Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550857_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 671797 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC | 11634 | 1.7317731398026486 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC | 11244 | 1.6737198885972995 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG | 9936 | 1.4790182153239744 | No Hit |
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC | 4215 | 0.6274216764885822 | TruSeq Adapter, Index 19 (95% over 21bp) |
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT | 2507 | 0.3731782071072065 | No Hit |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT | 2211 | 0.32911727798724916 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT | 2171 | 0.3231630983764441 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC | 1755 | 0.26123963042407156 | No Hit |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA | 1738 | 0.25870910408947945 | No Hit |
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC | 1728 | 0.25722055918677816 | TruSeq Adapter, Index 19 (95% over 21bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1553 | 0.23117102338950607 | No Hit |
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT | 1173 | 0.1746063170868581 | TruSeq Adapter, Index 13 (95% over 22bp) |
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT | 875 | 0.13024767898636044 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGCGAA | 25 | 3.8890692E-5 | 45.0 | 31 |
GTACACG | 25 | 3.8890692E-5 | 45.0 | 1 |
CGTGCGG | 80 | 0.0 | 45.0 | 2 |
GCGAACC | 40 | 6.8084773E-9 | 45.0 | 33 |
TACGGGT | 35 | 1.2109194E-7 | 45.0 | 4 |
TAACGCG | 25 | 3.8890692E-5 | 45.0 | 1 |
TATTGCG | 20 | 7.0311903E-4 | 45.0 | 1 |
CCCGTTA | 20 | 7.0311903E-4 | 45.0 | 19 |
GGACGTA | 25 | 3.8890692E-5 | 45.0 | 8 |
CGATAGC | 20 | 7.0311903E-4 | 45.0 | 10 |
CCGTTAG | 20 | 7.0311903E-4 | 45.0 | 20 |
TAACGCC | 30 | 2.16415E-6 | 44.999996 | 12 |
ACGGCCT | 60 | 3.6379788E-12 | 41.249996 | 8 |
ACACGAC | 330 | 0.0 | 40.909092 | 26 |
ACGGCTG | 1335 | 0.0 | 40.78652 | 8 |
TACGGCT | 1335 | 0.0 | 40.617977 | 7 |
AAGCACG | 50 | 1.0804797E-9 | 40.5 | 1 |
CGGCTAT | 50 | 1.0804797E-9 | 40.5 | 31 |
CGATGAA | 590 | 0.0 | 40.42373 | 19 |
CCGATGA | 590 | 0.0 | 40.042374 | 18 |