##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	837741
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41944228586162	31.0	31.0	33.0	28.0	34.0
2	31.34236357060237	31.0	31.0	34.0	28.0	34.0
3	31.281915293628938	31.0	31.0	34.0	28.0	34.0
4	35.10514466881769	37.0	35.0	37.0	32.0	37.0
5	35.39349035083636	37.0	35.0	37.0	33.0	37.0
6	35.32406077773441	37.0	35.0	37.0	33.0	37.0
7	35.721721868692114	37.0	35.0	37.0	35.0	37.0
8	35.42328595592194	37.0	35.0	37.0	33.0	37.0
9	37.36291049381611	39.0	37.0	39.0	34.0	39.0
10	36.68492768051223	38.0	35.0	39.0	32.0	39.0
11	36.82983523547254	39.0	37.0	39.0	33.0	39.0
12	36.975860080860315	39.0	37.0	39.0	33.0	39.0
13	37.070870352531394	39.0	37.0	39.0	33.0	39.0
14	38.09463545415588	40.0	37.0	41.0	33.0	41.0
15	38.23147846410764	40.0	38.0	41.0	34.0	41.0
16	38.248705745570525	40.0	37.0	41.0	34.0	41.0
17	38.12345223643107	40.0	37.0	41.0	33.0	41.0
18	38.10602202828798	40.0	37.0	41.0	34.0	41.0
19	37.96277369735992	40.0	37.0	41.0	34.0	41.0
20	38.10140604315654	40.0	37.0	41.0	34.0	41.0
21	37.96688714053627	40.0	37.0	41.0	33.0	41.0
22	38.14255121809724	40.0	37.0	41.0	34.0	41.0
23	38.21669227123896	40.0	37.0	41.0	34.0	41.0
24	38.12881785659291	40.0	37.0	41.0	34.0	41.0
25	37.794448403504184	40.0	36.0	41.0	33.0	41.0
26	37.91340282975287	40.0	37.0	41.0	33.0	41.0
27	38.00551841201517	40.0	37.0	41.0	34.0	41.0
28	37.93253284726425	40.0	37.0	41.0	33.0	41.0
29	37.935259226897095	40.0	37.0	41.0	34.0	41.0
30	37.67557037318217	40.0	36.0	41.0	33.0	41.0
31	37.64738982573373	40.0	36.0	41.0	33.0	41.0
32	37.65013172328918	40.0	36.0	41.0	33.0	41.0
33	37.6558960346933	40.0	36.0	41.0	33.0	41.0
34	37.631437401297056	40.0	36.0	41.0	33.0	41.0
35	37.62763312288643	40.0	36.0	41.0	33.0	41.0
36	37.52407844429245	40.0	36.0	41.0	33.0	41.0
37	37.565401478499915	40.0	36.0	41.0	33.0	41.0
38	37.375970616216705	40.0	36.0	41.0	33.0	41.0
39	37.36759571275609	40.0	36.0	41.0	33.0	41.0
40	37.24476299954282	40.0	35.0	41.0	32.0	41.0
41	37.09560234010273	39.0	35.0	41.0	32.0	41.0
42	37.155716384897005	39.0	35.0	41.0	32.0	41.0
43	37.117531552114556	39.0	35.0	41.0	32.0	41.0
44	37.055827517096574	39.0	35.0	41.0	32.0	41.0
45	36.987061633607524	39.0	35.0	41.0	32.0	41.0
46	36.87433586275472	39.0	35.0	41.0	32.0	41.0
47	36.85287696316642	39.0	35.0	41.0	32.0	41.0
48	36.75955217662738	39.0	35.0	41.0	32.0	41.0
49	36.74965771043795	39.0	35.0	41.0	32.0	41.0
50	36.65292494935785	39.0	35.0	41.0	31.0	41.0
51	35.49419570010301	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	21.0
9	36.0
10	41.0
11	38.0
12	22.0
13	28.0
14	37.0
15	48.0
16	69.0
17	126.0
18	229.0
19	404.0
20	662.0
21	1111.0
22	1626.0
23	2254.0
24	3121.0
25	4289.0
26	5330.0
27	6326.0
28	7387.0
29	8775.0
30	11135.0
31	14665.0
32	19998.0
33	28379.0
34	51824.0
35	57494.0
36	65701.0
37	104371.0
38	192798.0
39	249355.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4884027402264	19.67481596340635	22.366936797888606	12.469844498478647
2	31.77294653120714	25.680610116969326	24.568094434914848	17.978348916908686
3	33.57159312961882	24.900058609999988	25.466701522308206	16.061646738072984
