##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430829
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.258125613642534	33.0	31.0	34.0	30.0	34.0
2	32.33564592912733	34.0	31.0	34.0	31.0	34.0
3	32.20702413254447	34.0	31.0	34.0	30.0	34.0
4	35.94235996184101	37.0	35.0	37.0	35.0	37.0
5	33.90181951539938	37.0	35.0	37.0	32.0	37.0
6	34.84949481116638	37.0	35.0	37.0	32.0	37.0
7	35.868460572524135	37.0	35.0	37.0	33.0	37.0
8	35.89789452427761	37.0	35.0	37.0	35.0	37.0
9	37.85238226767465	39.0	38.0	39.0	35.0	39.0
10	37.503464251477965	39.0	37.0	39.0	35.0	39.0
11	37.43072541542004	39.0	37.0	39.0	35.0	39.0
12	37.41759027363525	39.0	37.0	39.0	35.0	39.0
13	37.40038158991154	39.0	37.0	39.0	35.0	39.0
14	38.66794946486889	40.0	38.0	41.0	35.0	41.0
15	38.811096746040775	40.0	38.0	41.0	35.0	41.0
16	38.756546100657104	40.0	38.0	41.0	35.0	41.0
17	38.72723052533604	40.0	38.0	41.0	35.0	41.0
18	38.498459945825374	40.0	38.0	41.0	35.0	41.0
19	38.207954896258144	40.0	37.0	41.0	35.0	41.0
20	37.98229459948146	40.0	35.0	41.0	34.0	41.0
21	37.855418275000055	40.0	35.0	41.0	34.0	41.0
22	37.84277520779706	40.0	35.0	41.0	34.0	41.0
23	37.85244493755063	40.0	35.0	41.0	34.0	41.0
24	37.76415933003582	40.0	35.0	41.0	34.0	41.0
25	37.55875068762781	39.0	35.0	41.0	33.0	41.0
26	37.59001135021087	39.0	35.0	41.0	34.0	41.0
27	37.58542948594454	40.0	35.0	41.0	34.0	41.0
28	37.46241548270892	39.0	35.0	41.0	33.0	41.0
29	37.40713600987863	40.0	35.0	41.0	33.0	41.0
30	37.14767343888178	39.0	35.0	41.0	33.0	41.0
31	36.94330929440683	39.0	35.0	41.0	33.0	41.0
32	36.588551374211114	39.0	35.0	41.0	31.0	41.0
33	36.14498327642754	39.0	35.0	41.0	29.0	41.0
34	35.734063862924735	40.0	35.0	41.0	24.0	41.0
35	35.451557346418184	39.0	35.0	41.0	22.0	41.0
36	35.23301124111887	39.0	35.0	41.0	21.0	41.0
37	35.10916860285636	39.0	35.0	41.0	20.0	41.0
38	34.97123220581716	39.0	35.0	41.0	18.0	41.0
39	34.86837469158297	39.0	35.0	41.0	18.0	41.0
40	34.77695559026899	39.0	35.0	41.0	18.0	41.0
41	34.633265170171924	39.0	35.0	41.0	18.0	41.0
42	34.561183207258566	39.0	35.0	41.0	17.0	41.0
43	34.47491928352084	39.0	35.0	41.0	17.0	41.0
44	34.30889981872158	39.0	34.0	41.0	15.0	41.0
45	34.288829210661305	38.0	34.0	41.0	17.0	41.0
46	34.19452033173254	38.0	34.0	41.0	17.0	41.0
47	34.18726455275759	38.0	34.0	41.0	17.0	41.0
48	34.11760582504892	38.0	34.0	41.0	17.0	41.0
49	34.031488131021824	38.0	34.0	41.0	17.0	41.0
50	33.96073616214322	38.0	34.0	40.0	17.0	41.0
51	33.04723915985228	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	6.0
10	12.0
11	6.0
12	10.0
13	16.0
14	15.0
15	26.0
16	52.0
17	108.0
18	198.0
19	346.0
20	588.0
21	897.0
22	1336.0
23	2171.0
24	3581.0
25	6139.0
26	9681.0
27	11907.0
28	11250.0
29	9653.0
30	8726.0
31	8873.0
32	9873.0
33	12847.0
34	22208.0
35	29818.0
36	27571.0
37	39585.0
38	71610.0
39	141599.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.3806266523377	18.735739701830656	22.159603926383788	23.724029719447856
2	40.60590164543241	22.49593226082738	22.243627982331738	14.654538111408472
