##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475465
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.511265813466817	31.0	31.0	33.0	30.0	34.0
2	31.427055619235905	31.0	31.0	34.0	28.0	34.0
3	31.34106190781656	31.0	31.0	34.0	28.0	34.0
4	35.16612789584933	37.0	35.0	37.0	32.0	37.0
5	35.44913085085127	37.0	35.0	37.0	33.0	37.0
6	35.415504821595704	37.0	35.0	37.0	33.0	37.0
7	35.7794243530018	37.0	35.0	37.0	35.0	37.0
8	35.49220237031117	37.0	35.0	37.0	33.0	37.0
9	37.433815317636416	39.0	37.0	39.0	34.0	39.0
10	36.79570946336744	39.0	35.0	39.0	32.0	39.0
11	36.931820428422704	39.0	37.0	39.0	33.0	39.0
12	37.06939522362319	39.0	37.0	39.0	33.0	39.0
13	37.15135709253047	39.0	37.0	39.0	34.0	39.0
14	38.187149422144635	40.0	38.0	41.0	33.0	41.0
15	38.284952625324685	40.0	38.0	41.0	34.0	41.0
16	38.308096284689725	40.0	38.0	41.0	34.0	41.0
17	38.18840713827516	40.0	37.0	41.0	34.0	41.0
18	38.15927355325839	40.0	37.0	41.0	34.0	41.0
19	38.03201076840567	40.0	37.0	41.0	34.0	41.0
20	38.14714227125025	40.0	37.0	41.0	34.0	41.0
21	38.05518176942572	40.0	37.0	41.0	34.0	41.0
22	38.20552722072077	40.0	37.0	41.0	34.0	41.0
23	38.31209026952562	40.0	37.0	41.0	34.0	41.0
24	38.23856855919994	40.0	37.0	41.0	34.0	41.0
25	37.9477837485409	40.0	37.0	41.0	34.0	41.0
26	38.03406980534845	40.0	37.0	41.0	34.0	41.0
27	38.14640194336071	40.0	37.0	41.0	34.0	41.0
28	38.07235232877289	40.0	37.0	41.0	34.0	41.0
29	38.083023987044264	40.0	37.0	41.0	34.0	41.0
30	37.83809954465628	40.0	37.0	41.0	33.0	41.0
31	37.80293817631161	40.0	37.0	41.0	33.0	41.0
32	37.792161357828654	40.0	37.0	41.0	33.0	41.0
33	37.81227640310012	40.0	37.0	41.0	33.0	41.0
34	37.76387115770877	40.0	37.0	41.0	33.0	41.0
35	37.72964992165564	40.0	37.0	41.0	33.0	41.0
36	37.6374096936683	40.0	36.0	41.0	33.0	41.0
37	37.65122143585753	40.0	37.0	41.0	33.0	41.0
38	37.47865142544667	40.0	36.0	41.0	33.0	41.0
39	37.443971690871045	40.0	36.0	41.0	33.0	41.0
40	37.31785515232457	40.0	36.0	41.0	33.0	41.0
41	37.18097441452052	40.0	35.0	41.0	32.0	41.0
42	37.17313997875764	40.0	35.0	41.0	32.0	41.0
43	37.12497029224023	39.0	35.0	41.0	32.0	41.0
44	37.05011094402322	39.0	35.0	41.0	32.0	41.0
45	37.0006246516568	39.0	35.0	41.0	32.0	41.0
46	36.80081814644611	39.0	35.0	41.0	31.0	41.0
47	36.74267927187069	39.0	35.0	41.0	31.0	41.0
48	36.656233371541546	39.0	35.0	41.0	31.0	41.0
49	36.674459739412995	39.0	35.0	41.0	31.0	41.0
50	36.54262248535644	39.0	35.0	41.0	31.0	41.0
51	35.18580757784485	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	17.0
11	14.0
12	9.0
13	9.0
14	16.0
15	22.0
16	33.0
17	49.0
18	100.0
19	198.0
20	343.0
21	627.0
22	879.0
23	1159.0
24	1665.0
25	2215.0
26	2879.0
27	3585.0
28	4069.0
29	4823.0
30	6216.0
31	7944.0
32	10987.0
33	15697.0
34	28942.0
35	32970.0
36	36268.0
37	58520.0
38	110813.0
39	144353.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.603977159202046	20.741169171232375	23.120734438917694	13.534119230647892
2	32.07428517346177	26.554635987927604	23.81920856424763	17.55187027436299
3	31.774157929605757	25.26368923054273	26.311715899172388	16.650436940679125
4	28.879938586436438	24.8516715215631	27.363318014995848	18.905071877004616
5	26.733408347617594	29.644243004216925	24.432502918195873	19.18984572996961
6	25.86352307740843	35.624704236904925	23.376484073485955	15.135288612200688
7	79.90956221803918	5.427108199341697	10.079185639321505	4.584143943297613
8	80.30538525443514	6.579243477437877	8.10722135172936	5.008149916397632
9	74.31987633159119	7.448077145531216	10.072455385780236	8.159591137097367
10	37.61875216892936	32.16051654695929	17.23659996003912	12.984131324072223
11	27.505704941478342	24.704657545770985	28.352875605985716	19.436761906764957
12	25.51691502003302	22.4809397116507	30.930983353138508	21.071161915177775
13	24.134058237725174	24.073065315007412	32.59609014333337	19.196786303934044
14	19.90367324619057	27.78816526978852	30.35281250985877	21.955348974162135
