FastQCFastQC Report
Sat 18 Jun 2016
SRR3550859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494006
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC75651.5313579187297321No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC51901.0505945271919774No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG44660.9040376027821524No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.7388574227843386No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC26430.5350137447723307No Hit
GAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT21710.43946834653830114No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC13340.27003720602583775No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG11900.2408877624968118No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT11260.22793245426168912No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT10990.22246693359999678No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT10580.21416743926187132No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC9630.19493690360036114No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCCAGGTT9580.19392477014449216No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT9530.1929126366886232Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT8890.17995732845350057No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC8660.17530151455650336No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT6650.1346137496305713No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTA6630.1342088962482237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT207.0293754E-445.00000422
CCGATAC207.0293754E-445.00000410
GGCGTAA207.0293754E-445.0000048
AAACGGT207.0293754E-445.00000438
TCGGAGA207.0293754E-445.00000429
GTGTACG207.0293754E-445.0000041
ACCACGA207.0293754E-445.00000419
CGATCCC207.0293754E-445.00000434
ACGAACG207.0293754E-445.0000041
ATTACGG253.8875653E-545.02
TTACGCG253.8875653E-545.01
TACACGG253.8875653E-545.02
CGCTCGA302.1629803E-644.99999641
CGTTTTT25950.043.6127171
TCAGACG650.041.53846422
GATAGCG556.002665E-1140.9090921
CCGATGA3450.040.43478418
TACGGCT5900.040.0423747
CCGGGAT451.9244908E-840.05
ACGGCTG6100.039.0983628
Produced by FastQC (version 0.11.5)