##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550863_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 588962 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19181203541145 33.0 31.0 34.0 30.0 34.0 2 32.263735181556704 34.0 31.0 34.0 30.0 34.0 3 32.12638336598965 33.0 31.0 34.0 30.0 34.0 4 35.885242171820934 37.0 35.0 37.0 35.0 37.0 5 33.87559299241717 37.0 35.0 37.0 32.0 37.0 6 34.82083394174836 37.0 35.0 37.0 32.0 37.0 7 35.834230391774 37.0 35.0 37.0 33.0 37.0 8 35.868575561751015 37.0 35.0 37.0 35.0 37.0 9 37.815707974368465 39.0 38.0 39.0 35.0 39.0 10 37.46043208220564 39.0 37.0 39.0 34.0 39.0 11 37.38407571286433 39.0 37.0 39.0 35.0 39.0 12 37.3917077842034 39.0 37.0 39.0 35.0 39.0 13 37.382442330744595 39.0 37.0 39.0 35.0 39.0 14 38.66391380089038 40.0 38.0 41.0 35.0 41.0 15 38.77812490449299 40.0 38.0 41.0 35.0 41.0 16 38.7258940305147 40.0 38.0 41.0 35.0 41.0 17 38.677622664959706 40.0 38.0 41.0 35.0 41.0 18 38.41723744486062 40.0 38.0 41.0 35.0 41.0 19 38.07753810941962 40.0 37.0 41.0 34.0 41.0 20 37.80399414563249 39.0 35.0 41.0 34.0 41.0 21 37.69641674675106 39.0 35.0 41.0 34.0 41.0 22 37.70360906136559 39.0 35.0 41.0 34.0 41.0 23 37.68178422376995 39.0 35.0 41.0 34.0 41.0 24 37.61110394218982 39.0 35.0 41.0 34.0 41.0 25 37.40589885255755 39.0 35.0 41.0 33.0 41.0 26 37.41440704154088 39.0 35.0 41.0 33.0 41.0 27 37.38713872881442 39.0 35.0 41.0 33.0 41.0 28 37.25341702860286 39.0 35.0 41.0 33.0 41.0 29 37.16528061233153 39.0 35.0 41.0 33.0 41.0 30 36.89791531541933 39.0 35.0 41.0 33.0 41.0 31 36.676269436737854 39.0 35.0 41.0 32.0 41.0 32 36.2382377810453 39.0 35.0 41.0 30.0 41.0 33 35.74522974317528 39.0 35.0 41.0 25.0 41.0 34 35.28507781486751 39.0 35.0 41.0 21.0 41.0 35 34.95798370692846 39.0 35.0 41.0 18.0 41.0 36 34.717898608059606 39.0 35.0 41.0 18.0 41.0 37 34.595439094542606 39.0 35.0 41.0 17.0 41.0 38 34.45621449261582 39.0 35.0 41.0 15.0 41.0 39 34.3345445037201 39.0 34.0 41.0 15.0 41.0 40 34.21721605129024 39.0 34.0 41.0 15.0 41.0 41 34.06480553923683 38.0 34.0 41.0 15.0 41.0 42 33.995639786607626 38.0 34.0 41.0 14.0 41.0 43 33.8834288120456 38.0 34.0 41.0 12.0 41.0 44 33.74112081933979 38.0 34.0 41.0 12.0 41.0 45 33.699953477473926 38.0 33.0 41.0 12.0 41.0 46 33.605730081057864 38.0 33.0 41.0 12.0 41.0 47 33.57802540741169 38.0 33.0 40.0 12.0 41.0 48 33.516287977832185 38.0 33.0 40.0 12.0 41.0 49 33.42656741861105 37.0 33.0 40.0 12.0 41.0 50 33.351177155741794 37.0 33.0 40.0 12.0 41.0 51 32.441485189197266 36.0 31.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 6.0 9 16.0 10 9.0 11 9.0 12 15.0 13 21.0 14 21.0 15 50.0 16 75.0 17 166.0 18 306.0 19 592.0 20 1018.0 21 1677.0 22 2509.0 23 3628.0 24 5703.0 25 9524.0 26 14669.0 27 17828.0 28 16853.0 29 14437.0 30 12959.0 31 12931.0 32 14128.0 33 18157.0 34 30903.0 35 41117.0 36 39104.0 37 54159.0 38 94377.0 39 181881.0 40 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.186650412080915 16.991249010971845 21.722454080229284 26.099646496717956 2 40.41822732196644 23.071946916779012 22.39754007898642 14.112285682268125 3 27.411785480217738 