FastQCFastQC Report
Sat 18 Jun 2016
SRR3550870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740292
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186472.518870932010612No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC57920.7823939742696125No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG52050.7031009385485727No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC29100.39308813279084465No Hit
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT19570.2643551463476574No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC11460.15480378013000276TruSeq Adapter, Index 22 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.13724314189536022No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCT9340.12616643162427799No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9010.12170873114933027No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8550.11549496685091828No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8370.11306349386458316No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8060.10887595705478378No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG207.0316566E-445.00000432
CGTAAGG800.045.0000042
TACGTAG207.0316566E-445.0000041
TAGTACG207.0316566E-445.0000041
CGAATAC253.8894555E-545.045
GTATACG253.8894555E-545.01
GTACCGG302.16445E-644.9999962
CGTTATT7850.044.4267541
CGTTTTT105300.043.6538471
CGATGAA11650.043.64806719
CCGATGA11550.042.6623418
ACCACCG14950.042.59197214
CCACCGA14600.041.30137315
GATGAAT13150.041.0646420
CTACGAA1100.040.90909211
GCTACGA1100.040.90909210
CGACCAC18850.040.22546412
TGATACC6850.040.0729944
TTGTGCG451.9266736E-840.01
TACGGCT3950.039.8734177