##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	190776
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17936742567199	33.0	31.0	34.0	30.0	34.0
2	32.32121440852099	34.0	31.0	34.0	30.0	34.0
3	31.926248584727638	33.0	31.0	34.0	30.0	34.0
4	35.8498081519688	37.0	35.0	37.0	35.0	37.0
5	33.91960728812849	37.0	35.0	37.0	32.0	37.0
6	34.87435526481318	37.0	35.0	37.0	32.0	37.0
7	35.88668385960498	37.0	35.0	37.0	33.0	37.0
8	35.91280349729526	37.0	35.0	37.0	35.0	37.0
9	37.92765861533945	39.0	38.0	39.0	35.0	39.0
10	37.70926636474189	39.0	38.0	39.0	35.0	39.0
11	37.64263848702143	39.0	37.0	39.0	35.0	39.0
12	37.56736696439804	39.0	37.0	39.0	35.0	39.0
13	37.520993206692665	39.0	37.0	39.0	35.0	39.0
14	38.83382081603556	40.0	38.0	41.0	35.0	41.0
15	38.97906445255168	41.0	38.0	41.0	35.0	41.0
16	38.93092422526943	40.0	38.0	41.0	35.0	41.0
17	38.87620560238185	40.0	38.0	41.0	35.0	41.0
18	38.33976495995304	39.0	38.0	41.0	35.0	41.0
19	37.72638591856418	38.0	37.0	41.0	35.0	41.0
20	37.12524112047637	38.0	35.0	41.0	34.0	41.0
21	36.96016794565354	37.0	35.0	40.0	33.0	41.0
22	36.949789281670654	37.0	35.0	41.0	33.0	41.0
23	36.96612257306999	37.0	35.0	41.0	33.0	41.0
24	36.81663836121944	37.0	35.0	41.0	33.0	41.0
25	36.60514425294586	36.0	35.0	40.0	33.0	41.0
26	36.64462511007674	36.0	35.0	40.0	33.0	41.0
27	36.606842579779425	36.0	35.0	40.0	33.0	41.0
28	36.42438776366	36.0	35.0	40.0	33.0	41.0
29	36.28251981381306	36.0	35.0	40.0	33.0	41.0
30	35.854562418752884	36.0	35.0	40.0	32.0	41.0
31	35.337448106680085	36.0	35.0	40.0	30.0	41.0
32	34.54138361219441	35.0	35.0	40.0	21.0	41.0
33	33.52232461106219	35.0	33.0	40.0	15.0	41.0
34	32.65262926154233	35.0	33.0	40.0	10.0	41.0
35	32.021035140688554	35.0	31.0	40.0	8.0	41.0
36	31.638848702142827	35.0	30.0	40.0	7.0	41.0
37	31.42821948253449	35.0	29.0	40.0	7.0	41.0
38	31.233650983352202	35.0	26.0	40.0	7.0	41.0
39	31.06334654254204	35.0	25.0	40.0	7.0	41.0
40	30.92052459428859	35.0	24.0	40.0	7.0	41.0
41	30.71186103073762	35.0	23.0	40.0	7.0	41.0
42	30.58282488363316	35.0	23.0	40.0	7.0	41.0
43	30.425137333836542	35.0	23.0	40.0	7.0	41.0
44	30.250571350694006	35.0	22.0	40.0	7.0	41.0
45	30.22546337065459	35.0	23.0	40.0	7.0	41.0
46	30.142460267538894	35.0	23.0	40.0	7.0	41.0
47	30.112571811967964	35.0	23.0	40.0	7.0	41.0
48	30.0487377867237	35.0	23.0	40.0	7.0	41.0
49	29.95170776198264	35.0	22.0	40.0	7.0	41.0
50	29.872185180525854	35.0	22.0	40.0	7.0	41.0
51	29.207311192183504	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	5.0
12	2.0
13	7.0
14	7.0
15	22.0
16	39.0
17	54.0
18	120.0
19	236.0
20	420.0
21	617.0
22	983.0
23	1607.0
24	2955.0
25	5332.0
26	8703.0
27	10508.0
28	9527.0
29	7602.0
30	6094.0
31	5284.0
32	5087.0
33	6141.0
34	9731.0
35	15740.0
36	12510.0
37	13570.0
38	23648.0
39	44194.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.305468193064115	15.924959114354007	17.315071078123033	42.45450161445884
2	54.34645867404705	18.052585230846645	16.83387847527991	10.767077619826393
3	21.82926573573196	16.419256090912903	50.77577892397367	10.975699249381474
4	20.566528284480228	17.569820103157628	50.00891097412672	11.854740638235416
5	16.726422610810584	25.496393676353417	46.70975384744413	11.067429865391873
6	18.162661970059126	24.729001551557847	48.170629429278314	8.93770704910471
7	53.19012873736738	5.152639745041305	39.097685243426845	2.559546274164465
8	54.31920157671824	4.647859269509792	38.50274667673082	2.5301924770411373
9	48.96685117624858	7.323772382270306	39.90596301421562	3.8034134272654843
10	27.322095022434688	20.501530590850003	44.28020296054011	7.8961714261752
11	22.642261081058415	17.445066465383483	48.95794020212186	10.954732251436239
12	21.252149117289388	15.729441858514697	51.87235291650941	11.146056107686501
13	15.434331362435527	19.499308089067807	51.86344194238269	13.202918606113975
14	11.80075061852644	21.48226191973833	52.40281796452384	14.31416949721139
15	12.59277896590766	19.52499266155072	55.56621377951105	12.31601459303057
16	14.536943850379503	17.695622090829037	55.386945947079305	12.380488111712165
