FastQCFastQC Report
Sat 18 Jun 2016
SRR3550857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671797
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC116341.7317731398026486No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC112441.6737198885972995No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG99361.4790182153239744No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC42150.6274216764885822TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT25070.3731782071072065No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT22110.32911727798724916No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT21710.3231630983764441No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC17550.26123963042407156No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA17380.25870910408947945No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC17280.25722055918677816TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.23117102338950607No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT11730.1746063170868581TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT8750.13024767898636044No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA253.8890692E-545.031
GTACACG253.8890692E-545.01
CGTGCGG800.045.02
GCGAACC406.8084773E-945.033
TACGGGT351.2109194E-745.04
TAACGCG253.8890692E-545.01
TATTGCG207.0311903E-445.01
CCCGTTA207.0311903E-445.019
GGACGTA253.8890692E-545.08
CGATAGC207.0311903E-445.010
CCGTTAG207.0311903E-445.020
TAACGCC302.16415E-644.99999612
ACGGCCT603.6379788E-1241.2499968
ACACGAC3300.040.90909226
ACGGCTG13350.040.786528
TACGGCT13350.040.6179777
AAGCACG501.0804797E-940.51
CGGCTAT501.0804797E-940.531
CGATGAA5900.040.4237319
CCGATGA5900.040.04237418