15	19.579569474093784	27.14269189109608	33.517714237641044	19.760024397169087
16	22.12865300284984	27.007876499847516	29.45747846844668	21.405992028855962
17	21.399472095737856	27.001356566729413	28.954181695813574	22.64498964171916
18	22.19763810164786	26.146404046564943	30.130924463420023	21.525033388367177
19	22.033588171579403	27.896059646872008	27.912043999032527	22.158308182516063
20	24.01186207186649	28.09376084464682	29.474724743146187	18.41965234034051
21	24.38980787229344	27.953056481549645	28.697380459129484	18.959755187027437
22	21.832101206187627	24.72821343316543	29.60049635619867	23.839189004448276
23	21.172115718296823	27.266360299916926	29.69829535297025	21.863228628816
24	22.218039182694834	26.27007245538578	29.42572008454881	22.086168277370575
25	21.2633947819503	29.196891464145626	26.79986960133764	22.739844152566434
26	20.010095380311903	28.658681501267182	28.813477332716392	22.51774578570452
27	21.91286424868287	27.243225053368807	28.99267033325271	21.851240364695617
28	18.762895270945286	27.778700850746112	30.966317184230174	22.492086694078427
29	21.449948997297383	26.996519196996626	28.72955948387368	22.82397232183231
30	22.06892200267107	25.875721661951985	30.405813256496273	21.649543078880676
31	23.43411186943308	26.562207523161536	27.90994079480088	22.093739812604504
32	23.604261091773317	27.443450096221593	27.526106022525315	21.42618278947977
33	21.557212413111376	24.97660185292293	27.622222455911583	25.843963278054115
34	22.315627859043254	26.31423974425037	29.15125193231889	22.218880464387496
35	20.860631171589915	25.680964950101483	30.14522625219522	23.313177626113387
36	21.034986802393448	27.2796104865763	28.901811910445563	22.78359080058469
37	22.53520238082719	25.88623768311022	31.11227955790647	20.46628037815612
38	21.29830797219564	25.849221288633235	28.51061592335924	24.34185481581189
39	24.17864616743609	23.860221046764746	27.604134899519416	24.356997886279746
40	22.348227524633778	24.82117506020422	30.249124541238576	22.58147287392342
41	20.86715110470802	25.678861745869835	28.589486082045994	24.864501067376146
42	21.11364664065704	24.90004521889098	30.57533151756701	23.410976622884967
43	21.837569537189907	24.018382004984595	29.184903200025236	24.95914525780026
44	21.685718191665	24.75786861283165	28.88835140336302	24.668061792140325
45	22.28513139768437	23.033030822458013	28.385264951153083	26.29657282870453
46	23.974845677389503	24.565215105212793	28.227103992933234	23.23283522446447
47	20.407180339246842	24.936640972521637	32.14495283564511	22.511225852586417
48	20.710041748604	24.208932308371807	30.124614850725077	24.95641109229912
49	22.039056502581683	22.66160495514917	31.69991482022862	23.59942372204053
50	21.212917880390776	22.73521710325681	30.433785872777175	25.618079143575233
51	20.497197480361333	22.27419473567981	28.65279252941857	28.575815254540295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	457.0
2	724.0
3	1375.5
4	2027.0
5	1464.5
6	902.0
7	871.0
8	840.0
9	854.5
10	869.0
11	892.0
12	915.0
13	923.0
14	931.0
15	858.5
16	786.0
17	837.5
18	889.0
19	1003.0
20	1117.0
21	1270.0
22	1423.0
23	1870.0
24	2317.0
25	2385.0
26	3376.5
27	4300.0
28	5327.5
29	6355.0
30	7258.0
31	8161.0
32	9149.0
33	10137.0
34	12051.0
35	13965.0
36	14910.5
37	15856.0
38	18072.5
39	20289.0
40	21589.5
41	22890.0
42	25067.0
43	27244.0
44	29560.5
45	31877.0
46	41642.5
47	51408.0
48	50826.5
49	50245.0
50	48849.0
51	47453.0
52	40614.5
53	33776.0
54	29448.5
55	25121.0
56	22697.0
57	20273.0
58	18769.5
59	17266.0
60	16208.5
61	15151.0
62	13943.0
63	12735.0
64	10793.5
65	8852.0
66	7218.0
67	5584.0
68	4719.0
69	3854.0
70	3017.5
71	2181.0
72	1882.0
73	1583.0
74	1370.5
75	903.5
76	649.0
77	502.5
78	356.0
79	297.5
80	239.0
81	148.5
82	58.0
83	46.0
84	34.0
85	27.5
86	21.0
87	14.0
88	7.0
89	4.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.604623292032226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94619376151068	22.73832351128104
