##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550860_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	418986
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354329261598238	31.0	31.0	33.0	28.0	34.0
2	31.261657907424116	31.0	31.0	34.0	28.0	34.0
3	31.213852014148443	31.0	31.0	34.0	28.0	34.0
4	35.07675435456077	35.0	35.0	37.0	32.0	37.0
5	35.31423484316898	35.0	35.0	37.0	33.0	37.0
6	35.2457003336627	37.0	35.0	37.0	33.0	37.0
7	35.592950599781375	37.0	35.0	37.0	33.0	37.0
8	35.22312440033796	37.0	35.0	37.0	32.0	37.0
9	37.1427135990224	39.0	37.0	39.0	34.0	39.0
10	36.580649472774745	38.0	35.0	39.0	32.0	39.0
11	36.716735165375454	39.0	35.0	39.0	32.0	39.0
12	36.88476941950328	39.0	37.0	39.0	33.0	39.0
13	36.88951420811197	39.0	37.0	39.0	33.0	39.0
14	37.923641840061485	40.0	37.0	41.0	33.0	41.0
15	37.97766035141986	40.0	37.0	41.0	33.0	41.0
16	38.10622311962691	40.0	37.0	41.0	34.0	41.0
17	37.90563407846563	40.0	37.0	41.0	33.0	41.0
18	37.89779372103125	39.0	37.0	41.0	33.0	41.0
19	37.80551617476479	39.0	37.0	41.0	33.0	41.0
20	37.772321748220705	40.0	36.0	41.0	33.0	41.0
21	37.80090265545866	39.0	36.0	41.0	33.0	41.0
22	37.9367425164564	40.0	37.0	41.0	33.0	41.0
23	37.97608273307461	40.0	37.0	41.0	34.0	41.0
24	37.90377005436936	40.0	36.0	41.0	33.0	41.0
25	37.6740487748994	39.0	36.0	41.0	33.0	41.0
26	37.71792374924222	40.0	36.0	41.0	33.0	41.0
27	37.77062956757505	40.0	37.0	41.0	33.0	41.0
28	37.660568610884376	40.0	36.0	41.0	33.0	41.0
29	37.71416706047457	40.0	36.0	41.0	33.0	41.0
30	37.475908502909405	40.0	36.0	41.0	33.0	41.0
31	37.46131135646537	40.0	36.0	41.0	33.0	41.0
32	37.40482020878979	40.0	36.0	41.0	33.0	41.0
33	37.38307246542844	40.0	36.0	41.0	32.0	41.0
34	37.35656322645625	40.0	36.0	41.0	33.0	41.0
35	37.31402242557031	40.0	36.0	41.0	33.0	41.0
36	37.24624211787506	40.0	36.0	41.0	32.0	41.0
37	37.2771238179796	40.0	36.0	41.0	33.0	41.0
38	37.07408123421785	40.0	36.0	41.0	32.0	41.0
39	37.08784064384013	40.0	36.0	41.0	32.0	41.0
40	36.95126328803349	40.0	35.0	41.0	31.0	41.0
41	36.781627548414505	39.0	35.0	41.0	31.0	41.0
42	36.89673401975245	39.0	35.0	41.0	31.0	41.0
43	36.87000520303781	39.0	35.0	41.0	31.0	41.0
44	36.83409469528815	39.0	35.0	41.0	31.0	41.0
45	36.79822476168655	39.0	35.0	41.0	31.0	41.0
46	36.687416763328606	39.0	35.0	41.0	31.0	41.0
47	36.58827263918126	39.0	35.0	41.0	31.0	41.0
48	36.55106375869361	39.0	35.0	41.0	31.0	41.0
49	36.56058197648609	39.0	35.0	41.0	31.0	41.0
50	36.413901657811955	39.0	35.0	41.0	31.0	41.0
51	35.21722682858138	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	11.0
10	16.0
11	18.0
12	6.0
13	5.0
14	12.0
15	16.0
16	33.0
17	64.0
18	125.0
19	298.0
20	546.0
21	1054.0
22	1511.0
23	1895.0
24	2157.0
25	2632.0
26	3169.0
27	3518.0
28	3918.0
29	4694.0
30	6040.0
31	8017.0
32	10837.0
33	15087.0
34	25556.0
35	29105.0
36	32711.0
37	50554.0
38	94360.0
39	120993.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.44642064412653	19.538839006553918	21.427446263120963	12.587294086198582
2	28.97710186020535	30.709617982462422	23.412238117741406	16.901042039590823
3	30.172845870745086	29.780708663296622	24.61681297227115	15.42963249368714