21.51310271290847 38.08463024779188 12.990481559081909 4 25.261222285987888 21.871020541223373 38.144905783395195 14.722851389393544 5 21.396626607489107 30.944271447054312 33.36938546120123 14.289716484255353 6 23.141051544921403 30.996906421806504 34.61751352379271 11.24452850947939 7 68.11492082681056 5.998010058373885 22.53371185237757 3.3533572624379846 8 69.97021879170472 6.166441977580896 21.143639148196318 2.7197000825180573 9 64.05897154655139 8.174720949738692 22.468851980263583 5.2974555234463345 10 35.10922606212286 24.91994390130433 28.9025437973927 11.068286239180116 11 26.932128049008252 22.095143659523025 36.555329545879026 14.417398745589699 12 24.921641803715687 20.33153242484235 38.351200926375554 16.395624845066408 13 21.210027132480533 21.901922365110142 39.657567041676714 17.23048346073261 14 17.365806282918083 24.222106010234956 39.320024042298144 19.092063664548817 15 17.72559180388548 23.379606833717624 41.956017535936105 16.938783826460792 16 19.936260743477508 22.100916527721655 40.855606983133036 17.1072157456678 17 19.348616718905465 23.67147625823058 39.31425117409952 17.665655848764438 18 19.22042508684771 23.517136929037864 39.236317453418046 18.026120530696378 19 21.903620267521504 22.612494524264722 37.66066401567503 17.82322119253874 20 22.64747131393876 22.910306607217443 39.196926117474476 15.245295961369324 21 21.56505852669612 23.725639345153 38.83815933795389 15.871142790196991 22 20.681639902064987 22.315701182758822 39.03596496887745 17.966693946298744 23 20.831564684988166 22.866161144522057 39.36094349041194 16.941330680077833 24 19.16728074137211 23.2205133777731 40.00903284082844 17.603173040026352 25 19.036372465456175 25.363605801392957 38.23744146481437 17.362580268336497 26 19.14113304423715 25.28550229047035 38.26596622532523 17.307398439967265 27 20.26514444055813 24.45845402589641 38.50689857749736 16.7695029560481 28 18.210342942329046 25.47210176547893 39.16398681069407 17.153568481497956 29 19.31567741212506 23.745335013124784 38.983329994125256 17.955657580624894 30 18.415449553621457 24.892268091999146 38.306546092956765 18.38573626142264 31 20.071074194939573 24.509900468960648 36.93430136409479 18.484723972004986 32 20.41812544782176 25.609801651040303 36.466868830247115 17.50520407089082 33 20.414390062516766 26.49220153422462 35.37392904805403 17.71947935520458 34 19.50261646761591 26.39830753087635 35.28088399591145 18.818192005596288 35 21.06519605679144 26.067895721625504 34.1857369405836 18.681171280999454 36 20.630533039483023 28.786916643179016 32.21871699702188 18.363833320316083 37 20.118275881975407 28.89694071943521 33.320995242477444 17.66378815611194 38 19.667652582000198 27.71961518739749 33.48552198613832 19.12721024446399 39 21.060441930039627 27.386656524529595 32.95153167776529 18.601369867665486 40 20.834620909328617 26.685762409119775 33.72051167987068 18.759105001680922 41 18.707658558616686 28.10809525911689 33.74156566977156 19.442680512494864 42 19.895171505122573 27.91979788169695 32.68750785279865 19.497522760381823 43 21.04516080833738 27.371884773550754 