##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262973
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14476391112395	33.0	31.0	34.0	30.0	34.0
2	32.28104406155765	34.0	31.0	34.0	30.0	34.0
3	31.915036904929405	33.0	31.0	34.0	30.0	34.0
4	35.84058059192389	37.0	35.0	37.0	35.0	37.0
5	33.761534454107455	37.0	35.0	37.0	32.0	37.0
6	34.784282036558885	37.0	35.0	37.0	32.0	37.0
7	35.872983157966786	37.0	35.0	37.0	33.0	37.0
8	35.89942313469444	37.0	35.0	37.0	35.0	37.0
9	37.91866845645751	39.0	38.0	39.0	35.0	39.0
10	37.67441524415054	39.0	37.0	39.0	35.0	39.0
11	37.57972111205333	39.0	37.0	39.0	35.0	39.0
12	37.54489244142935	39.0	37.0	39.0	35.0	39.0
13	37.506352363170365	39.0	37.0	39.0	35.0	39.0
14	38.77313260296685	40.0	38.0	41.0	35.0	41.0
15	38.90194050339769	40.0	38.0	41.0	35.0	41.0
16	38.866377917124574	40.0	38.0	41.0	35.0	41.0
17	38.82324040871116	40.0	38.0	41.0	35.0	41.0
18	38.32893490966753	39.0	38.0	41.0	35.0	41.0
19	37.73091534111867	38.0	37.0	41.0	34.0	41.0
20	37.220231734816885	38.0	35.0	41.0	34.0	41.0
21	37.0642727580398	37.0	35.0	41.0	33.0	41.0
22	37.05771695193042	38.0	35.0	41.0	34.0	41.0
23	37.06852034239257	38.0	35.0	41.0	34.0	41.0
24	36.938358690816166	37.0	35.0	41.0	33.0	41.0
25	36.73129180562263	37.0	35.0	40.0	33.0	41.0
26	36.76926528578979	37.0	35.0	40.0	33.0	41.0
27	36.73978697432816	37.0	35.0	41.0	33.0	41.0
28	36.55364238914261	37.0	35.0	40.0	33.0	41.0
29	36.42445802420781	37.0	35.0	41.0	33.0	41.0
30	36.01177307176022	37.0	35.0	40.0	32.0	41.0
31	35.522821734550696	37.0	35.0	40.0	30.0	41.0
32	34.74718697356763	37.0	35.0	40.0	22.0	41.0
33	33.76968357968308	37.0	34.0	40.0	16.0	41.0
34	32.92695828088815	36.0	33.0	40.0	12.0	41.0
35	32.391139014271424	36.0	32.0	40.0	10.0	41.0
36	32.0376502530678	36.0	31.0	40.0	8.0	41.0
37	31.833686348028124	36.0	30.0	40.0	7.0	41.0
38	31.669414730789853	36.0	30.0	40.0	7.0	41.0
39	31.530784529210223	36.0	29.0	40.0	7.0	41.0
40	31.416343122677993	36.0	28.0	40.0	7.0	41.0
41	31.24181189703886	36.0	26.0	40.0	7.0	41.0
42	31.097960627136626	35.0	25.0	40.0	7.0	41.0
43	30.918105661037444	35.0	24.0	40.0	7.0	41.0
44	30.732501055241414	35.0	23.0	40.0	7.0	41.0
45	30.714073307906133	35.0	23.0	40.0	7.0	41.0
46	30.603784418932744	35.0	23.0	40.0	7.0	41.0
47	30.575903229609125	35.0	23.0	40.0	7.0	41.0
48	30.5185323208086	35.0	23.0	40.0	7.0	41.0
49	30.400607666946797	35.0	23.0	40.0	7.0	41.0
50	30.29715217912105	35.0	23.0	40.0	7.0	41.0
51	29.581291615489043	35.0	22.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	5.0
11	7.0
12	8.0
13	8.0
14	5.0
15	25.0
16	33.0
17	72.0
18	154.0
19	326.0
20	558.0
21	803.0
22	1311.0
23	2376.0
24	4144.0
25	7532.0
26	11708.0
27	13091.0
28	11561.0
29	9401.0
30	7882.0
31	7102.0
32	7058.0
33	8401.0
34	13569.0
35	21220.0
36	17218.0
37	20043.0
38	35185.0
39	62104.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.908085620957284	15.890224471713827	18.501519167366993	38.7001707399619
2	52.31753830241127	17.788137945720663	18.083985808429002	11.81033794343906
3	21.3139751989748	17.08540420499443	49.799409064808934	11.801211531221837
4	20.63824042772452	18.62244412924521	48.309902537522866	12.42941290550741
5	16.38343099861963	27.065516231704397	44.87266753621094	11.678385233465033
6	18.392382487936025	24.648157795667235	47.51894681203013	9.440512904366608
7	56.15405383822674	4.422507253596377	36.569153487240136	2.8542854209367503
8	56.3468492963156	4.616063246036665	36.40715967038442	2.6299277872633313
9	52.05249208093606	6.949002369064505	37.49396325858548	3.504542291413947
10	28.154601422959775	22.355907260441185	41.85943043582421	7.630060880774832
11	21.518178672335182	18.802690770535378	48.685606507131915	10.993524049997529
12	20.809360656797466	17.040152411084026	49.5917831868671	12.558703745251416
13	17.117346647754715	19.957181916014193	50.15914181303784	12.766329623193256
14	12.775836302586196	22.42967909253041	50.42494856886448	14.369536036018907
