##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494006
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2896908134719	31.0	31.0	33.0	28.0	34.0
2	31.22214507516103	31.0	31.0	34.0	28.0	34.0
3	31.104824637757435	31.0	31.0	34.0	28.0	34.0
4	34.95287911482856	35.0	35.0	37.0	32.0	37.0
5	35.253345101071645	35.0	35.0	37.0	33.0	37.0
6	35.20201778925762	36.0	35.0	37.0	33.0	37.0
7	35.518524066509315	37.0	35.0	37.0	33.0	37.0
8	35.29373125022773	37.0	35.0	37.0	32.0	37.0
9	37.20611692975389	39.0	37.0	39.0	34.0	39.0
10	36.426557572175234	38.0	35.0	39.0	32.0	39.0
11	36.68243503115347	39.0	35.0	39.0	32.0	39.0
12	36.89173613275952	39.0	37.0	39.0	33.0	39.0
13	36.9963097614199	39.0	37.0	39.0	33.0	39.0
14	38.03417569827087	40.0	37.0	41.0	33.0	41.0
15	38.09757776221341	40.0	37.0	41.0	33.0	41.0
16	38.14057926421946	40.0	37.0	41.0	33.0	41.0
17	37.997789500532384	40.0	37.0	41.0	33.0	41.0
18	37.90354368165569	39.0	37.0	41.0	33.0	41.0
19	37.70590438172816	39.0	37.0	41.0	33.0	41.0
20	37.67499382598592	39.0	36.0	41.0	33.0	41.0
21	37.64257923992826	39.0	36.0	41.0	33.0	41.0
22	37.820749140698695	39.0	36.0	41.0	33.0	41.0
23	37.88161682246774	40.0	36.0	41.0	34.0	41.0
24	37.78062817050805	40.0	36.0	41.0	33.0	41.0
25	37.50836629514621	39.0	36.0	41.0	33.0	41.0
26	37.57897272502763	39.0	36.0	41.0	33.0	41.0
27	37.66961130026761	39.0	36.0	41.0	33.0	41.0
28	37.57402339242843	40.0	36.0	41.0	33.0	41.0
29	37.601589859232476	40.0	36.0	41.0	33.0	41.0
30	37.306271583745946	39.0	35.0	41.0	32.0	41.0
31	37.243685299368835	39.0	36.0	41.0	32.0	41.0
32	37.0743978818071	39.0	35.0	41.0	32.0	41.0
33	36.87767962332441	40.0	35.0	41.0	31.0	41.0
34	36.72866726315065	40.0	35.0	41.0	31.0	41.0
35	36.59213855702158	40.0	35.0	41.0	30.0	41.0
36	36.330378578397834	39.0	35.0	41.0	30.0	41.0
37	36.400410116476316	39.0	35.0	41.0	30.0	41.0
38	36.25485925272163	39.0	35.0	41.0	30.0	41.0
39	36.2200013765015	39.0	35.0	41.0	30.0	41.0
40	36.08676412837091	39.0	35.0	41.0	29.0	41.0
41	35.897940510843995	39.0	35.0	41.0	27.0	41.0
42	35.95504508042413	39.0	35.0	41.0	28.0	41.0
43	35.89535754626462	39.0	35.0	41.0	28.0	41.0
44	35.8997684238653	39.0	35.0	41.0	28.0	41.0
45	35.93882867819419	39.0	35.0	41.0	28.0	41.0
46	35.812771504799535	39.0	35.0	40.0	28.0	41.0
47	35.71763500848168	39.0	35.0	40.0	27.0	41.0
48	35.63137087403797	39.0	35.0	40.0	27.0	41.0
49	35.65511350064574	39.0	35.0	40.0	27.0	41.0
50	35.51942486528504	39.0	35.0	40.0	26.0	41.0
51	34.373714084444316	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	11.0
11	14.0
12	12.0
13	12.0
14	13.0
15	26.0
16	46.0
17	82.0
18	142.0
19	331.0
20	666.0
21	1152.0
22	1727.0
23	2387.0
24	3452.0
25	5235.0
26	6706.0
27	7236.0
28	6841.0
29	6978.0
30	8202.0
31	10172.0
32	13006.0
33	17922.0
34	29443.0
35	33696.0
36	39176.0
37	61229.0
38	111642.0
39	126416.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.515665801629936	18.838232734015374	21.24792006574819	16.398181398606496
2	34.246750039473206	26.96748622486367	22.626243405950536	16.159520329712596
3	28.967461933660726	26.608988554794884	28.986692469322232	15.43685704222216
4	25.92559604539216	22.83676716477128	33.63744569904009	17.600191090796468