4	27.761070775634504	23.56976128080652	31.417756201877868	17.251411741681107
5	29.55516413436248	28.023609380743032	22.917233511382243	19.50399297351224
6	24.487930384308783	39.00201916054474	22.5270056756073	13.983044779539172
7	75.04952432778184	5.442902626818079	15.053247602545191	4.454325442854892
8	74.86073520356288	10.604172931792471	9.114624354990381	5.4204675096542605
9	69.56079678079936	7.4081234217849765	10.189600607180193	12.841479190235471
10	40.05503763848912	29.424372174726603	17.388170487796728	13.132419698987555
11	28.962542901194787	24.002949979235584	27.722167327786607	19.31233979178302
12	25.998959392437932	20.462497553617542	30.9754979879996	22.563045065944927
13	25.136162067467648	22.407908617471705	33.712582281985554	18.743347033075093
14	19.213052464760157	29.58308869508766	27.84675382948356	23.357105010668615
15	16.911543583795165	26.70518824017986	35.8162325232824	20.567035652742575
16	19.780613194712952	26.31352837564978	29.128896908249917	24.77696152138735
17	19.149804528074924	27.61357181385535	30.422496217057372	22.81412744101235
18	19.54170306406419	26.875599662041218	29.613877313323115	23.968819960571473
19	21.02265946833546	27.803554295370255	27.353181251879537	23.820604984414754
20	24.694858539426136	26.7958833946719	30.945186712682528	17.56407135321944
21	23.340398008525344	29.642756559885058	28.08566396013232	18.931181471457283
22	20.689235439847632	24.091497090594913	29.906488522289525	25.312778947267926
23	20.80522976901376	29.05896617070737	29.020778737237045	21.11502532304182
24	22.264467070498775	25.503477443160392	27.813339825197026	24.418715661143807
25	19.8367009876225	32.01204813525989	25.64763500451089	22.503615872606723
26	20.34817392466574	27.504737628464916	28.76993503362881	23.377153413240535
27	23.290277002095536	26.912116395297218	27.234561536662323	22.563045065944927
28	18.272209572634885	26.842901672132243	31.995341133116618	22.88954762211625
29	23.48455556987584	24.399144601490264	28.943926527378004	23.172373301255888
30	21.327442921720536	27.84866320115708	28.002128949415972	22.821764927706415
31	21.823640885375646	26.70709761185338	27.1092590205878	24.360002482183177
32	23.013179437976447	28.723871442005223	27.16844954246681	21.094499577551517
33	20.22191672275446	28.83342164177323	25.54500627705938	25.399655358412932
34	22.358742296878656	27.23145880769286	28.19235010239006	22.21744879303843
35	20.451279995035634	25.354785124085293	25.846448330015797	28.347486550863277
36	21.461098938866694	29.668533077477527	26.930255426195625	21.940112557460154
37	19.22498603771964	26.43620550567322	30.995785062030713	23.343023394576427
38	18.884401865456123	27.982557889762425	28.365864253220874	24.767175991560578
39	21.40763653200823	27.29041065811268	29.073525129717936	22.22842768016115
40	20.908335839383653	27.27060092699995	28.463480880029408	23.35758235358699
41	18.173399588530405	31.120610235186856	26.284649129087846	24.421341047194893
42	20.17251173070222	25.262419269378928	29.58643009551632	24.978638904402533
43	23.095521091396847	27.048397798494463	26.272954227587558	23.583126882521135
