16231915509744
3	2.8109315100166987	2.5886655829952128
4	1.7096259847724888	2.099256625216765
5	1.2635260328097828	1.9393608742022406
6	1.065271763135029	1.9620772259569546
7	0.9123007351721101	1.9603810507251498
8	0.785162379481184	1.9282080895795222
9	0.7477706844354474	2.0659289220027817
>10	7.820841926699135	46.886077335285
>50	0.19358930406270866	3.6936727188700433
>100	0.04486619064114278	2.6956516341484247
>500	0.004154276911216924	0.8454995900465538
>1k	0.0016617107644867697	0.52081754536201
>5k	0.0	0.0
>10k+	8.308553822433849E-4	3.364909831518608
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13193	3.3489701529666807	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1035	0.2627290311771784	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1007	0.25562138588929334	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	826	0.20967553599260802	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.18479877748501047	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	602	0.1528143736895279	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	581	0.14748363972361414	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	578	0.14672210629991217	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	427	0.10839159064024655	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	412	0.1045839235217367	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	405	0.10280701219976544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.538444745673221E-4	0.0	0.0	0.02538444745673221	0.0
2	2.538444745673221E-4	0.0	0.0	0.1307299044021709	0.0
3	2.538444745673221E-4	0.0	0.0	0.18149879931563528	0.0
4	2.538444745673221E-4	0.0	0.0	0.23480613897477293	0.0
5	2.538444745673221E-4	0.0	0.0	0.4124972711718984	0.0
6	2.538444745673221E-4	0.0	0.0	0.5127658386259907	0.0
7	2.538444745673221E-4	0.0	0.0	0.6112574947581116	0.0
8	2.538444745673221E-4	0.0	0.0	0.7343720649232628	0.0
9	2.538444745673221E-4	0.0	0.0	0.7831102040401886	0.0
10	2.538444745673221E-4	0.0	0.0	0.9245015763741871	0.0
11	2.538444745673221E-4	0.0	0.0	1.185961385178529	0.0
12	2.538444745673221E-4	0.0	0.0	1.380660097171665	0.0
13	2.538444745673221E-4	0.0	0.0	1.4547826837453228	0.0
14	2.538444745673221E-4	0.0	0.0	1.481690198049459	0.0
15	5.076889491346442E-4	0.0	0.0	1.539820582725376	0.0
16	5.076889491346442E-4	0.0	0.0	1.6733427763477873	0.0
17	5.076889491346442E-4	0.0	0.0	1.8167649044783243	0.0
18	5.076889491346442E-4	0.0	0.0	1.9477486533550623	0.0
19	5.076889491346442E-4	0.0	0.0	2.048271065283722	0.0
20	5.076889491346442E-4	0.0	0.0	2.1678318128049305	0.0
21	5.076889491346442E-4	0.0	0.0	2.308969340664362	0.0
22	5.076889491346442E-4	0.0	0.0	2.4559452914388413	0.0
23	5.076889491346442E-4	0.0	0.0	2.592005929806926	0.0
24	5.076889491346442E-4	0.0	0.0	2.715882033395779	0.0
25	5.076889491346442E-4	0.0	0.0	2.826304379832564	0.0
26	5.076889491346442E-4	0.0	0.0	2.928603703083195	0.0
27	5.076889491346442E-4	0.0	0.0	3.0336953155540662	0.0
28	5.076889491346442E-4	0.0	0.0	3.138279239075803	0.0
29	5.076889491346442E-4	0.0	0.0	3.264186098461195	0.0
30	5.076889491346442E-4	0.0	0.0	3.3870468241517786	0.0
31	5.076889491346442E-4	0.0	0.0	3.5218382401470265	0.0
32	5.076889491346442E-4	0.0	0.0	3.6454604992613127	0.0
33	7.615334237019663E-4	0.0	0.0	3.76324433546055	0.0
34	7.615334237019663E-4	0.0	0.0	3.8817897050834893	0.0
35	7.615334237019663E-4	0.0	0.0	4.017850343451574	0.0
36	7.615334237019663E-4	0.0	0.0	4.152895603921389	0.0
37	7.615334237019663E-4	0.0	0.0	4.295302354153657	0.0
38	7.615334237019663E-4	0.0	0.0	4.425778414081261	0.0
39	7.615334237019663E-4	0.0	0.0	4.56133136350021	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1618544E-6	45.000004	6
GCACGTC	20	7.027634E-4	45.0	39
CGCGCGG	35	1.2092096E-7	45.0	2
GCGTTAG	20	7.027634E-4	45.0	1
ACGGGCT	45	3.8380676E-10	45.0	5
GCACCAC	45	3.8380676E-10	45.0	29
CGTTAGG	20	7.027634E-4	45.0	2
TTGCGCG	20	7.027634E-4	45.0	1
CGGGAAT	20	7.027634E-4	45.0	6
GCGATAT	40	6.7957444E-9	45.0	9
ACGTCCC	20	7.027634E-4	45.0	43
AACACGG	20	7.027634E-4	45.0	2
CGTTATT	520	0.0	44.567307	1
CGTTTTT	6780	0.0	43.34071	1
ACATACG	270	0.0	41.666664	17
CTACGAA	65	0.0	41.53846	11
GCTACGA	65	0.0	41.53846	10
CCATCGA	55	6.002665E-11	40.909092	31
CATACGA	270	0.0	40.833332	18
TTGAGCG	50	1.0768417E-9	40.5	1
>>END_MODULE