##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	576725
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.082706662620836	33.0	31.0	34.0	30.0	34.0
2	32.17050067189735	33.0	31.0	34.0	30.0	34.0
3	32.035431098010314	33.0	31.0	34.0	30.0	34.0
4	35.80674498244397	37.0	35.0	37.0	35.0	37.0
5	33.81623824179635	37.0	35.0	37.0	32.0	37.0
6	34.773044345225195	37.0	35.0	37.0	32.0	37.0
7	35.82643721010881	37.0	35.0	37.0	33.0	37.0
8	35.815203086393	37.0	35.0	37.0	35.0	37.0
9	37.783685465343105	39.0	38.0	39.0	35.0	39.0
10	37.43842212492956	39.0	37.0	39.0	34.0	39.0
11	37.36842689323334	39.0	37.0	39.0	34.0	39.0
12	37.422617365295416	39.0	37.0	39.0	35.0	39.0
13	37.421216350947155	39.0	37.0	39.0	35.0	39.0
14	38.68296501798951	40.0	38.0	41.0	35.0	41.0
15	38.76261823225974	40.0	38.0	41.0	35.0	41.0
16	38.76780441284841	40.0	38.0	41.0	35.0	41.0
17	38.707467163726214	40.0	38.0	41.0	35.0	41.0
18	38.42322944210846	40.0	38.0	41.0	35.0	41.0
19	38.14731804586241	40.0	37.0	41.0	34.0	41.0
20	37.87851749100525	39.0	35.0	41.0	34.0	41.0
21	37.78906757986909	39.0	35.0	41.0	34.0	41.0
22	37.77952923837184	39.0	35.0	41.0	34.0	41.0
23	37.753322640773334	39.0	35.0	41.0	34.0	41.0
24	37.63913476960423	39.0	35.0	41.0	34.0	41.0
25	37.412456543413235	39.0	35.0	41.0	33.0	41.0
26	37.35440287832156	39.0	35.0	41.0	33.0	41.0
27	37.35156790498071	39.0	35.0	41.0	33.0	41.0
28	37.13432745242533	39.0	35.0	41.0	33.0	41.0
29	37.133313104165765	39.0	35.0	41.0	33.0	41.0
30	36.81621396679527	39.0	35.0	41.0	32.0	41.0
31	36.55323594434089	39.0	35.0	41.0	31.0	41.0
32	36.16787897178031	39.0	35.0	41.0	30.0	41.0
33	35.53779357579435	39.0	35.0	41.0	24.0	41.0
34	35.21403441848368	39.0	35.0	41.0	21.0	41.0
35	34.91510685335299	39.0	35.0	41.0	18.0	41.0
36	34.74912306558585	39.0	35.0	41.0	18.0	41.0
37	34.67958212319563	39.0	35.0	41.0	18.0	41.0
38	34.53669773288829	39.0	35.0	41.0	16.0	41.0
39	34.465731501148724	39.0	35.0	41.0	15.0	41.0
40	34.34370280462959	39.0	34.0	41.0	15.0	41.0
41	34.2069339806667	39.0	34.0	41.0	15.0	41.0
42	34.170313407603274	39.0	34.0	41.0	15.0	41.0
43	34.09016082188218	39.0	34.0	41.0	15.0	41.0
44	33.935610559625474	39.0	34.0	41.0	12.0	41.0
45	33.91162165676882	38.0	34.0	41.0	13.0	41.0
46	33.83042177814383	38.0	33.0	41.0	12.0	41.0
47	33.797922753478694	38.0	33.0	41.0	12.0	41.0
48	33.74260175993758	38.0	33.0	40.0	12.0	41.0
49	33.70618405652608	38.0	33.0	40.0	12.0	41.0
50	33.63365555507391	38.0	33.0	40.0	12.0	41.0
51	32.618365772248474	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	10.0
11	8.0
12	9.0
13	11.0
14	14.0
15	24.0
16	44.0
17	100.0
18	215.0
19	405.0
20	726.0
21	1268.0
22	1976.0
23	3265.0
24	5390.0
25	9186.0
26	14603.0
27	17180.0
28	16214.0
29	14228.0
30	13024.0
31	13697.0
32	15025.0
33	18670.0
34	28064.0
35	38190.0
36	38561.0
37	56084.0
38	99788.0
39	170613.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.856560752525034	17.530712211192508	20.786163249382287	24.82656378690017
2	39.34890979236204	24.112185183579697	22.442932073345183	14.09597295071308
3	26.068230092331703	23.305561576141145	37.47418613724045	13.152022194286705