32.59310447872698 18.989849939384882 44 20.15478078381967 27.55797487783592 32.55625999640045 19.730984341943962 45 19.965974035676325 27.467646469551514 32.39937381359069 20.167005681181468 46 19.267456983642408 28.602694231546348 32.70873163294066 19.42111715187058 47 20.309120113012387 26.974066238568874 33.92154332537583 18.795270323042914 48 19.534537032949494 26.595773581317637 34.47234286762134 19.397346518111526 49 18.948930491271085 27.435556113976794 34.2877808755064 19.327732519245725 50 18.592201194644137 27.364923373664173 34.21850645712287 19.824368974568817 51 19.262193486167188 25.99284843504335 33.70557013865071 21.039387940138752 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 385.0 1 1292.0 2 2199.0 3 17398.5 4 32598.0 5 21996.0 6 11394.0 7 10657.5 8 9921.0 9 9348.0 10 8775.0 11 8350.5 12 7926.0 13 7585.5 14 7245.0 15 6741.0 16 6237.0 17 5819.0 18 5401.0 19 5175.0 20 4949.0 21 4830.0 22 4711.0 23 4996.5 24 5282.0 25 5741.5 26 6963.0 27 7725.0 28 9727.5 29 11730.0 30 13169.5 31 14609.0 32 15735.0 33 16861.0 34 17823.5 35 18786.0 36 19854.0 37 20922.0 38 22539.5 39 24157.0 40 25981.5 41 27806.0 42 28711.0 43 29616.0 44 31778.5 45 33941.0 46 34993.0 47 36045.0 48 34863.5 49 33682.0 50 31610.5 51 29539.0 52 27993.0 53 26447.0 54 25098.0 55 23749.0 56 22982.5 57 22216.0 58 21639.5 59 21063.0 60 20670.5 61 20278.0 62 19206.0 63 18134.0 64 15487.0 65 12840.0 66 10572.0 67 8304.0 68 7102.0 69 5900.0 70 5067.0 71 4234.0 72 3574.0 73 2914.0 74 2470.0 75 1575.0 76 1124.0 77 862.0 78 600.0 79 428.0 80 256.0 81 198.0 82 140.0 83 95.0 84 50.0 85 32.5 86 15.0 87 19.0 88 23.0 89 12.5 90 2.0 91 2.0 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 588962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.627922473808354 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38664096952986 22.78308272701777 2 7.149256765030257 4.379337638893933 3 3.0357467065684594 2.7893584433669347 4 1.9353400727597996 2.3710178283576706 5 1.4083551658250795 2.1567496417239025 6 1.1839780520942251 2.1757672794139533 7 1.0477565257012735 2.246342394842378 8 0.906268417538399 2.2205695066261653 9 0.8157786369700746 2.2487044364017645 >10 7.910494198719169 46.44147708820543 >50 0.17630759141064828 3.3370232718830697 >100 0.0379396082782402 2.3469212216326043 >500 0.003347612495138841 0.8084523299507682 >1k 0.0022317416634258943 0.7202761722135886 >5k 0.0 0.0 >10k+ 5.579354158564736E-4 2.974920019470075 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17409 2.9558783079383733 No Hit GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC 1116 0.18948590910788812 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1066 0.18099639705108309 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1020 0.17318604595882248 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1013 0.17199751427086976 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG 901 0.1529810072636265 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 856 0.14534044641250202 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 818 0.13888841724933018 