##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381122
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41595604557071	31.0	31.0	33.0	28.0	34.0
2	31.339442488232116	31.0	31.0	34.0	28.0	34.0
3	31.26899260604216	31.0	31.0	34.0	28.0	34.0
4	35.104578061618064	37.0	35.0	37.0	32.0	37.0
5	35.373686116256735	37.0	35.0	37.0	33.0	37.0
6	35.299471035521435	37.0	35.0	37.0	33.0	37.0
7	35.6745950115711	37.0	35.0	37.0	35.0	37.0
8	35.3264702641149	37.0	35.0	37.0	33.0	37.0
9	37.2466191928044	39.0	37.0	39.0	34.0	39.0
10	36.67027880836058	38.0	35.0	39.0	32.0	39.0
11	36.78180740025503	39.0	37.0	39.0	33.0	39.0
12	36.83073923835412	39.0	35.0	39.0	33.0	39.0
13	36.82115175718012	39.0	35.0	39.0	33.0	39.0
14	37.781201295123346	40.0	37.0	41.0	33.0	41.0
15	37.920812758119446	40.0	37.0	41.0	33.0	41.0
16	37.93495258736048	40.0	37.0	41.0	33.0	41.0
17	37.80839731109723	39.0	36.0	41.0	33.0	41.0
18	37.80303157519115	39.0	36.0	41.0	33.0	41.0
19	37.67564192043493	39.0	36.0	41.0	33.0	41.0
20	37.751289613299676	39.0	36.0	41.0	33.0	41.0
21	37.658886130950194	39.0	36.0	41.0	33.0	41.0
22	37.82757752110873	39.0	36.0	41.0	33.0	41.0
23	37.901622052781	40.0	36.0	41.0	34.0	41.0
24	37.82228000482785	40.0	36.0	41.0	33.0	41.0
25	37.51327921243067	39.0	35.0	41.0	33.0	41.0
26	37.61350958485734	39.0	36.0	41.0	33.0	41.0
27	37.69428949260342	40.0	36.0	41.0	33.0	41.0
28	37.55422673054822	40.0	35.0	41.0	33.0	41.0
29	37.57915050823621	40.0	35.0	41.0	33.0	41.0
30	37.285336453943884	39.0	35.0	41.0	32.0	41.0
31	37.2069625999024	39.0	35.0	41.0	32.0	41.0
32	37.206283027482016	39.0	35.0	41.0	32.0	41.0
33	37.240935973257905	39.0	35.0	41.0	32.0	41.0
34	37.15120355161864	39.0	35.0	41.0	32.0	41.0
35	37.1529536473885	40.0	35.0	41.0	32.0	41.0
36	36.976702997990145	39.0	35.0	41.0	32.0	41.0
37	36.97592371996369	39.0	35.0	41.0	32.0	41.0
38	36.75734279312136	39.0	35.0	41.0	31.0	41.0
39	36.800439229433096	39.0	35.0	41.0	31.0	41.0
40	36.70962841294914	39.0	35.0	41.0	31.0	41.0
41	36.53355093644555	39.0	35.0	41.0	31.0	41.0
42	36.555289382402485	39.0	35.0	41.0	31.0	41.0
43	36.50381242751665	39.0	35.0	41.0	31.0	41.0
44	36.41560969978117	39.0	35.0	41.0	31.0	41.0
45	36.363332476214964	39.0	35.0	40.0	31.0	41.0
46	36.20761331017364	38.0	35.0	40.0	30.0	41.0
47	36.140091624204324	38.0	35.0	40.0	30.0	41.0
48	36.01961576608015	38.0	35.0	40.0	30.0	41.0
49	36.03037347620972	38.0	35.0	40.0	30.0	41.0
50	35.88080194793268	38.0	35.0	40.0	30.0	41.0
51	34.56043996410598	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	13.0
10	12.0
11	17.0
12	9.0
13	17.0
14	20.0
15	27.0
16	36.0
17	91.0
18	118.0
19	223.0
20	414.0
21	705.0
22	989.0
23	1327.0
24	1761.0
25	2307.0
26	2954.0
27	3528.0
28	4000.0
29	4857.0
30	6052.0
31	7866.0
32	10618.0
33	15337.0
34	28825.0
35	30083.0
36	30707.0
37	47704.0
38	83404.0
39	97074.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01157109796863	19.589265379589737	22.1356940822099	13.263469440231738
2	31.537145585927867	26.23018351079182	24.068670924270968	18.163999979009347