4	23.603970696605835	20.627508778013784	40.643807707312845	15.124712818067538
5	21.260739520568727	29.816290259655815	33.90680133512506	15.016168884650396
6	22.501712254540728	30.483506003727946	35.439594260696175	11.575187481035156
7	68.10871732628202	4.619012527634488	23.72239802332134	3.549872122762148
8	67.81134856300663	6.588408686982531	22.002167410811044	3.598075339199792
9	62.75157137283801	7.194936928345398	22.688456370020376	7.365035328796219
10	31.59928908925398	29.42789024231653	28.96978629329403	10.003034375135462
11	22.715505656942216	21.233516840782002	40.33239412198188	15.718583380293902
12	21.483549351944166	19.342494256361352	42.3168754605748	16.857080931119683
13	19.381160865230395	20.6051410984438	43.868048029823576	16.145650006502233
14	15.168581212883089	25.188261303047383	40.78616324938229	18.856994234687242
15	14.726776193159651	24.04612250205904	45.21010880402272	16.016992500758594
16	17.02544540292167	23.880705708960075	41.324201309116134	17.769647579002125
17	16.95192682821102	23.27192336035372	40.80818414322251	18.967965668212752
18	16.184143222506396	24.63929949282587	41.8693484763102	17.307208808357537
19	18.250639386189256	23.594087303307468	39.75655641770341	18.39871689279986
20	19.998266071351164	24.344531622523732	41.25172309159478	14.405479214530322
21	19.258051931163035	24.291820191599115	40.88395682517664	15.56617105206121
22	17.563310069790628	21.828774545927434	40.396549481988814	20.21136590229312
23	17.448870778967446	23.77424248992154	41.76340543586631	17.013481295244702
24	17.791321687112575	22.51818457670467	41.09202826303698	18.59846547314578
25	16.3141878711691	26.886471021717455	39.15020156920543	17.649139537908017
26	16.478564307078763	24.959556114265897	40.70102735272443	17.860852225930905
27	18.0904243790368	24.840261823225973	39.82105856344012	17.24825523429711
28	14.808617625384715	24.835753608739	42.23867527851229	18.116953487363997
29	16.992500758593785	22.09267848628029	42.20555724131952	18.709263513806405
30	16.3988035892323	23.970523212969784	40.59941913390264	19.03125406389527
31	16.91430057653128	23.527851228921932	39.63830248385279	19.919545710694006
32	18.188391347696044	24.825350036845983	38.17556027569465	18.810698339763317
33	16.62490788504053	24.591963240712644	38.02436169751615	20.75876717673068
34	16.72478217521349	24.085656075252505	38.547314578005114	20.64224717152889
35	17.071221119250943	23.529585157570764	37.2430534483506	22.156140274827692
36	17.3150114872773	25.529671854003205	36.29476787030214	20.860548788417358
37	17.002210759027268	26.136200095366075	36.08305518227925	20.77853396332741
38	16.521218951840133	26.365945641336857	35.6698599852616	21.442975421561403
39	18.611816723741818	25.42459577788374	34.48680046816073	21.476787030213707
40	18.00719580389267	25.212536304131085	37.62104989379687	19.159217998179376
41	15.974164463132343	26.862889592093286	34.55858511422255	22.604360830551823
42	17.456500065022322	26.175040097100005	35.65217391304348	20.716285924834192
43	18.778620659759852	25.63197364428454	33.86223936884997	21.72716632710564
44	18.198101348129526	25.689019896831244	34.32069010360224	21.792188651436994