17	13.40105673669644	18.461965865727343	52.99932905606575	15.137648341510463
18	14.311024447519605	19.77659663689353	53.236256132846904	12.676122782739968
19	17.09072420010903	19.067912106344615	51.37543506520736	12.465928628338995
20	17.898477795949177	18.828888329768944	51.88807816496834	11.38455570931354
21	15.664444164884472	19.681720971191343	51.77275967626955	12.88107518765463
22	15.662871640038578	19.167505346584477	51.589298444248755	13.58032456912819
23	15.518199354216463	19.87409317733887	51.51905900113222	13.08864846731245
24	13.82983184467648	19.20052836834822	53.773535455193525	13.196104331781774
25	13.786849498888747	20.864783830251184	52.14649641464335	13.201870256216717
26	13.574558644693251	21.78733173984149	51.14689478760431	13.491214827860947
27	15.112487943976182	20.914056275422485	51.60449951775905	12.368956262842286
28	13.054052920702814	21.432989474567034	52.872478718497085	12.640478886233069
29	13.360171090703233	20.795592737031914	51.99238897974588	13.851847192518976
30	13.41311276051495	22.359206608797752	50.16511510881871	14.062565521868578
31	16.029269929131548	21.649473728351577	48.47517507443284	13.846081268084035
32	16.37994296976559	22.283725416194912	47.49706462028767	13.839266993751833
33	15.81802742483331	24.603199563886445	45.5303602130247	14.048412798255546
34	15.722627584182497	24.960162703904054	44.03121985994045	15.285989851972994
35	17.139996645280327	23.946932528200612	41.85117624858473	17.061894577934332
36	17.180358116324904	27.04847569924938	40.21941963349688	15.551746550928838
37	16.56445255168365	28.37621084413134	40.81540655009016	14.243930054094855
38	16.11156539606659	28.25355390615172	39.485574705413676	16.149305992368014
39	17.307208453893573	27.079402021218602	40.17853398750367	15.434855537384157
40	17.42252694259236	27.006017528410283	40.114584643770705	15.456870885226653
41	14.634964565773473	27.47200905774311	40.21941963349688	17.67360674298654
42	16.345871598104583	27.68063068729819	37.935065207363614	18.038432507233615
43	17.994925986497254	27.538055101270597	37.38310898645532	17.083909925776826
44	16.993227659663688	27.682203212144085	37.58439216672957	17.74017696146266
45	16.100557722145343	29.20178638822493	37.589633916215874	17.108021973413848
46	16.814484002180567	28.252505556254455	38.04094854698704	16.892061894577935
47	17.338134775862795	26.222900155155788	40.17381641296599	16.265148656015434
48	15.915523965278652	25.39837296095945	41.16398289092968	17.52212018283222
49	15.648718916425548	26.54998532310144	40.753553906151716	17.0477418543213
50	15.07474734767476	25.94403908248417	41.10160607204261	17.879607497798467
51	15.580576173103536	24.8726254874827	39.618190967417284	19.928607371996478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	264.0
1	573.5
2	883.0
3	11804.5
4	22726.0
5	14873.0
6	7020.0
7	6653.5
8	6287.0
9	6083.5
10	5880.0
11	5613.0
12	5346.0
13	5028.0
14	4710.0
15	4327.5
16	3945.0
17	3653.0
18	3361.0
19	3045.0
20	2729.0
21	2530.0
22	2331.0
23	2260.5
24	2190.0
25	2350.5
26	2592.5
27	2674.0
28	3238.5
29	3803.0
30	3679.0
31	3555.0
32	4469.0
33	5383.0
34	5612.5
35	5842.0
36	5809.0
37	5776.0
38	5944.0
39	6112.0
40	6388.5
41	6665.0
42	7654.0
43	8643.0
44	8833.5
45	9024.0
46	9154.5
47	9285.0
48	9093.0
49	8901.0
50	8625.5
51	8350.0
52	7397.5
53	6445.0
54	5813.5
55	5182.0
56	4892.5
57	4603.0
58	4558.5
59	4514.0
60	4666.5
61	4819.0
62	4494.0
63	4169.0
64	3422.0
65	2675.0
66	2065.0
67	1455.0
68	1149.5
69	844.0
70	782.5
71	721.0
72	559.5
73	398.0
74	381.5
75	264.5
76	164.0
77	139.0
78	114.0
79	96.0
80	78.0
81	45.0
82	12.0
83	15.0
84	18.0
85	9.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	190776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.77072587746886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34909651654739	32.353126179393634
2	6.655859133024923	5.294166981171636
3	2.452782939912749	2.9264687382060637
4	1.2428663688004957	1.9771879062355853
5	0.9819039711096174	1.9525516836499348
6	0.7934311283328721	1.8933199144546484
7	0.6800838242853189	1.8933199144546484
8	0.5785984474055329	1.840902419591563