3	32.84617524047418	25.696233751921955	25.416008522205484	16.041582485398376
4	29.42994631640262	24.266245454211514	26.980861771296333	19.322946458089536
5	27.213858029712267	28.91226431431405	23.918587748804846	19.955289907168833
6	25.783082582480148	35.222842029586324	23.521864389880406	15.472210998053118
7	77.62501246320076	6.533603413080326	11.169651712574975	4.671732411143938
8	77.36079260709168	7.7885821338049235	9.206763188690235	5.643862070413149
9	71.78305109649928	7.9730375050508755	10.851643305818085	9.392268092631756
10	42.550679310037204	25.189834226310737	17.731067742087838	14.528418721564224
11	32.7973719701303	24.189891950608992	22.93858659431888	20.07414948494183
12	30.136019437345524	20.84896699744439	27.734163863539756	21.28084970167033
13	26.754425092227685	23.53262210000997	29.58553953852047	20.12741326924187
14	21.307350402233403	27.39516480287152	28.037478812558707	23.260005982336367
15	21.01007026621397	26.20394519340264	32.39382664868467	20.39215789169872
16	24.188055268391746	25.484490530591252	27.96479867339067	22.362655527626323
17	22.623202019300905	27.144326488630938	27.439245176085347	22.79322631598281
18	23.843808544245675	25.653990060925373	28.02068628942963	22.48151510539932
19	24.13374195139614	27.893168066918207	25.9066650573832	22.066424924302456
20	26.563410141634435	26.429332339775712	28.24895965071552	18.75829786787433
21	25.519649875892757	27.913371571307877	27.047769480638745	19.51920907216062
22	23.571454809745962	24.217704567041526	27.62186386511406	24.588976758098458
23	22.955641500621848	26.18820220296913	28.545190254039387	22.310966042369635
24	24.376184004072186	24.81121530638483	27.400412466349355	23.412188223193624
25	22.845965333935066	27.40198676539271	25.84789122643143	23.904156674240795
26	22.170066277989726	26.029460382764576	27.237997281710317	24.56247605753538
27	23.91937489832652	26.180855474100156	27.02966504164021	22.870104585933113
28	20.624367000592986	26.88273046426079	28.999113144872247	23.49378939027398
29	24.545421151232414	25.415746139031597	27.296508729488195	22.742323980247793
30	25.080682825971735	25.355660392210368	28.54361595499604	21.020040826821855
31	25.986954308594097	24.844275586295204	26.445337713383115	22.723432391727584
32	25.098262498622486	25.949958281075354	28.08234633529421	20.86943288500795
33	24.122721858092685	24.59527395427186	27.831245637879736	23.450758549755722
34	23.808386815770277	24.55749077723144	29.61807505208306	22.016047354915223
35	23.67352186438988	24.18831765156564	29.03689632191267	23.10126416213181
36	24.696816242567994	26.63609028080247	26.497551964987586	22.169541511641942
37	24.104355035920257	26.226772529531228	29.265169683198554	20.40370275134996
38	21.543495258736048	26.174295894752863	29.066020854214663	23.21618799229643
39	23.849056207723514	23.832526067768324	29.079140012909257	23.23927771159891
40	24.188842417913424	22.558655758523518	30.552946300659634	22.699555522903427
41	21.90742072092401	24.262572089777027	27.614254753071194	26.215752436227767