5	25.762440132306086	27.343797443755747	28.449249604255815	18.444512819682352
6	22.995267263960358	35.33337651769411	27.729217863750645	13.942138354594883
7	73.65193945012814	5.08657789581503	16.526924774193024	4.734557879863807
8	74.23249920041457	7.176431055493253	13.11704716137051	5.474022582721667
9	69.58457994437315	5.53130933632385	14.37128293988332	10.512827779419682
10	37.01675688149537	21.11391359619114	24.904353388420382	16.96497613389311
11	27.857758812646	24.36427897636871	27.262826767286224	20.515135443699066
12	22.39264300433598	20.977073152957658	33.603033161540544	23.027250681165814
13	22.096695181839895	22.311672327866464	37.199143330242954	18.392489160050687
14	20.330319874657395	27.045218074274402	32.01499576928216	20.60946628178605
15	18.420626470123842	25.724991194438935	36.75866285024878	19.09571948518844
16	20.480925332890692	25.726813034659497	31.17006676032275	22.62219487212706
17	20.03376477208779	26.65109330655903	31.571478888920378	21.743663032432806
18	20.29023938980498	26.054744274361042	31.724108614065415	21.930907721768563
19	20.18214353671818	28.475362647417242	29.379805103581734	21.962688712282848
20	21.079905912073944	27.469504418974665	32.25102529119079	19.199564377760595
21	21.28435687015947	28.250061740140808	31.846779188916734	18.618802200782987
22	19.42203131136059	24.45192973364696	31.582612356934938	24.543426598057515
23	19.705833532386247	27.074772371185773	31.376339558628842	21.843054537799137
24	20.899543730238094	25.67985004230718	30.361372129083453	23.059234098371277
25	19.45948024922774	30.17493714651239	28.46422917940268	21.901353424857188
26	19.217377926583886	25.89887572215722	31.089905790617927	23.793840560640962
27	21.729290737359467	25.861021930907725	30.177771120188822	22.23191621154399
28	18.05200746549637	26.051505447302258	31.42856564495168	24.46792144224969
29	19.93761209378024	23.36975664263187	29.803079314826135	26.88955194876176
30	19.666157900916183	25.997255094067683	31.439294259583896	22.89729274543224
31	20.17991684311527	24.831277352906646	27.651890867722255	27.336914936255834
32	20.926668906855383	26.122759642595433	27.336307656182314	25.61426379436687
33	19.65158317915167	26.58064881803055	28.13791735323053	25.629850649587254
34	22.571993052715957	24.878442771950137	27.20209875993409	25.34746541539981
35	22.05580499022279	24.125010627401284	28.568276498666005	25.250907883709917
36	20.800961931636458	25.985514345979606	28.971712894175372	24.241810828208564
37	20.743675178034273	24.643425383497366	31.482208718112737	23.130690720355624
38	20.38153382752436	26.981251239863486	27.096432027141372	25.54078290547078
39	22.991421156828054	24.471969976073165	28.33953433763962	24.197074529459154
40	23.092432075723774	25.181070675254958	29.419683161742977	22.306814087278294
41	21.71289417537439	25.28208159415068	29.61057153151986	23.394452698955075
42	22.26288749529358	23.906187374242418	31.15205078480828	22.67887434565572
43	21.28476172354182	23.483115589689195	30.546794978198644	24.68532770857034
44	20.920393679428994	24.128047027768893	29.01746132638065	25.93409796642146