15	13.351180539447016	20.660676191091863	54.15689063135759	11.831252638103532
16	15.000399280534504	19.72864134340788	53.09632547828104	12.174633897776578
17	14.582105387245079	19.797849969388494	50.65881288193085	14.961231761435585
18	15.281036456214137	19.96744912975857	51.48513345476532	13.266380959261978
19	16.15907336494621	19.952998977081297	50.27626410315887	13.611663554813614
20	17.60827157160621	20.148076038224456	50.622307233061946	11.621345157107383
21	16.516904777296528	20.719997870503818	49.90094040072555	12.862156951474105
22	15.570039509759557	19.003852106490022	50.68505131705536	14.74105706669506
23	14.6988474101904	20.414643328402533	50.95123834005773	13.93527092134934
24	15.446072410475601	19.63243374795131	51.39881280587741	13.52268103569568
25	14.368395234491754	21.7102135960726	49.416099751685536	14.50529141775011
26	14.624695310925457	22.38290622991714	49.53246150745514	13.459936951702268
27	14.90229034919935	21.961570199221974	50.543211660512675	12.592927791066003
28	13.516596760884198	22.215208405425653	51.11627429431918	13.151920539370963
29	14.284356188658151	21.10254664927578	50.23633604970853	14.376761112357542
30	15.566617105178098	21.144376038604722	49.04952219429371	14.239484661923468
31	16.42716172382716	22.815650275883836	47.10369505614645	13.653492944142553
32	16.45301989177596	23.905876268666365	46.27167047567621	13.369433363881464
33	16.464808174223208	24.298311994006987	44.20301703977214	15.033862791997658
34	15.65750096017462	25.17216596380617	43.75582284112818	15.414510234891035
35	16.94394481562746	25.728877109056825	40.66006776361071	16.667110311705006
36	18.23913481612181	27.12788004852209	39.05914295383936	15.573842181516733
37	17.157274701205065	28.012761766417082	39.913983564852664	14.915979967525184
38	16.881580998809763	28.818547911762803	38.2480330680336	16.05183802139383
39	17.844417487726876	26.48294691850494	38.709297152179126	16.96333844158906
40	18.00184809847399	26.296996269579004	39.878618717510925	15.822536914436084
41	16.966000311819084	27.189103063812638	38.49824886965582	17.34664775471246
42	18.420141991763415	27.06969917063729	37.71679982355603	16.793359014043265
43	19.011837717180093	27.14917501036228	37.1197803576793	16.719206914778322
44	16.705517296452488	28.445505812383782	36.63798184604503	18.2109950451187
45	17.08084099888582	27.816163636571055	36.68399417430687	18.41900119023626
46	17.308621037140696	28.07740718628909	37.07528909812034	17.53868267844988
47	17.05308149505843	27.14118939967221	38.61308955672255	17.192639548546808
48	17.287706342476223	25.731158712111128	38.948865472881245	18.0322694725314
49	16.90667863240713	26.357458750518113	38.798659938472774	17.937202678601984
50	16.042711609176607	25.833830849554897	39.16942043479749	18.954037106471006
51	16.179607792434965	25.616698292220114	37.9076939457663	20.295999969578627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	361.0
1	735.0
2	1109.0
3	14845.5
4	28582.0
5	18470.5
6	8359.0
7	8004.5
8	7650.0
9	7402.5
10	7155.0
11	7068.5
12	6982.0
13	6537.0
14	6092.0
15	5608.5
16	5125.0
17	4742.0
18	4359.0
19	4106.5
20	3854.0
21	3648.5
22	3443.0
23	3373.5
24	3304.0
25	3332.0
26	3549.0
27	3738.0
28	4315.5
29	4893.0
30	5302.5
31	5712.0
32	6324.0
33	6936.0
34	7246.0
35	7556.0
36	7474.5
37	7393.0
38	8488.0
39	9583.0
40	10285.5
41	10988.0
42	11335.0
43	11682.0
44	12532.5
45	13383.0
46	14200.0
47	15017.0
48	14856.0
49	14695.0
50	13831.0
51	12967.0
52	11361.5
53	9756.0
54	8659.5
55	7563.0
56	6849.0
57	6135.0
58	6048.0
59	5961.0
60	5923.0
61	5885.0
62	5381.5
63	4878.0
64	4052.0
65	3226.0
66	2594.0
67	1962.0
68	1599.0
69	1236.0
70	1007.5
71	779.0
72	683.5
73	588.0
74	467.0
75	277.5
76	209.0
77	133.0
78	57.0
79	68.0
80	79.0
81	50.5
82	22.0
83	15.5
84	9.0
85	6.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.168151141285485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58373524876751	31.171477706117752