42	21.756812779110103	23.654367892695777	29.511547483482982	25.07727184471114
43	23.203855983123514	22.99473659353173	28.366507312619056	25.4349001107257
44	22.50434244152791	23.139047339172233	28.96684001448355	25.389770204816305
45	22.911823510581915	22.287613939893262	28.762968288369606	26.037594261155224
46	24.83692885742623	24.230298959388332	27.542361763424832	23.390410419760602
47	21.40626885879062	23.48959125949171	31.95039908480749	23.153740796910174
48	22.27134618311197	23.189949674907247	29.634605192038244	24.904098949942536
49	22.151961838991188	21.905846421880657	32.43580795650737	23.506383782620787
50	21.787773993629337	22.05724151321624	31.16928437613153	24.985700117022898
51	21.155168161376146	21.616175397904083	29.09567015286444	28.13298628785533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	461.0
2	743.0
3	1205.5
4	1668.0
5	1209.0
6	750.0
7	731.5
8	713.0
9	711.5
10	710.0
11	700.0
12	690.0
13	790.0
14	890.0
15	874.0
16	858.0
17	806.0
18	754.0
19	701.5
20	649.0
21	762.5
22	876.0
23	993.5
24	1111.0
25	1363.5
26	2051.0
27	2486.0
28	3160.0
29	3834.0
30	4691.0
31	5548.0
32	5898.5
33	6249.0
34	7203.0
35	8157.0
36	8711.5
37	9266.0
38	10462.5
39	11659.0
40	13293.5
41	14928.0
42	16100.0
43	17272.0
44	20445.5
45	23619.0
46	30996.5
47	38374.0
48	38306.0
49	38238.0
50	38068.0
51	37898.0
52	33938.0
53	29978.0
54	27348.5
55	24719.0
56	22626.5
57	20534.0
58	19555.5
59	18577.0
60	17298.0
61	16019.0
62	14439.5
63	12860.0
64	11362.5
65	9865.0
66	8282.0
67	6699.0
68	5565.5
69	4432.0
70	3744.5
71	3057.0
72	2483.5
73	1910.0
74	1580.0
75	1055.5
76	861.0
77	614.5
78	368.0
79	247.0
80	126.0
81	108.0
82	90.0
83	56.0
84	22.0
85	15.5
86	9.0
87	7.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	381122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.21506317123978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98609134016942	24.972237258257728
2	9.54220529594318	6.529743139872693
3	3.3734649351060306	3.462699475818453
4	1.8580991654097305	2.542999212916873
5	1.2897721903355672	2.2064818484419857
6	1.0150506269677935	2.0838012794225755
7	0.8768261227443601	2.1000462825924067
8	0.7759412483316053	2.123910306306922
9	0.7313018878430446	2.251938626081701
>10	7.438209264006898	44.36060154502865
>50	0.07458964996468852	1.825138706634353
>100	0.03152758400569309	2.1082364792073047
>500	0.003075861854213959	0.6882748820175101
>1k	0.0038448273177674494	2.743890957400846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2569	0.6740623737280975	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	2535	0.6651413458157754	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2223	0.5832777955615263	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1707	0.4478880778333447	RNA PCR Primer, Index 35 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1395	0.3660245275790954	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	775	0.2033469597661641	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	753	0.19757452994054397	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	545	