44	20.792341510217526	25.143799554161717	29.36518165284759	24.698677282773172
45	20.186832018253593	23.545178120509995	28.05821674232552	28.209773118910896
46	24.773142778040317	26.56890683698262	25.89060254996587	22.767347835011194
47	18.485104514231978	25.642861575327096	33.172707441298755	22.699326469142168
48	20.3262161504203	25.966977416906534	27.564166821803116	26.142639610870056
49	19.67010830910818	23.53682461943836	32.94811759820137	23.84494947325209
50	20.851532032096536	23.709384084432415	29.98835283279155	25.450731050679497
51	19.711398471547977	23.506036001202904	26.881566448520957	29.90099907872817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	329.5
2	547.0
3	1205.5
4	1864.0
5	1328.5
6	793.0
7	788.5
8	784.0
9	775.5
10	767.0
11	758.0
12	749.0
13	723.5
14	698.0
15	713.5
16	729.0
17	775.5
18	822.0
19	957.0
20	1092.0
21	1230.5
22	1369.0
23	1397.5
24	1426.0
25	1854.5
26	2965.0
27	3647.0
28	4432.5
29	5218.0
30	6292.0
31	7366.0
32	8120.5
33	8875.0
34	9600.5
35	10326.0
36	12270.5
37	14215.0
38	15264.5
39	16314.0
40	17972.5
41	19631.0
42	22046.0
43	24461.0
44	41623.0
45	58785.0
46	50546.0
47	42307.0
48	42161.0
49	42015.0
50	36346.5
51	30678.0
52	27317.5
53	23957.0
54	22071.0
55	20185.0
56	18818.5
57	17452.0
58	16196.0
59	14940.0
60	14340.0
61	13740.0
62	12596.5
63	11453.0
64	9513.0
65	7573.0
66	6030.0
67	4487.0
68	3520.0
69	2553.0
70	2176.5
71	1800.0
72	1507.0
73	1214.0
74	949.5
75	623.5
76	562.0
77	419.0
78	276.0
79	183.5
80	91.0
81	98.0
82	105.0
83	60.5
84	16.0
85	12.0
86	8.0
87	6.5
88	5.0
89	5.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	418986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.668629355329998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85612634023957	23.07253661334142
2	10.254789923622385	6.495102824159401
3	3.4883160509888764	3.3141056427904547
4	1.788432058439602	2.2654876794365455
5	1.1574981341088797	1.8328189694290085
6	0.9039307321284183	1.7175748391200178
7	0.7688373858745281	1.7043618345446883
8	0.6463369913565735	1.6374885294328365
9	0.5828678287703735	1.6612762709227415
>10	7.445656885051218	45.081483580731415
>50	0.07172344080885835	1.5902072370992864
>100	0.025669441973696673	1.4902663823244402
>500	0.0022649507623850006	0.5372418676532997
>1k	0.005284885112231667	2.572879278067271
>5k	0.0022649507623850006	5.027168450947165
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	7871	1.8785830552810836	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	6707	1.600769476784427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	6448	1.5389535688543292	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	3116	0.7437002668346914	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	1466	0.3498923591719055	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	1447	0.3453576014473037	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1307	0.3119435971607643	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1183	0.282348336221258	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	1129	0.26946007742502137	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	1113	0.2656413340779883	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.22220312850548704	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	785	0.18735709546381024	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	531	0.12673454482966018	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	434	0.10358341328827216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3867145918956718E-4	0.0	0.0	0.193562553402739	0.0