45	17.375005418527028	25.268542199488493	34.524600112705365	22.831852269279118
46	18.730764229051974	26.62724868871646	33.673241146126834	20.968745936104728
47	16.71125753175257	25.22276648315922	37.74675972083749	20.319216264250723
48	18.056786163249384	24.48948805756643	35.04841995751875	22.40530582166544
49	16.26112965451472	24.56855520395336	37.503316138540896	21.666999002991027
50	16.69790628115653	24.925224327018945	35.93255017556027	22.44431921626425
51	17.15861112315228	23.8116953487364	34.17677402574884	24.85291950236248
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	785.5
2	1327.0
3	15243.5
4	29160.0
5	19805.5
6	10451.0
7	9875.5
8	9300.0
9	8901.0
10	8502.0
11	8180.0
12	7858.0
13	7401.0
14	6944.0
15	6349.5
16	5755.0
17	5483.5
18	5212.0
19	4997.0
20	4782.0
21	4900.0
22	5018.0
23	5173.0
24	5328.0
25	5577.0
26	6701.5
27	7577.0
28	9048.0
29	10519.0
30	13196.5
31	15874.0
32	16196.0
33	16518.0
34	17796.0
35	19074.0
36	21016.0
37	22958.0
38	23713.0
39	24468.0
40	26838.5
41	29209.0
42	31326.0
43	33443.0
44	35816.0
45	38189.0
46	47482.0
47	56775.0
48	50688.5
49	44602.0
50	41838.5
51	39075.0
52	33366.0
53	27657.0
54	23539.0
55	19421.0
56	17277.5
57	15134.0
58	14719.0
59	14304.0
60	13333.5
61	12363.0
62	10789.5
63	9216.0
64	7448.5
65	5681.0
66	4471.5
67	3262.0
68	2794.0
69	2326.0
70	1841.0
71	1356.0
72	1123.5
73	891.0
74	710.0
75	411.0
76	293.0
77	227.5
78	162.0
79	113.0
80	64.0
81	56.0
82	48.0
83	34.0
84	20.0
85	12.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	576725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.165276949712094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15803533086944	26.361256459748255
2	8.644815971384915	5.907050636833199
3	3.1548325238098114	3.233571807177641
4	1.5940426243560406	2.1784363092308
5	1.0453139023577889	1.7856719486719081
6	0.805179463423527	1.6505507617251225
7	0.6192190181044585	1.4809052472247326
8	0.5392083446357485	1.4737761942460923
9	0.4729044803536671	1.4541221287838448
>10	5.701794611091619	41.41763863487839
>50	0.2207997397349406	4.578998922986671
>100	0.037224898384864855	2.484189234214222
>500	0.003059580689166974	0.7193503996122114
>1k	0.003059580689166974	2.6197316442162317
>5k	0.0	0.0
>10k+	5.099301148611624E-4	2.6547496704506846
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15238	2.64216047509645	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	4557	0.7901512852746109	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	4323	0.7495773548918462	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	2581	0.4475269842645975	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1365	0.2366812605661277	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1200	0.20807143786033205	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	1011	0.17530018639732975	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	954	0.16541679309896398	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	778	0.1348996488794486	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC	686	0.11894750531015648	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	588	0.1019550045515627	No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	583	0.10108804022714464	TruSeq Adapter, Index 27 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.467857297672201E-4	0.0	0.0	0.10750357622783822	0.0