3	28.794022686495108	20.977928598121295	35.962063835071454	14.265984880312143
4	26.258445926342006	22.432333942236944	35.14364167686019	16.16557845456086
5	22.042388047229874	31.570298192554354	31.590259708608286	14.797054051607484
6	24.73719271451086	31.24627172265562	31.917303616980284	12.099231945853226
7	70.5818317708418	6.396505342026651	19.655594214874114	3.3660686722574384
8	72.41573803063397	5.979634611411952	19.01961102896973	2.5850163289843535
9	65.99787850864264	8.735020158810109	20.678970078615876	4.588131253931374
10	36.818552140176266	25.401493399933617	26.896053886808918	10.883900573081199
11	27.937766492042087	23.362633434610945	33.99701505701798	14.702585016328984
12	26.818528929111086	21.951632782380017	35.52035726471524	15.709481023793662
13	22.30235197723459	22.723400699581507	37.124473979235376	17.849773343948527
14	17.761339185616567	25.762425463466943	37.02118473918887	19.45505061172762
15	18.836707835359274	25.668420649492024	38.66684926037941	16.828022254769294
16	20.87904946045879	24.341444053209045	38.37137240065084	16.408134085681326
17	19.979852795424634	25.4755366978546	37.28416610766685	17.260444399053917
18	20.047164884443713	25.92838457949674	36.50357798569735	17.52087255036221
19	22.594347177186307	24.662917305938088	35.73970183065671	17.00303368621889
20	23.043249177748017	24.409916695487073	37.90668687576741	14.640147250997495
21	23.184836675339866	24.91916746551416	36.17351663885207	15.722479220293897
22	21.863894956003428	23.608670725508265	37.10474457383324	17.422689744655074
23	22.231790339090452	24.21169419885848	37.08988949211868	16.466625969932387
24	20.17366518966922	24.408291920924544	38.0013880216977	17.416654867708534
25	19.824106548073598	26.659068911331406	36.417232823231494	17.099591717363502
26	19.29141260221573	27.186656422849904	36.378702455034365	17.143228519900006
27	20.240280946732927	26.4037471943625	37.27627434550599	16.07969751339859
28	18.60274029835503	26.53419338066843	37.696858846549326	17.16620747442721
29	19.528629688345028	25.46044950548826	37.13747217573562	17.8734486304311
30	19.396790838128354	26.260999143511693	36.470618273143174	17.87159174521678
31	20.794793293859048	26.57829440450852	34.52065668745604	18.10625561417639
32	20.46310717245125	27.53807194965984	34.67988459458393	17.31893628330498
33	19.919736136611046	28.399434578452237	33.85078534639033	17.830043938546382
34	19.628205157962906	28.10395771872367	34.258835872237015	18.009001251076413
35	22.08532851781101	26.67856620608176	32.1380408468325	19.098064429274725
36	20.423416250995174	29.716198306056462	31.296871844745827	18.563513598202537
37	20.61119376829322	30.221503195003123	32.11715088817141	17.05015214853225
38	20.31664535117181	29.46760779798946	32.085583839527985	18.130163011310753
39	21.395727771343154	27.87347184149628	32.07421041759027	18.656589969570295
40	21.14110238632961	28.640365435010178	32.32164037239833	17.89689180626188
41	18.823245417555455	29.029847108713664	32.94137581267742	19.205531661053456