4	29.02257380264306	25.494036939817917	26.160949505873532	19.32243975166549
5	27.103006776557432	29.77734168436307	23.119317306900342	20.000334232179156
6	26.446479281782793	36.48979815957438	22.43414133962645	14.62958121901638
7	80.12345104274472	6.03539757514554	9.307888715008577	4.533262667101169
8	80.8656852177463	6.575301913121121	7.6818491634049195	4.877163705727665
9	74.30315574861443	7.928703501440183	9.884558592691537	7.883582157253853
10	40.06608247656495	28.301706613380507	17.292576106457723	14.339634803596816
11	29.597811256701057	25.690040239167	25.185588385909245	19.526560118222697
12	27.737689811051386	22.665597123693363	28.387771399513696	21.20894166574156
13	25.24372091135566	23.67843999517751	30.261739606871334	20.816099486595498
14	20.778140260533984	28.198572112383186	28.174698385300466	22.848589241782367
15	19.98589062729412	27.478063029026874	32.090944575948896	20.445101767730122
16	22.842143335470034	26.321977795046443	29.328634983843454	21.507243885640072
17	21.978391889617434	27.280985411959062	27.847747692902697	22.8928750055208
18	23.29813152274987	26.251311562881607	28.26804465819388	22.18251225617464
19	23.729768508405343	27.954343884327017	26.29882027977621	22.017067327491432
20	26.39980614533609	25.965901155607757	27.796419179674864	19.83787351938129
21	24.835360809605834	27.889526715297446	27.27883677652162	19.996275698575097
22	23.36366490359192	24.519511400301525	27.742703293738757	24.374120402367797
23	22.860287368052894	27.54813241801464	27.88713934258918	21.70444087134329
24	22.884161095135607	25.39090243882059	28.69335510617243	23.031581359871367
25	22.487141013750072	28.52170300844772	25.96757231650355	23.02358366129866
26	22.016231747043538	28.07980032014668	26.77665292733673	23.12731500547305
27	23.047457388381375	27.52115510641117	26.856868650334647	22.574518854872807
28	20.56769335629986	29.00025186782072	27.72348494343717	22.708569832442247
29	21.998087714460674	27.532137020869218	26.754092255243567	23.71568300942654
30	22.19862702195547	26.98435435295634	27.26272201074079	23.554296614347393
31	22.594572785622287	28.93638964787446	24.570601176258535	23.89843639024472
32	22.559359038175284	29.194225900367776	24.93563046335323	23.31078459810371
33	21.59044382452333	28.4511561449183	25.008564699590924	24.949835330967446
34	20.460500321698472	30.08734203053211	26.546271460988542	22.90588618678088
35	20.91780156396786	27.081401053547577	25.630355921460215	26.370441461024352
36	20.444027450011397	31.096126368412197	25.634891929605928	22.82495425197048
37	21.086708183078063	28.978049301633796	26.207145167778584	23.728097347509554
38	20.382791340044236	28.97267771304019	25.684907387844213	24.95962355907136
39	22.18943563702863	26.529559852030637	26.51189329398943	24.769111216951302
40	22.09895421138514	26.668982418193686	28.18353166432107	23.048531706100096
41	19.2873453728539	28.093766450490065	27.90767074788031	24.71121742877572
42	20.794493763585642	27.36872135898804	27.65257997400151	24.184204903424806
43	21.466658549599458	26.35456543251434	28.019161053356584	24.15961496452961
44	22.07543859020867	26.9065260026667	26.697511522057532	24.320523885067104