0.14299882977104444	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT	543	0.1424740634232608	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	494	0.12961728790256138	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	464	0.12174579268580664	RNA PCR Primer, Index 35 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	412	0.10810186764343176	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	395	0.10364135368727075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21751565115632265	0.0
2	0.0	0.0	0.0	1.2607511505502176	0.0
3	0.0	0.0	0.0	1.5939777813928349	0.0
4	0.0	0.0	0.0	2.1155955310897823	0.0
5	0.0	0.0	0.0	3.89114246881576	0.0
6	0.0	0.0	0.0	4.650741757232592	0.0
7	0.0	0.0	0.0	5.499551324772645	0.0
8	0.0	0.0	0.0	6.642230047071541	0.0
9	0.0	0.0	0.0	7.0127150886067975	0.0
10	0.0	0.0	0.0	8.477862731618746	0.0
11	0.0	0.0	0.0	9.769837479862092	0.0
12	0.0	0.0	0.0	11.539611987762449	0.0
13	0.0	0.0	0.0	12.035516186417997	0.0
14	0.0	0.0	0.0	12.243586043314215	0.0
15	0.0	0.0	0.0	12.721123419797335	0.0
16	0.0	0.0	0.0	13.408304952220025	0.0
17	0.0	0.0	0.0	14.213558912894033	0.0
18	2.6238317389182464E-4	0.0	0.0	15.047412639522253	0.0
19	2.6238317389182464E-4	0.0	0.0	15.7487628633351	0.0
20	2.6238317389182464E-4	0.0	0.0	16.32836729446214	0.0
21	2.6238317389182464E-4	0.0	0.0	16.95913644449809	0.0
22	2.6238317389182464E-4	0.0	0.0	17.633723584573968	0.0
23	2.6238317389182464E-4	0.0	0.0	18.25662123939316	0.0
24	2.6238317389182464E-4	0.0	0.0	18.786110484306864	0.0
25	2.6238317389182464E-4	0.0	0.0	19.262335944920522	0.0
26	2.6238317389182464E-4	0.0	0.0	19.718357901144515	0.0
27	2.6238317389182464E-4	0.0	0.0	20.177003689107426	0.0
28	2.6238317389182464E-4	0.0	0.0	20.624104617419093	0.0
29	2.6238317389182464E-4	0.0	0.0	21.083275171729788	0.0
30	2.6238317389182464E-4	0.0	0.0	21.612502033469596	0.0
31	2.6238317389182464E-4	0.0	0.0	22.080855998866504	0.0
32	2.6238317389182464E-4	0.0	0.0	22.53766510461217	0.0
33	2.6238317389182464E-4	0.0	0.0	23.02648495757264	0.0
34	2.6238317389182464E-4	0.0	0.0	23.463877708450312	0.0
35	2.6238317389182464E-4	0.0	0.0	23.955846159497483	0.0
36	2.6238317389182464E-4	0.0	0.0	24.41239288206926	0.0
37	2.6238317389182464E-4	0.0	0.0	24.894653155682434	0.0
38	5.247663477836493E-4	0.0	0.0	25.39659216733749	0.0
39	5.247663477836493E-4	0.0	0.0	25.938938187771896	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTTG	30	2.1616688E-6	45.000004	1
GGCTATC	30	2.1616688E-6	45.000004	8
TTATGCG	20	7.0273463E-4	45.0	1
TCGGGTA	20	7.0273463E-4	45.0	27
GCACGAA	20	7.0273463E-4	45.0	40
CCTCGTG	45	3.8380676E-10	45.0	15
TAACGCC	25	3.8858827E-5	45.0	12
ACGGGTA	20	7.0273463E-4	45.0	5
CCGTCCA	20	7.0273463E-4	45.0	28
ACGATGG	35	1.209064E-7	45.0	2
TCTACCG	20	7.0273463E-4	45.0	40
AAGCACG	20	7.0273463E-4	45.0	1
AACGATT	20	7.0273463E-4	45.0	23
CGTAAGG	35	1.209064E-7	45.0	2
CTCGAAT	20	7.0273463E-4	45.0	43
ATAACGC	25	3.8858827E-5	45.0	11
TATCGCG	20	7.0273463E-4	45.0	32
AGTACGG	20	7.0273463E-4	45.0	2
GCGTAAG	25	3.8858827E-5	45.0	1
GTTATAG	40	6.7939254E-9	45.0	1
>>END_MODULE