FastQCFastQC Report
Sat 18 Jun 2016
SRR3550851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837741
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC45980.5488569856315973No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC41180.4915600406330835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG32660.3898579632607214No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC24120.28791714861753215No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.19480961299494712No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC12630.1507625865273396No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11170.1333347657569583No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT11080.13226044803823617No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT8720.10408945008063351No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA207.03219E-445.031
AAATCGT207.03219E-445.012
GGCCGTA207.03219E-445.028
CGAATAT1400.041.78571714
TACGGCT4750.041.210537
TACGTAG501.0804797E-940.51
GTGCAAG1800.040.01
CGGGTTA356.2462586E-638.571436
ATAGCGG1400.038.571432
CGACAGG950.037.8947372
TACGAAT1550.037.74193612
CGAGTGA301.1396578E-437.49999623
AATAGCG301.1396578E-437.4999961
CGTTTTT11500.037.1739161
AAGGGCG2800.036.9642875
CTACGAA1650.036.81818411
TTAAGCG552.746674E-936.818181
TATAGCG552.746674E-936.818181
ATAGGGA4600.036.684784
GCTACGA1600.036.562510