45	21.471910769557656	25.555153681149662	26.522158996635	26.450776552657686
46	22.331006838629122	27.439745697059116	25.60075249987765	24.628494964434115
47	20.750685474388863	26.97086569715461	28.872408059292788	23.406040769163738
48	20.64396991432913	26.40661015755466	27.923188670484077	25.02623125763213
49	20.58273380436197	25.879955738109988	29.028780971684565	24.50852948584348
50	20.05488569856316	25.685265493750453	29.007652723216363	25.252196084470018
51	19.98803926273156	24.91486032079127	27.47686934267273	27.620231073804437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	703.0
2	1130.0
3	2221.5
4	3313.0
5	2341.0
6	1369.0
7	1341.0
8	1313.0
9	1367.5
10	1422.0
11	1402.5
12	1383.0
13	1360.5
14	1338.0
15	1363.5
16	1389.0
17	1497.5
18	1606.0
19	1638.5
20	1671.0
21	1992.5
22	2314.0
23	2647.0
24	2980.0
25	3494.5
26	5218.5
27	6428.0
28	7936.0
29	9444.0
30	11192.0
31	12940.0
32	14475.5
33	16011.0
34	19595.5
35	23180.0
36	24727.0
37	26274.0
38	28755.0
39	31236.0
40	36014.0
41	40792.0
42	44870.0
43	48948.0
44	63189.5
45	77431.0
46	76998.5
47	76566.0
48	77651.5
49	78737.0
50	71491.5
51	64246.0
52	59300.0
53	54354.0
54	50803.5
55	47253.0
56	44565.0
57	41877.0
58	40042.0
59	38207.0
60	37094.0
61	35981.0
62	31971.5
63	27962.0
64	24014.5
65	20067.0
66	16482.0
67	12897.0
68	10464.5
69	8032.0
70	6814.5
71	5597.0
72	4547.5
73	3498.0
74	2856.0
75	1731.5
76	1249.0
77	815.5
78	382.0
79	291.5
80	201.0
81	143.5
82	86.0
83	96.5
84	107.0
85	62.0
86	17.0
87	12.5
88	8.0
89	4.5
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	837741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.01210279066191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61861308498925	20.778065645463524
2	9.908672164859846	5.749428307317686
3	3.579326860218232	3.1153139637008556
4	1.8217075063251245	2.1140626171209953
5	1.2210074050268267	1.771199617139883
6	0.8736246720436233	1.520741327147274
7	0.7759037182750924	1.575741890117763
8	0.6807936247469552	1.580100369630878
9	0.5850719109840657	1.5276749779258838
>10	8.766658482640567	52.61091350887993
>50	0.11117385642338673	2.1435037013732434
>100	0.05166071354955673	2.684625360930324
>500	0.002479714250378723	0.48884401949588796
>1k	0.0033062856671716304	2.3397846937558886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	4598	0.5488569856315973	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC	4118	0.4915600406330835	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	3266	0.3898579632607214	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2412	0.28791714861753215	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1632	0.19480961299494712	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1263	0.1507625865273396	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1117	0.1333347657569583	No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1108	0.13226044803823617	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	872	0.10408945008063351	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3873727082714108E-4	0.0	0.0	0.10743177187221349	0.0
2	2.3873727082714108E-4	0.0	0.0	0.6597504479308044	0.0