42	19.443445079138126	29.27402751439666	31.287587418674228	19.99493998779098
43	21.134139066775916	28.54357529321378	31.090757585956375	19.23152805405393
44	20.3950987514768	29.669079843743106	30.513266284303054	19.422555120477032
45	20.162059657079723	29.125012475947532	30.49028732977585	20.22264053719689
46	19.327621863894954	30.53322780035699	30.752340255646672	19.386810080101384
47	20.806863047752124	27.955406901578122	32.6563439322794	18.58138611839036
48	19.718031980205602	27.53760772835626	33.5567011505725	19.187659140865634
49	19.127310371400256	28.998744281373817	32.58601440478705	19.28793094243888
50	18.392680158485152	28.885474283300333	33.453411910525986	19.268433647688525
51	19.28955571700141	27.709601721332593	32.41123508398924	20.589607477676758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	296.0
1	1008.0
2	1720.0
3	11178.0
4	20636.0
5	14101.0
6	7566.0
7	7069.5
8	6573.0
9	6186.5
10	5800.0
11	5585.0
12	5370.0
13	5093.0
14	4816.0
15	4373.0
16	3930.0
17	3639.0
18	3348.0
19	3174.0
20	3000.0
21	2941.5
22	2883.0
23	3019.5
24	3156.0
25	3575.5
26	4833.0
27	5671.0
28	7027.5
29	8384.0
30	10124.5
31	11865.0
32	12556.0
33	13247.0
34	14727.5
35	16208.0
36	16267.5
37	16327.0
38	17410.5
39	18494.0
40	20010.5
41	21527.0
42	22434.0
43	23341.0
44	24334.5
45	25328.0
46	25298.5
47	25269.0
48	24966.0
49	24663.0
50	23725.0
51	22787.0
52	21697.0
53	20607.0
54	19221.5
55	17836.0
56	17238.0
57	16640.0
58	16297.0
59	15954.0
60	15647.5
61	15341.0
62	13836.5
63	12332.0
64	10758.5
65	9185.0
66	7737.0
67	6289.0
68	5251.5
69	4214.0
70	3398.0
71	2582.0
72	2034.5
73	1487.0
74	1266.5
75	805.5
76	565.0
77	412.0
78	259.0
79	216.0
80	173.0
81	119.0
82	65.0
83	53.5
84	42.0
85	24.0
86	6.0
87	5.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.970867996728273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21776248931144	21.943998945829556
2	7.073917006674361	4.240228656536969
3	3.1801014270861643	2.8593120026221985
4	2.0454052201394965	2.4521027941047917
5	1.603660405812845	2.4031547167098233
6	1.316411393546366	2.3672395263220345
7	1.0941750697087604	2.2955363605686547
8	0.9945002132555822	2.3844827691360937
9	0.8692785635658066	2.344772977291472
>10	8.41196394190964	47.87948237968911
>50	0.14073061567382317	2.5814914706667813
>100	0.04665103282005188	2.6129503394644
>500	0.003887586068337657	0.8945970171411602
>1k	7.775172136675313E-4	0.2712453576327978
>5k	0.0	0.0
>10k+	7.775172136675313E-4	2.469404686284156
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10597	2.4596765770177957	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1164	0.2701767986834684	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	916	0.2126133570395679	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	870	0.20193626705723153	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	719	0.1668875586369534	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	714	0.1657270053780038	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	620	0.1439086041097512	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	467	0.10839567438589325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.642213035798426E-4	0.0	0.0	0.012766085848445671	0.0