45	20.965129978178403	23.106197090723594	27.61970502382562	28.308967907272383
46	24.069748140710843	24.623182714379986	27.52658874588568	23.780480399023492
47	20.151577106350935	23.788982320052792	32.61883458905358	23.4406059845427
48	20.688210264652657	23.943231458727222	29.540329469682554	25.82822880693757
49	20.803998332004063	22.09790974198694	32.9500046558139	24.1480872701951
50	20.70238013303482	21.710465055080302	31.409335109290172	26.177819702594707
51	20.1742488957624	21.584758079861377	28.85066173285345	29.390331291522777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	363.5
2	533.0
3	3451.0
4	6369.0
5	4505.0
6	2641.0
7	2597.0
8	2553.0
9	2526.0
10	2499.0
11	2517.0
12	2535.0
13	2473.5
14	2412.0
15	2319.0
16	2226.0
17	2132.5
18	2039.0
19	1959.0
20	1879.0
21	1838.5
22	1798.0
23	1856.5
24	1915.0
25	2422.5
26	3297.0
27	3664.0
28	4347.5
29	5031.0
30	5781.0
31	6531.0
32	7653.5
33	8776.0
34	9913.0
35	11050.0
36	12186.0
37	13322.0
38	15083.5
39	16845.0
40	18580.5
41	20316.0
42	21939.5
43	23563.0
44	25694.0
45	27825.0
46	30405.0
47	32985.0
48	49931.5
49	66878.0
50	60427.5
51	53977.0
52	52314.0
53	50651.0
54	42705.5
55	34760.0
56	29304.5
57	23849.0
58	20775.5
59	17702.0
60	15628.5
61	13555.0
62	11591.0
63	9627.0
64	8294.0
65	6961.0
66	5915.0
67	4869.0
68	3875.5
69	2882.0
70	2506.0
71	2130.0
72	1756.5
73	1383.0
74	1200.5
75	797.5
76	577.0
77	474.0
78	371.0
79	278.0
80	185.0
81	158.5
82	132.0
83	86.0
84	40.0
85	29.5
86	19.0
87	10.5
88	2.0
89	3.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.81234537822674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65466430546644	31.961412723412046
2	9.611647563245654	8.229943502629286
3	3.907513066631786	5.0186939693572175
4	2.364086681221496	4.04848382002082
5	1.650367485617251	3.5328051397620706
6	1.280842007302092	3.2901510234935025
7	1.0408796354418377	3.1193748914789077
8	0.8572724609519371	2.9361475745213377
9	0.7059240154766435	2.7200036485233667
>10	3.860726302890686	25.094729625748506
>50	0.03898036699181448	1.1278922511791434
>100	0.01853944283743814	1.2988466883770526
>500	0.0033275923041555635	1.2123518838392515
>1k	0.004278332962485725	3.813301535587721
>5k	9.50740658330161E-4	2.5958617220697757
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	7565	1.5313579187297321	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	5190	1.0505945271919774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	4466	0.9040376027821524	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3650	0.7388574227843386	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2643	0.5350137447723307	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	2171	0.43946834653830114	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1334	0.27003720602583775	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1190	0.2408877624968118	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT	1126	0.22793245426168912	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT	1099	0.22246693359999678	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	1058	0.21416743926187132	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	963	