SRR3550855_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593934
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	5635	0.9487586162772295	RNA PCR Primer, Index 34 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3614	0.6084851178750501	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	2828	0.47614718133664685	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	2536	0.426983469543754	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2013	0.3389265473941549	TruSeq Adapter, Index 18 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1785	0.3005384436654578	TruSeq Adapter, Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1188	0.20002222469163242	RNA PCR Primer, Index 34 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	1146	0.19295073189950399	RNA PCR Primer, Index 34 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT	1061	0.17863937743924407	RNA PCR Primer, Index 34 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	914	0.15388915266679465	RNA PCR Primer, Index 34 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	885	0.14900645526270595	TruSeq Adapter, Index 3 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT	772	0.12998077227436045	RNA PCR Primer, Index 34 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	728	0.12257254173022591	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	659	0.11095508928601494	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAT	20	7.030531E-4	45.0	27
CGACGGT	20	7.030531E-4	45.0	28
GCGAACG	20	7.030531E-4	45.0	1
AAGCGCG	20	7.030531E-4	45.0	1
CCGACAA	20	7.030531E-4	45.0	12
TATAGCG	35	1.2106102E-7	45.0	1
CGGTCTA	20	7.030531E-4	45.0	31
CGTTTTT	2175	0.0	42.51724	1
CGAACGG	40	3.454461E-7	39.375	2
ACACGAC	75	0.0	39.0	26
CACGACC	75	0.0	39.0	27
CCGATGA	180	0.0	38.75	18
ATTAGCG	35	6.243192E-6	38.571426	1
CGTAAGG	70	0.0	38.571426	2
ACGGGAT	390	0.0	37.5	5
ACAACGA	120	0.0	37.499996	13
GAATCGA	30	1.1392578E-4	37.499996	13
GACGGGT	30	1.1392578E-4	37.499996	4
TCGTAAG	30	1.1392578E-4	37.499996	1
ATAACGG	30	1.1392578E-4	37.499996	2