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 790 0.13413429049751938 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 723 0.12275834434140065 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC 643 0.10917512505051259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.093707234083013E-4 0.0 0.0 0.020544619177468155 0.0 2 5.093707234083013E-4 0.0 0.0 0.13515303194433598 0.0 3 5.093707234083013E-4 0.0 0.0 0.17369541668223076 0.0 4 5.093707234083013E-4 0.0 0.0 0.27217375654116904 0.0 5 6.791609645444019E-4 0.0 0.0 0.6041136779622455 0.0 6 6.791609645444019E-4 0.0 0.0 0.7270418125447822 0.0 7 6.791609645444019E-4 0.0 0.0 0.8717030979927398 0.0 8 6.791609645444019E-4 0.0 0.0 1.0489641097388287 0.0 9 6.791609645444019E-4 0.0 0.0 1.1258790889734822 0.0 10 6.791609645444019E-4 0.0 0.0 1.4338785863943684 0.0 11 6.791609645444019E-4 0.0 0.0 1.6515496755308492 0.0 12 6.791609645444019E-4 0.0 0.0 1.9279681881004207 0.0 13 8.489512056805023E-4 0.0 0.0 2.006241489264163 0.0 14 8.489512056805023E-4 0.0 0.0 2.0412182789382 0.0 15 8.489512056805023E-4 0.0 0.0 2.1177936776905812 0.0 16 8.489512056805023E-4 0.0 0.0 2.219158451648833 0.0 17 8.489512056805023E-4 0.0 0.0 2.330880430316387 0.0 18 0.0010187414468166027 0.0 0.0 2.455506467310285 0.0 19 0.0010187414468166027 0.0 0.0 2.574189845864419 0.0 20 0.0010187414468166027 0.0 0.0 2.677592102716304 0.0 21 0.0010187414468166027 0.0 0.0 2.8011993982633854 0.0 22 0.0011885316879527033 0.0 0.0 2.9416159276829403 0.0 23 0.0011885316879527033 0.0 0.0 3.0686190280527437 0.0 24 0.0011885316879527033 0.0 0.0 3.1696442215287233 0.0 25 0.0011885316879527033 0.0 0.0 3.265745498011756 0.0 26 0.0011885316879527033 0.0 0.0 3.356753067260706 0.0 27 0.0011885316879527033 0.0 0.0 3.45064707060897 0.0 28 0.0011885316879527033 0.0 0.0 3.545729605645186 0.0 29 0.0011885316879527033 0.0 0.0 3.6467547991211657 0.0 30 0.0011885316879527033 0.0 0.0 3.7611934216468974 0.0 31 0.0011885316879527033 0.0 0.0 3.88038617092444 0.0 32 0.0011885316879527033 0.0 0.0 4.001955983577888 0.0 33 0.0011885316879527033 0.0 0.0 4.108584255011359 0.0 34 0.0011885316879527033 0.0 0.0 4.22217392633141 0.0 35 0.0011885316879527033 0.0 0.0 4.34408331946713 0.0 36 0.0013583219290888037 0.0 0.0 4.474652014900792 0.0 37 0.0013583219290888037 0.0 0.0 4.610314417568536 0.0 38 0.0015281121702249041 0.0 0.0 4.735789405768114 0.0 39 0.0015281121702249041 0.0 0.0 4.86873516457768 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAG 45 3.8380676E-10 45.000004 1 TCTACGG 20 7.0304825E-4 45.0 2 CCGACGA 20 7.0304825E-4 45.0 18 AGTTACG 25 3.8884813E-5 45.0 1 CGATAAG 20 7.0304825E-4 45.0 10 GCCCGTT 25 3.8884813E-5 45.0 44 TACCGCA 25 3.8884813E-5 45.0 27 GCGTACG 30 2.1636915E-6 44.999996 1 CGTTATT 515 0.0 44.126213 1 CGTTTTT 8530 0.0 43.259087 1 CGTAAGG 75 0.0 42.000004 2 CGTGGGA 85 0.0 39.705883 4 ACCACCG 370 0.0 39.52703 14 TGGGCGA 230 0.0 39.130436 6 CGATGAA 285 0.0 38.68421 19 GTTAACG 35 6.2431027E-6 38.571426 1 AACGAGC 35 6.2431027E-6 38.571426 15 CGATATG 35 6.2431027E-6 38.571426 10 ATAAGGG 260 0.0 38.076927 3 CTAGTAC 30 1.13924616E-4 37.499996 23 >>END_MODULE