2	2.3867145918956718E-4	0.0	0.0	1.4950380203634488	0.0
3	2.3867145918956718E-4	0.0	0.0	1.8339514924126343	0.0
4	2.3867145918956718E-4	0.0	0.0	2.53111082470536	0.0
5	2.3867145918956718E-4	0.0	0.0	5.358174258805784	0.0
6	2.3867145918956718E-4	0.0	0.0	6.168225191295175	0.0
7	2.3867145918956718E-4	0.0	0.0	7.162530490278911	0.0
8	2.3867145918956718E-4	0.0	0.0	8.4370360823512	0.0
9	2.3867145918956718E-4	0.0	0.0	8.802919429288806	0.0
10	4.7734291837913436E-4	0.0	0.0	11.192020735776374	0.0
11	4.7734291837913436E-4	0.0	0.0	12.477027872053004	0.0
12	4.7734291837913436E-4	0.0	0.0	15.449919567718252	0.0
13	4.7734291837913436E-4	0.0	0.0	15.944208159699846	0.0
14	4.7734291837913436E-4	0.0	0.0	16.17309408906264	0.0
15	4.7734291837913436E-4	0.0	0.0	16.76476063639358	0.0
16	4.7734291837913436E-4	0.0	0.0	17.45332779615548	0.0
17	4.7734291837913436E-4	0.0	0.0	18.30323686232953	0.0
18	4.7734291837913436E-4	0.0	0.0	19.150043199534114	0.0
19	4.7734291837913436E-4	0.0	0.0	20.053892015485005	0.0
20	4.7734291837913436E-4	0.0	0.0	20.631238275264568	0.0
21	4.7734291837913436E-4	0.0	0.0	21.265865685249626	0.0
22	4.7734291837913436E-4	0.0	0.0	22.049901428687356	0.0
23	4.7734291837913436E-4	0.0	0.0	22.711498713560836	0.0
24	4.7734291837913436E-4	0.0	0.0	23.225358365195973	0.0
25	4.7734291837913436E-4	0.0	0.0	23.691961067911578	0.0
26	4.7734291837913436E-4	0.0	0.0	24.1146482221363	0.0
27	4.7734291837913436E-4	0.0	0.0	24.608220799740327	0.0
28	4.7734291837913436E-4	0.0	0.0	25.03162396834262	0.0
29	4.7734291837913436E-4	0.0	0.0	25.519945773844473	0.0
30	4.7734291837913436E-4	0.0	0.0	26.036192140071506	0.0
31	4.7734291837913436E-4	0.0	0.0	26.499692113817645	0.0
32	4.7734291837913436E-4	0.0	0.0	26.97250027447218	0.0
33	4.7734291837913436E-4	0.0	0.0	27.420247931911806	0.0
34	4.7734291837913436E-4	0.0	0.0	27.877542447719016	0.0
35	4.7734291837913436E-4	0.0	0.0	28.346293193567327	0.0
36	4.7734291837913436E-4	0.0	0.0	28.814089253578878	0.0
37	4.7734291837913436E-4	0.0	0.0	29.272577126682037	0.0
38	4.7734291837913436E-4	0.0	0.0	29.74108920107116	0.0
39	4.7734291837913436E-4	0.0	0.0	30.20840791816433	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	75	0.0	45.000004	26
TTAGGCG	25	3.886547E-5	45.0	1
TCGTTGA	20	7.028148E-4	45.0	24
CGACGTT	20	7.028148E-4	45.0	27
CACGGAC	20	7.028148E-4	45.0	18
CGACGGT	20	7.028148E-4	45.0	28
GCGAACC	35	1.2094642E-7	45.0	33
ATGTGCG	20	7.028148E-4	45.0	1
TAACGCA	20	7.028148E-4	45.0	9
GCGCAGA	20	7.028148E-4	45.0	10
GTTAACG	20	7.028148E-4	45.0	1
CGAATGG	35	1.2094642E-7	45.0	2
CATCGAA	20	7.028148E-4	45.0	42
CCCGGTC	20	7.028148E-4	45.0	4
CGCGAGG	105	0.0	42.857143	2
GACACGA	80	0.0	42.1875	25
AACACGT	80	0.0	42.1875	41
TACGGCT	890	0.0	41.96629	7
TCGATGG	60	3.6379788E-12	41.250004	2
AGTACGG	60	3.6379788E-12	41.250004	2
>>END_MODULE