3	2.3873727082714108E-4	0.0	0.0	0.8855959061332799	0.0
4	2.3873727082714108E-4	0.0	0.0	1.3073252950494245	0.0
5	2.3873727082714108E-4	0.0	0.0	2.7017896939507557	0.0
6	2.3873727082714108E-4	0.0	0.0	3.484489836357538	0.0
7	2.3873727082714108E-4	0.0	0.0	4.146866394267441	0.0
8	2.3873727082714108E-4	0.0	0.0	5.133686903231428	0.0
9	2.3873727082714108E-4	0.0	0.0	5.487495538597251	0.0
10	3.5810590624071165E-4	0.0	0.0	6.597265742037217	0.0
11	3.5810590624071165E-4	0.0	0.0	7.6286107520104665	0.0
12	3.5810590624071165E-4	0.0	0.0	8.977715069454641	0.0
13	3.5810590624071165E-4	0.0	0.0	9.356591118257313	0.0
14	3.5810590624071165E-4	0.0	0.0	9.540060710887971	0.0
15	4.7747454165428217E-4	0.0	0.0	9.872144254608525	0.0
16	4.7747454165428217E-4	0.0	0.0	10.422314295229672	1.1936863541357054E-4
17	5.968431770678527E-4	0.0	0.0	11.088271912201982	1.1936863541357054E-4
18	5.968431770678527E-4	0.0	0.0	11.735249916143534	1.1936863541357054E-4
19	7.162118124814233E-4	0.0	0.0	12.271573195056707	1.1936863541357054E-4
20	7.162118124814233E-4	0.0	0.0	12.708104294764134	1.1936863541357054E-4
21	7.162118124814233E-4	0.0	0.0	13.24454694231272	1.1936863541357054E-4
22	7.162118124814233E-4	0.0	0.0	13.827065883130944	1.1936863541357054E-4
23	7.162118124814233E-4	0.0	0.0	14.383681830064424	1.1936863541357054E-4
24	7.162118124814233E-4	0.0	0.0	14.833462848302757	1.1936863541357054E-4
25	7.162118124814233E-4	0.0	0.0	15.233228408302805	1.1936863541357054E-4
26	7.162118124814233E-4	0.0	0.0	15.606971605782695	1.1936863541357054E-4
27	7.162118124814233E-4	0.0	0.0	15.987399446845744	1.1936863541357054E-4
28	7.162118124814233E-4	0.0	0.0	16.377615516012707	1.1936863541357054E-4
29	7.162118124814233E-4	0.0	0.0	16.801254803095468	1.1936863541357054E-4
30	7.162118124814233E-4	0.0	0.0	17.285294619697495	1.1936863541357054E-4
31	8.355804478949938E-4	0.0	0.0	17.720990138957028	1.1936863541357054E-4
32	8.355804478949938E-4	0.0	0.0	18.16802567858085	1.1936863541357054E-4
33	8.355804478949938E-4	0.0	0.0	18.59894645242384	1.1936863541357054E-4
34	9.549490833085643E-4	0.0	0.0	19.001218753767574	1.1936863541357054E-4
35	9.549490833085643E-4	0.0	0.0	19.439898488912444	1.1936863541357054E-4
36	9.549490833085643E-4	0.0	0.0	19.871893580474158	1.1936863541357054E-4
37	0.001074317718722135	0.0	0.0	20.310095841077374	1.1936863541357054E-4
38	0.001074317718722135	0.0	0.0	20.754863376628336	1.1936863541357054E-4
39	0.001074317718722135	0.0	0.0	21.1907976331587	1.1936863541357054E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.03219E-4	45.0	31
AAATCGT	20	7.03219E-4	45.0	12
GGCCGTA	20	7.03219E-4	45.0	28
CGAATAT	140	0.0	41.785717	14
TACGGCT	475	0.0	41.21053	7
TACGTAG	50	1.0804797E-9	40.5	1
GTGCAAG	180	0.0	40.0	1
CGGGTTA	35	6.2462586E-6	38.57143	6
ATAGCGG	140	0.0	38.57143	2
CGACAGG	95	0.0	37.894737	2
TACGAAT	155	0.0	37.741936	12
CGAGTGA	30	1.1396578E-4	37.499996	23
AATAGCG	30	1.1396578E-4	37.499996	1
CGTTTTT	1150	0.0	37.173916	1
AAGGGCG	280	0.0	36.964287	5
CTACGAA	165	0.0	36.818184	11
TTAAGCG	55	2.746674E-9	36.81818	1
TATAGCG	55	2.746674E-9	36.81818	1
ATAGGGA	460	0.0	36.68478	4
GCTACGA	160	0.0	36.5625	10
>>END_MODULE