FastQCFastQC Report
Sat 18 Jun 2016
SRR3550852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475465
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC30060.6322231920330623No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC29220.6145562764872283TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG25990.5466227798050329No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC19120.40213264909088997TruSeq Adapter, Index 14 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC16020.3369333179098356TruSeq Adapter, Index 14 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.21389587035849114No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT8540.17961364138264646Illumina PCR Primer Index 3 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT5940.12493033135982669No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT5610.11798975739539187Illumina PCR Primer Index 3 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT5100.10726341581399261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA302.1628057E-645.00000411
GCGTTAA302.1628057E-645.00000412
GCGATAT302.1628057E-645.0000049
TTAGGCG253.887344E-545.01
ATTAGCG351.209919E-745.01
GTTACGG351.209919E-745.02
CTACGAA502.1827873E-1145.011
TACGAAT551.8189894E-1245.012
CGAATAT551.8189894E-1245.014
AGTTACG207.0291094E-445.01
TGTAGCG253.887344E-545.01
CGTCATA351.209919E-745.038
TGTCGCA207.0291094E-445.041
TGCGTAG253.887344E-545.01
CGCCAGT207.0291094E-445.026
CCCGATC207.0291094E-445.041
TTACGGG900.042.5000043
TACGGGA1550.042.096774
TCGTGTA750.042.00000417
CACTAGG700.041.7857132
Produced by FastQC (version 0.11.5)