2	7.913201770862691	6.198909659452757
3	2.7039573690644096	3.1772703273332237
4	1.5552732159640572	2.4366870555549336
5	1.0144898167894956	1.9867845237652992
6	0.7995956429576062	1.8791209797166117
7	0.7116399649435443	1.9511535193561493
8	0.5727102427095822	1.7945601077288966
9	0.48875271954709987	1.7229186350941612
>10	4.469738571697548	34.103688698032656
>50	0.14892465276884304	3.6462767928612063
>100	0.035981795299854694	2.6331176410548074
>500	0.0	0.0
>1k	9.994943138848526E-4	0.42280211899185133
>5k	0.0	0.0
>10k+	9.994943138848526E-4	6.875232234939715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17562	6.678252139953532	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.4106885497750719	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	419	0.15933194662569922	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	387	0.14716339700273412	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	364	0.13841725196122795	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	297	0.11293935118814478	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	286	0.10875641225525053	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	270	0.10267213744376798	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07529290079209652	0.0
2	0.0	0.0	0.0	0.3684788932704118	0.0
3	0.0	0.0	0.0	0.5217265650846284	0.0
4	0.0	0.0	0.0	0.6890441224003985	0.0
5	0.0	0.0	0.0	1.2008837409163677	0.0
6	0.0	0.0	0.0	1.449578473835717	0.0
7	0.0	0.0	0.0	1.7188076342438197	0.0
8	0.0	0.0	0.0	2.1374817947089624	0.0
9	0.0	0.0	0.0	2.2971940085103792	0.0
10	0.0	0.0	0.0	2.6755598483494505	0.0
11	0.0	0.0	0.0	3.3680263753313078	0.0
12	0.0	0.0	0.0	3.863514505291418	0.0
13	3.802671757176592E-4	0.0	0.0	4.023606986268552	0.0
14	3.802671757176592E-4	0.0	0.0	4.082928665680507	0.0
15	3.802671757176592E-4	0.0	0.0	4.214501108478817	0.0
16	3.802671757176592E-4	0.0	0.0	4.457491833762401	0.0
17	3.802671757176592E-4	0.0	0.0	4.7951690857996825	0.0
18	3.802671757176592E-4	0.0	0.0	5.149958360744259	0.0
19	3.802671757176592E-4	0.0	0.0	5.369372521133348	0.0
20	3.802671757176592E-4	0.0	0.0	5.596772292212509	0.0
21	3.802671757176592E-4	0.0	0.0	5.88881748316367	0.0
22	3.802671757176592E-4	0.0	0.0	6.196833895494975	0.0
23	3.802671757176592E-4	0.0	0.0	6.519300460503549	0.0
24	3.802671757176592E-4	0.0	0.0	6.7455594300555575	0.0
25	3.802671757176592E-4	0.0	0.0	6.956607712578858	0.0
26	3.802671757176592E-4	0.0	0.0	7.144459697383382	0.0
27	3.802671757176592E-4	0.0	0.0	7.352085575325224	0.0
28	3.802671757176592E-4	0.0	0.0	7.560091720442783	0.0
29	3.802671757176592E-4	0.0	0.0	7.798138972442038	0.0
30	3.802671757176592E-4	0.0	0.0	8.073452407661623	0.0
31	3.802671757176592E-4	0.0	0.0	8.321006339053818	0.0
32	7.605343514353184E-4	0.0	0.0	8.55334958341731	0.0
33	7.605343514353184E-4	0.0	0.0	8.784552026253646	0.0
34	7.605343514353184E-4	0.0	0.0	9.02754275153723	0.0
35	7.605343514353184E-4	0.0	0.0	9.300954850878227	0.0
36	7.605343514353184E-4	0.0	0.0	9.550410118149012	0.0
37	7.605343514353184E-4	0.0	0.0	9.797964049541207	0.0
38	7.605343514353184E-4	0.0	0.0	10.058066797732087	0.0
39	0.001521068702870637	0.0	0.0	10.301437790191388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	30	2.159095E-6	45.000004	14
ACGGGAC	60	0.0	45.000004	5
CGGGAAC	30	2.159095E-6	45.000004	6
AACTGAT	30	2.159095E-6	45.000004	33
CGTAAGG	30	2.159095E-6	45.000004	2
CAATAAG	30	2.159095E-6	45.000004	1
GCGACAG	20	7.0233556E-4	45.0	1
ACCATAA	25	3.8825747E-5	45.0	28
CTATACG	25	3.8825747E-5	45.0	45
TGAACGG	20	7.0233556E-4	45.0	2
TTCGTAG	25	3.8825747E-5	45.0	1
TTGTAGT	20	7.0233556E-4	45.0	30
AGGGTCC	20	7.0233556E-4	45.0	6
CGACAAT	20	7.0233556E-4	45.0	20
ACGGGCG	25	3.8825747E-5	45.0	5
TCGGCAG	20	7.0233556E-4	45.0	1
TATGACG	25	3.8825747E-5	45.0	1
TGGTTGA	20	7.0233556E-4	45.0	14
GTTACGG	20	7.0233556E-4	45.0	2
CAGATAG	20	7.0233556E-4	45.0	45
>>END_MODULE