2	10.606648809214557	6.704382800122165
3	3.6562552908125774	3.4666371337689372
4	1.9287934535894509	2.4383516203532976
5	1.2024790296664838	1.9001948374588833
6	0.9310654512192306	1.7655583707605869
7	0.7262447273158849	1.6066883717250242
8	0.6088378271899411	1.5393672139422003
9	0.5516654718673722	1.569166147943055
>10	7.70563736272852	48.85789644321718
>50	0.09278850622087245	1.9894896943880733
>100	0.03671487656221572	2.137593805253442
>500	0.002670172840888416	0.5314448080767292
>1k	0.004005259261332624	2.754905241709381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGC	3006	0.6322231920330623	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2922	0.6145562764872283	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	2599	0.5466227798050329	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1912	0.40213264909088997	TruSeq Adapter, Index 14 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1602	0.3369333179098356	TruSeq Adapter, Index 14 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.21389587035849114	No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	854	0.17961364138264646	Illumina PCR Primer Index 3 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	594	0.12493033135982669	No Hit
GAACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	561	0.11798975739539187	Illumina PCR Primer Index 3 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTGCGTCT	510	0.10726341581399261	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18991934211771633	0.0
2	0.0	0.0	0.0	1.18578654580253	0.0
3	0.0	0.0	0.0	1.5378629341802235	0.0
4	0.0	0.0	0.0	2.108882883072361	0.0
5	0.0	0.0	0.0	4.0091279063653475	0.0
6	0.0	0.0	0.0	4.869969398378429	0.0
7	0.0	0.0	0.0	5.745743640436205	0.0
8	0.0	0.0	0.0	7.065293975371478	0.0
9	0.0	0.0	0.0	7.526316342948482	0.0
10	0.0	0.0	0.0	9.016226220647155	0.0
11	0.0	0.0	0.0	10.468699063022514	0.0
12	0.0	0.0	0.0	12.279557906470508	0.0
13	0.0	0.0	0.0	12.759719432555498	0.0
14	0.0	0.0	0.0	12.982869401533236	0.0
15	0.0	0.0	0.0	13.392573585858054	0.0
16	0.0	0.0	0.0	14.126171221856499	0.0
17	0.0	0.0	0.0	15.013723407611495	0.0
18	0.0	0.0	0.0	15.903168477174976	0.0
19	0.0	0.0	0.0	16.587761454576047	0.0
20	0.0	0.0	0.0	17.17602767816769	0.0
21	0.0	0.0	0.0	17.828231310401396	0.0
22	0.0	0.0	0.0	18.57886490067618	0.0
23	0.0	0.0	0.0	19.26745396611738	0.0
24	0.0	0.0	0.0	19.821227640310013	0.0
25	0.0	0.0	0.0	20.300758205125508	0.0
26	2.1032042316469142E-4	0.0	0.0	20.778606206555686	0.0
27	2.1032042316469142E-4	0.0	0.0	21.250144595290926	0.0
28	2.1032042316469142E-4	0.0	0.0	21.723365547411483	0.0
29	2.1032042316469142E-4	0.0	0.0	22.209416045345083	0.0
30	2.1032042316469142E-4	0.0	0.0	22.77959471254456	0.0
31	2.1032042316469142E-4	0.0	0.0	23.271954823173104	0.0
32	2.1032042316469142E-4	0.0	0.0	23.770624546496588	0.0
33	2.1032042316469142E-4	0.0	0.0	24.250996393004744	0.0
34	2.1032042316469142E-4	0.0	0.0	24.74440810574911	0.0
35	2.1032042316469142E-4	0.0	0.0	25.222045786756123	0.0
36	2.1032042316469142E-4	0.0	0.0	25.697369943108324	0.0
37	2.1032042316469142E-4	0.0	0.0	26.159023271954823	0.0
38	2.1032042316469142E-4	0.0	0.0	26.64717697412007	0.0
39	2.1032042316469142E-4	0.0	0.0	27.175291556686613	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	30	2.1628057E-6	45.000004	11
GCGTTAA	30	2.1628057E-6	45.000004	12
GCGATAT	30	2.1628057E-6	45.000004	9
TTAGGCG	25	3.887344E-5	45.0	1
ATTAGCG	35	1.209919E-7	45.0	1
GTTACGG	35	1.209919E-7	45.0	2
CTACGAA	50	2.1827873E-11	45.0	11
TACGAAT	55	1.8189894E-12	45.0	12
CGAATAT	55	1.8189894E-12	45.0	14
AGTTACG	20	7.0291094E-4	45.0	1
TGTAGCG	25	3.887344E-5	45.0	1
CGTCATA	35	1.209919E-7	45.0	38
TGTCGCA	20	7.0291094E-4	45.0	41
TGCGTAG	25	3.887344E-5	45.0	1
CGCCAGT	20	7.0291094E-4	45.0	26
CCCGATC	20	7.0291094E-4	45.0	41
TTACGGG	90	0.0	42.500004	3
TACGGGA	155	0.0	42.09677	4
TCGTGTA	75	0.0	42.000004	17
CACTAGG	70	0.0	41.785713	2
>>END_MODULE