0.19493690360036114	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCCAGGTT	958	0.19392477014449216	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	953	0.1929126366886232	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	889	0.17995732845350057	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	866	0.17530151455650336	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	665	0.1346137496305713	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTA	663	0.1342088962482237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20384367801200795	0.0
2	0.0	0.0	0.0	1.199378145204714	0.0
3	0.0	0.0	0.0	1.6999793524775002	0.0
4	0.0	0.0	0.0	2.6568503216560124	0.0
5	0.0	0.0	0.0	5.516937041250511	0.0
6	0.0	0.0	0.0	6.967931563584248	0.0
7	0.0	0.0	0.0	8.224191609008798	0.0
8	0.0	0.0	0.0	9.844617271854998	0.0
9	0.0	0.0	0.0	10.467686627287927	0.0
10	0.0	0.0	0.0	12.583045550054049	0.0
11	0.0	0.0	0.0	14.113593762019084	0.0
12	0.0	0.0	0.0	16.386440650518416	0.0
13	0.0	0.0	0.0	17.020845900657076	0.0
14	0.0	0.0	0.0	17.33177329830002	0.0
15	0.0	0.0	0.0	17.807678449249604	0.0
16	0.0	0.0	0.0	18.562730007327847	0.0
17	2.0242669117379142E-4	0.0	0.0	19.46150451613948	0.0
18	2.0242669117379142E-4	0.0	0.0	20.40177649664174	0.0
19	2.0242669117379142E-4	0.0	0.0	21.212900248175124	0.0
20	2.0242669117379142E-4	0.0	0.0	21.813297814196588	0.0
21	2.0242669117379142E-4	0.0	0.0	22.50478739124626	0.0
22	2.0242669117379142E-4	0.0	0.0	23.333522264911764	0.0
23	2.0242669117379142E-4	0.0	0.0	24.138775642401104	0.0
24	2.0242669117379142E-4	0.0	0.0	24.77561001283385	0.0
25	2.0242669117379142E-4	0.0	0.0	25.336331947385254	0.0
26	2.0242669117379142E-4	0.0	0.0	25.864260757966502	0.0
27	2.0242669117379142E-4	0.0	0.0	26.403525463253484	0.0
28	2.0242669117379142E-4	0.0	0.0	26.95756731699615	0.0
29	2.0242669117379142E-4	0.0	0.0	27.520515945150464	0.0
30	2.0242669117379142E-4	0.0	0.0	28.131439699112967	0.0
31	2.0242669117379142E-4	0.0	0.0	28.710177609178835	0.0
32	2.0242669117379142E-4	0.0	0.0	29.275960211009583	0.0
33	2.0242669117379142E-4	0.0	0.0	29.824536544090556	0.0
34	2.0242669117379142E-4	0.0	0.0	30.351453221215937	0.0
35	2.0242669117379142E-4	0.0	0.0	30.88019173856188	0.0
36	2.0242669117379142E-4	0.0	0.0	31.402452601790262	0.0
37	2.0242669117379142E-4	0.0	0.0	31.94859981457715	0.0
38	2.0242669117379142E-4	0.0	0.0	32.515192123172596	0.0
39	2.0242669117379142E-4	0.0	0.0	33.08522568551799	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.0293754E-4	45.000004	22
CCGATAC	20	7.0293754E-4	45.000004	10
GGCGTAA	20	7.0293754E-4	45.000004	8
AAACGGT	20	7.0293754E-4	45.000004	38
TCGGAGA	20	7.0293754E-4	45.000004	29
GTGTACG	20	7.0293754E-4	45.000004	1
ACCACGA	20	7.0293754E-4	45.000004	19
CGATCCC	20	7.0293754E-4	45.000004	34
ACGAACG	20	7.0293754E-4	45.000004	1
ATTACGG	25	3.8875653E-5	45.0	2
TTACGCG	25	3.8875653E-5	45.0	1
TACACGG	25	3.8875653E-5	45.0	2
CGCTCGA	30	2.1629803E-6	44.999996	41
CGTTTTT	2595	0.0	43.612717	1
TCAGACG	65	0.0	41.538464	22
GATAGCG	55	6.002665E-11	40.909092	1
CCGATGA	345	0.0	40.434784	18
TACGGCT	590	0.0	40.042374	7
CCGGGAT	45	1.9244908E-8	40.0	5
ACGGCTG	610	0.0	39.098362	8
>>END_MODULE