FastQCFastQC Report
Sat 18 Jun 2016
SRR3550874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190776
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136917.176479221705036No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.42825093303140854No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6600.34595546609636435No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3950.20704910470918775No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC2680.14047888623306914No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2260.11846353839057323No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA2250.11793936344194238No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA2240.11741518849331153No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2070.10850421436658698No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2040.10693168952069443No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA2030.10640751457206357No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAG207.018482E-445.00000439
ATGGGCG207.018482E-445.0000045
AGATCAG207.018482E-445.00000444
GATCAGG207.018482E-445.0000042
ATCACGG207.018482E-445.0000042
CGGGGCT207.018482E-445.0000046
ATATCAT207.018482E-445.00000426
CACTATC207.018482E-445.00000436
AATTGCA207.018482E-445.00000441
GGAGTTC406.7630026E-945.0000048
CGGGAGT406.7630026E-945.0000046
TATAGCG207.018482E-445.0000041
TGGGCTC207.018482E-445.0000046
AGCTATG207.018482E-445.0000041
AATACGG207.018482E-445.0000042
GGGATAC207.018482E-445.0000047
CGAAGGA207.018482E-445.0000042
CTCACTA253.87854E-545.045
GGGTCAT253.87854E-545.07
GGCAATG351.2048076E-745.01