SRR3550856_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381122
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2569	0.6740623737280975	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	2535	0.6651413458157754	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2223	0.5832777955615263	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1707	0.4478880778333447	RNA PCR Primer, Index 35 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1395	0.3660245275790954	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	775	0.2033469597661641	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	753	0.19757452994054397	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	545	0.14299882977104444	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT	543	0.1424740634232608	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	494	0.12961728790256138	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	464	0.12174579268580664	RNA PCR Primer, Index 35 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	412	0.10810186764343176	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	395	0.10364135368727075	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTTG	30	2.1616688E-6	45.000004	1
GGCTATC	30	2.1616688E-6	45.000004	8
TTATGCG	20	7.0273463E-4	45.0	1
TCGGGTA	20	7.0273463E-4	45.0	27
GCACGAA	20	7.0273463E-4	45.0	40
CCTCGTG	45	3.8380676E-10	45.0	15
TAACGCC	25	3.8858827E-5	45.0	12
ACGGGTA	20	7.0273463E-4	45.0	5
CCGTCCA	20	7.0273463E-4	45.0	28
ACGATGG	35	1.209064E-7	45.0	2
TCTACCG	20	7.0273463E-4	45.0	40
AAGCACG	20	7.0273463E-4	45.0	1
AACGATT	20	7.0273463E-4	45.0	23
CGTAAGG	35	1.209064E-7	45.0	2
CTCGAAT	20	7.0273463E-4	45.0	43
ATAACGC	25	3.8858827E-5	45.0	11
TATCGCG	20	7.0273463E-4	45.0	32
AGTACGG	20	7.0273463E-4	45.0	2
GCGTAAG	25	3.8858827E-5	45.0	1
GTTATAG	40	6.7939254E-9	45.0	1