FastQCFastQC Report
Sat 18 Jun 2016
SRR3550860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418986
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC78711.8785830552810836No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC67071.600769476784427No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG64481.5389535688543292No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC31160.7437002668346914No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT14660.3498923591719055No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT14470.3453576014473037No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC13070.3119435971607643No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT11830.282348336221258No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA11290.26946007742502137No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC11130.2656413340779883No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.22220312850548704No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT7850.18735709546381024No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT5310.12673454482966018No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC4340.10358341328827216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC750.045.00000426
TTAGGCG253.886547E-545.01
TCGTTGA207.028148E-445.024
CGACGTT207.028148E-445.027
CACGGAC207.028148E-445.018
CGACGGT207.028148E-445.028
GCGAACC351.2094642E-745.033
ATGTGCG207.028148E-445.01
TAACGCA207.028148E-445.09
GCGCAGA207.028148E-445.010
GTTAACG207.028148E-445.01
CGAATGG351.2094642E-745.02
CATCGAA207.028148E-445.042
CCCGGTC207.028148E-445.04
CGCGAGG1050.042.8571432
GACACGA800.042.187525
AACACGT800.042.187541
TACGGCT8900.041.966297
TCGATGG603.6379788E-1241.2500042
AGTACGG603.6379788E-1241.2500042