FastQCFastQC Report
Sat 18 Jun 2016
SRR3550869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576725
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152382.64216047509645No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG45570.7901512852746109No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC43230.7495773548918462TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC25810.4475269842645975No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC13650.2366812605661277TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC12000.20807143786033205TruSeq Adapter, Index 16 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT10110.17530018639732975No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.16541679309896398No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT7780.1348996488794486TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC6860.11894750531015648No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5880.1019550045515627No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT5830.10108804022714464TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.030359E-445.0000041
GTAGGTG253.8883794E-545.00000435
CGATTGA253.8883794E-545.00000410
ATATGCG302.1636133E-645.0000041
CGACAAT207.030359E-445.00000420
TCGTGTA253.8883794E-545.00000417
TACGCCC302.1636133E-645.00000424
AATTGCG302.1636133E-645.0000041
GCGATAT351.2105193E-745.0000049
TTAACGG502.1827873E-1145.0000042
ATGCGAG302.1636133E-645.0000041
CGCACGG207.030359E-445.0000042
TCGACAA207.030359E-445.00000419
GATTACG253.8883794E-545.0000041
CAATACG351.2105193E-745.00000421
GCGTAAG302.1636133E-645.0000041
TCAACGG207.030359E-445.0000042
TACCGTA207.030359E-445.00000423
CGTTTTT83500.043.679641
TACGGCT3550.041.1971867
Produced by FastQC (version 0.11.5)