FastQCFastQC Report
Sat 18 Jun 2016
SRR3550846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602399
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC58540.9717811616553149No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC35890.5957845215546507No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG30290.5028228798520582No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.47410437268322164No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC24010.3985730387998652No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT17500.29050513032060143No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC13790.22891804269263394No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT10080.16733095506466644No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG8930.14824061792931262No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT8250.13695241857971213No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT8090.13429637167392375No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT7570.12566421923011162No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC6890.1143760198805111No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT6720.11155397004311096No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT6310.1047478498470283No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG207.0306094E-445.01
ACGGCGC207.0306094E-445.08
CGCGGAT253.8885883E-544.9999964
GTATGCG253.8885883E-544.9999961
TCGTCGG253.8885883E-544.9999962
CGTTTTT18600.042.943551
CGAAAGG1050.042.8571432
CGGGACG700.041.7857176
ATTCGGA650.041.5384610
GATACGT403.45457E-739.3759
GCGCGAC403.45457E-739.3759
CATACGA800.039.37518
GCGCTAG403.45457E-739.3751
CGAACGG356.2433373E-638.571432
GCGAACG356.2433373E-638.571431
GTTAGCG356.2433373E-638.571431
AATGCGG356.2433373E-638.571432
TACGCGG356.2433373E-638.571432
TTAAGCG356.2433373E-638.571431
GCTAGCG356.2433373E-638.571431
Produced by FastQC (version 0.11.5)