9	0.5298327468269345	1.8964649641464337
>10	4.554980032422601	34.19245607413931
>50	0.14234312601320628	3.5318908038747017
>100	0.03426778959577188	2.2974587998490374
>500	0.0026359838150593756	0.7742063991277729
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0013179919075296878	7.176479221705036
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13691	7.176479221705036	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.42825093303140854	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	660	0.34595546609636435	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	395	0.20704910470918775	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC	268	0.14047888623306914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	226	0.11846353839057323	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	225	0.11793936344194238	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	224	0.11741518849331153	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	207	0.10850421436658698	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	204	0.10693168952069443	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	203	0.10640751457206357	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00104834989726171	0.0	0.0	0.037216421352790706	0.0
2	0.00104834989726171	0.0	0.0	0.245838050907871	0.0
3	0.00104834989726171	0.0	0.0	0.35958401476076657	0.0
4	0.00104834989726171	0.0	0.0	0.49534532645615803	0.0
5	0.00104834989726171	0.0	0.0	0.8554535161655554	0.0
6	0.00104834989726171	0.0	0.0	1.037866398289093	0.0
7	0.00104834989726171	0.0	0.0	1.2517297773304819	0.0
8	0.00104834989726171	0.0	0.0	1.536880949385667	0.0
9	0.00104834989726171	0.0	0.0	1.6459093387008847	0.0
10	0.00104834989726171	0.0	0.0	1.9074726380676814	0.0
11	0.00104834989726171	0.0	0.0	2.457856334130079	0.0
12	0.00104834989726171	0.0	0.0	2.877196293034763	0.0
13	0.00104834989726171	0.0	0.0	3.0019499308089066	0.0
14	0.00104834989726171	0.0	0.0	3.054891600620623	0.0
15	0.00104834989726171	0.0	0.0	3.1476705665282845	0.0
16	0.00104834989726171	0.0	0.0	3.3500020966997943	0.0
17	0.00104834989726171	0.0	0.0	3.567534700381599	0.0
18	0.00104834989726171	0.0	0.0	3.833815574286074	0.0
19	0.00104834989726171	0.0	0.0	4.006269132385625	0.0
20	0.00104834989726171	0.0	0.0	4.157755692539942	0.0
21	0.001572524845892565	0.0	0.0	4.3506520736360965	0.0
22	0.001572524845892565	0.0	0.0	4.57342642680421	0.0
23	0.001572524845892565	0.0	0.0	4.788862330691492	0.0
24	0.001572524845892565	0.0	0.0	4.971275212815029	0.0
25	0.001572524845892565	0.0	0.0	5.120140898226192	0.0
26	0.001572524845892565	0.0	0.0	5.261668134356523	0.0
27	0.001572524845892565	0.0	0.0	5.400050320795069	0.0
28	0.001572524845892565	0.0	0.0	5.576697278483667	0.0
29	0.001572524845892565	0.0	0.0	5.744433262045541	0.0
30	0.001572524845892565	0.0	0.0	5.938377993038957	0.0
31	0.001572524845892565	0.0	0.0	6.122363400008386	0.0
32	0.001572524845892565	0.0	0.0	6.305824632029186	0.0
33	0.001572524845892565	0.0	0.0	6.477229840231476	0.0
34	0.001572524845892565	0.0	0.0	6.655449322765967	0.0
35	0.001572524845892565	0.0	0.0	6.856208328091584	0.0
36	0.001572524845892565	0.0	0.0	7.058015683314463	0.0
37	0.001572524845892565	0.0	0.0	7.238856040592108	0.0
38	0.001572524845892565	0.0	0.0	7.422841447561538	0.0
39	0.001572524845892565	0.0	0.0	7.615737828657693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAAG	20	7.018482E-4	45.000004	39
ATGGGCG	20	7.018482E-4	45.000004	5
AGATCAG	20	7.018482E-4	45.000004	44
GATCAGG	20	7.018482E-4	45.000004	2
ATCACGG	20	7.018482E-4	45.000004	2
CGGGGCT	20	7.018482E-4	45.000004	6
ATATCAT	20	7.018482E-4	45.000004	26
CACTATC	20	7.018482E-4	45.000004	36
AATTGCA	20	7.018482E-4	45.000004	41
GGAGTTC	40	6.7630026E-9	45.000004	8
CGGGAGT	40	6.7630026E-9	45.000004	6
TATAGCG	20	7.018482E-4	45.000004	1
TGGGCTC	20	7.018482E-4	45.000004	6
AGCTATG	20	7.018482E-4	45.000004	1
AATACGG	20	7.018482E-4	45.000004	2
GGGATAC	20	7.018482E-4	45.000004	7
CGAAGGA	20	7.018482E-4	45.000004	2
CTCACTA	25	3.87854E-5	45.0	45
GGGTCAT	25	3.87854E-5	45.0	7
GGCAATG	35	1.2048076E-7	45.0	1
>>END_MODULE