2	5.201785946508301E-4	0.0	0.0	0.7214877107807014	0.0
3	5.201785946508301E-4	0.0	0.0	0.991113615674715	0.0
4	5.201785946508301E-4	0.0	0.0	1.410377562963284	0.0
5	5.201785946508301E-4	0.0	0.0	3.073388530061988	0.0
6	5.201785946508301E-4	0.0	0.0	3.555420694438424	0.0
7	5.201785946508301E-4	0.0	0.0	4.221249295591487	0.0
8	5.201785946508301E-4	0.0	0.0	4.990246651350297	0.0
9	5.201785946508301E-4	0.0	0.0	5.223980233213403	0.0
10	5.201785946508301E-4	0.0	0.0	6.725215657375699	0.0
11	5.201785946508301E-4	0.0	0.0	7.601543196497464	0.0
12	5.201785946508301E-4	0.0	0.0	8.846850752091552	0.0
13	6.935714595344402E-4	0.0	0.0	9.158784516017166	0.0
14	6.935714595344402E-4	0.0	0.0	9.286921843166153	0.0
15	6.935714595344402E-4	0.0	0.0	9.59122632103689	0.0
16	6.935714595344402E-4	0.0	0.0	10.033724912219862	0.0
17	6.935714595344402E-4	0.0	0.0	10.589449044171833	0.0
18	6.935714595344402E-4	0.0	0.0	11.127140318175908	0.0
19	6.935714595344402E-4	0.0	0.0	11.609345875417226	0.0
20	6.935714595344402E-4	0.0	0.0	11.98751571372838	0.0
21	6.935714595344402E-4	0.0	0.0	12.436429840912046	0.0
22	6.935714595344402E-4	0.0	0.0	13.022844509948415	0.0
23	6.935714595344402E-4	0.0	0.0	13.513719710433916	0.0
24	6.935714595344402E-4	0.0	0.0	13.914257228315055	0.0
25	6.935714595344402E-4	0.0	0.0	14.242316528674845	0.0
26	8.669643244180502E-4	0.0	0.0	14.53552386319303	0.0
27	8.669643244180502E-4	0.0	0.0	14.874853699770254	0.0
28	0.0010403571893016603	0.0	0.0	15.196670856994235	0.0
29	0.0010403571893016603	0.0	0.0	15.546924444059126	0.0
30	0.0010403571893016603	0.0	0.0	15.95197017642724	0.0
31	0.0010403571893016603	0.0	0.0	16.32805930035979	0.0
32	0.0010403571893016603	0.0	0.0	16.69634574537258	0.0
33	0.0010403571893016603	0.0	0.0	17.02059040270493	0.0
34	0.0010403571893016603	0.0	0.0	17.347956131605184	0.0
35	0.0010403571893016603	0.0	0.0	17.691794182669383	0.0
36	0.0010403571893016603	0.0	0.0	18.076206164116346	0.0
37	0.0010403571893016603	0.0	0.0	18.42212492955915	0.0
38	0.0010403571893016603	0.0	0.0	18.764402444839394	0.0
39	0.0010403571893016603	0.0	0.0	19.081884780441285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.030359E-4	45.000004	1
GTAGGTG	25	3.8883794E-5	45.000004	35
CGATTGA	25	3.8883794E-5	45.000004	10
ATATGCG	30	2.1636133E-6	45.000004	1
CGACAAT	20	7.030359E-4	45.000004	20
TCGTGTA	25	3.8883794E-5	45.000004	17
TACGCCC	30	2.1636133E-6	45.000004	24
AATTGCG	30	2.1636133E-6	45.000004	1
GCGATAT	35	1.2105193E-7	45.000004	9
TTAACGG	50	2.1827873E-11	45.000004	2
ATGCGAG	30	2.1636133E-6	45.000004	1
CGCACGG	20	7.030359E-4	45.000004	2
TCGACAA	20	7.030359E-4	45.000004	19
GATTACG	25	3.8883794E-5	45.000004	1
CAATACG	35	1.2105193E-7	45.000004	21
GCGTAAG	30	2.1636133E-6	45.000004	1
TCAACGG	20	7.030359E-4	45.000004	2
TACCGTA	20	7.030359E-4	45.000004	23
CGTTTTT	8350	0.0	43.67964	1
TACGGCT	355	0.0	41.197186	7
>>END_MODULE