2	4.642213035798426E-4	0.0	0.0	0.08518460920690112	0.0
3	4.642213035798426E-4	0.0	0.0	0.1144305513324312	0.0
4	4.642213035798426E-4	0.0	0.0	0.1569068006099868	0.0
5	4.642213035798426E-4	0.0	0.0	0.3117246053538643	0.0
6	4.642213035798426E-4	0.0	0.0	0.39226700152496696	0.0
7	4.642213035798426E-4	0.0	0.0	0.477683721383658	0.0
8	4.642213035798426E-4	0.0	0.0	0.5988454816179969	0.0
9	4.642213035798426E-4	0.0	0.0	0.6366795178597541	0.0
10	4.642213035798426E-4	0.0	0.0	0.7963716462912199	0.0
11	4.642213035798426E-4	0.0	0.0	0.9976115813930817	0.0
12	4.642213035798426E-4	0.0	0.0	1.1638028080746654	0.0
13	4.642213035798426E-4	0.0	0.0	1.2164919260309774	0.0
14	4.642213035798426E-4	0.0	0.0	1.2373818846920703	0.0
15	4.642213035798426E-4	0.0	0.0	1.2821792404875252	0.0
16	4.642213035798426E-4	0.0	0.0	1.373862947944544	0.0
17	4.642213035798426E-4	0.0	0.0	1.4815622903750676	0.0
18	4.642213035798426E-4	0.0	0.0	1.5964570630110786	0.0
19	4.642213035798426E-4	0.0	0.0	1.6841948893876688	0.0
20	4.642213035798426E-4	0.0	0.0	1.760327183174763	0.0
21	6.963319553697639E-4	0.0	0.0	1.8684907469088663	0.0
22	6.963319553697639E-4	0.0	0.0	1.9745653147768605	0.0
23	6.963319553697639E-4	0.0	0.0	2.087603202198552	0.0
24	6.963319553697639E-4	0.0	0.0	2.1839291226913695	0.0
25	6.963319553697639E-4	0.0	0.0	2.281647707094926	0.0
26	6.963319553697639E-4	0.0	0.0	2.355691005015911	0.0
27	6.963319553697639E-4	0.0	0.0	2.441804056829972	0.0
28	9.284426071596852E-4	0.0	0.0	2.527452887340453	0.0
29	9.284426071596852E-4	0.0	0.0	2.62517147174401	0.0
30	9.284426071596852E-4	0.0	0.0	2.7291570437458947	0.0
31	9.284426071596852E-4	0.0	0.0	2.8319820624888297	0.0
32	9.284426071596852E-4	0.0	0.0	2.9529117120713786	0.0
33	9.284426071596852E-4	0.0	0.0	3.054576177555364	0.0
34	9.284426071596852E-4	0.0	0.0	3.1680782862806356	0.0
35	0.0011605532589496065	0.0	0.0	3.282973058916647	0.0
36	0.0011605532589496065	0.0	0.0	3.4050632617581456	0.0
37	0.0011605532589496065	0.0	0.0	3.518333259831627	0.0
38	0.0011605532589496065	0.0	0.0	3.6376381348516467	0.0
39	0.0011605532589496065	0.0	0.0	3.749051247710809	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCG	20	7.02837E-4	45.000004	45
GGATGCG	20	7.02837E-4	45.000004	40
CCGATGC	20	7.02837E-4	45.000004	11
GCGCGAC	20	7.02837E-4	45.000004	9
TAGCGAG	40	6.7975634E-9	45.000004	1
CGCGACC	20	7.02837E-4	45.000004	10
AATAGCG	20	7.02837E-4	45.000004	1
TATACGG	40	6.7975634E-9	45.000004	2
AAATGCG	20	7.02837E-4	45.000004	1
ACAACGG	25	3.886732E-5	45.0	2
TTGTGCG	45	3.8380676E-10	45.0	1
CGGGCTA	35	1.2095552E-7	45.0	6
GTATACG	35	1.2095552E-7	45.0	1
GCTACGA	90	0.0	45.0	10
ATAAGCG	30	2.162331E-6	44.999996	1
CGTAAGG	30	2.162331E-6	44.999996	2
CGTTATT	385	0.0	43.831165	1
CGTTTTT	5670	0.0	43.293648	1
TACGAAT	90	0.0	42.5	12
GCACGGG	145	0.0	41.89655	3
>>END_MODULE