FastQCFastQC Report
Sat 18 Jun 2016
SRR3550871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430829
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105972.4596765770177957No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11640.2701767986834684No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9160.2126133570395679No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8700.20193626705723153No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7190.1668875586369534No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7140.1657270053780038No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1439086041097512No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA4670.10839567438589325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG207.02837E-445.00000445
GGATGCG207.02837E-445.00000440
CCGATGC207.02837E-445.00000411
GCGCGAC207.02837E-445.0000049
TAGCGAG406.7975634E-945.0000041
CGCGACC207.02837E-445.00000410
AATAGCG207.02837E-445.0000041
TATACGG406.7975634E-945.0000042
AAATGCG207.02837E-445.0000041
ACAACGG253.886732E-545.02
TTGTGCG453.8380676E-1045.01
CGGGCTA351.2095552E-745.06
GTATACG351.2095552E-745.01
GCTACGA900.045.010
ATAAGCG302.162331E-644.9999961
CGTAAGG302.162331E-644.9999962
CGTTATT3850.043.8311651
CGTTTTT56700.043.2936481
TACGAAT900.042.512
GCACGGG1450.041.896553