FastQCFastQC Report
Sat 18 Jun 2016
SRR3550863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588962
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174092.9558783079383733No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC11160.18948590910788812No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10660.18099639705108309No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10200.17318604595882248No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.17199751427086976No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG9010.1529810072636265No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8560.14534044641250202No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8180.13888841724933018No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7900.13413429049751938No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7230.12275834434140065No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC6430.10917512505051259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG453.8380676E-1045.0000041
TCTACGG207.0304825E-445.02
CCGACGA207.0304825E-445.018
AGTTACG253.8884813E-545.01
CGATAAG207.0304825E-445.010
GCCCGTT253.8884813E-545.044
TACCGCA253.8884813E-545.027
GCGTACG302.1636915E-644.9999961
CGTTATT5150.044.1262131
CGTTTTT85300.043.2590871
CGTAAGG750.042.0000042
CGTGGGA850.039.7058834
ACCACCG3700.039.5270314
TGGGCGA2300.039.1304366
CGATGAA2850.038.6842119
GTTAACG356.2431027E-638.5714261
AACGAGC356.2431027E-638.57142615
CGATATG356.2431027E-638.57142610
ATAAGGG2600.038.0769273
CTAGTAC301.13924616E-437.49999623
Produced by FastQC (version 0.11.5)