##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602399
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42470854035282	31.0	31.0	33.0	28.0	34.0
2	31.351217382498977	31.0	31.0	34.0	28.0	34.0
3	31.230060142862122	31.0	31.0	34.0	28.0	34.0
4	35.06936432497398	37.0	35.0	37.0	32.0	37.0
5	35.37862778656671	37.0	35.0	37.0	33.0	37.0
6	35.32359615470809	37.0	35.0	37.0	33.0	37.0
7	35.68945665580454	37.0	35.0	37.0	35.0	37.0
8	35.476813540527125	37.0	35.0	37.0	33.0	37.0
9	37.43514182460462	39.0	37.0	39.0	34.0	39.0
10	36.67146027798851	38.0	35.0	39.0	32.0	39.0
11	36.87170629433316	39.0	37.0	39.0	33.0	39.0
12	37.080041633535245	39.0	37.0	39.0	34.0	39.0
13	37.19658565170261	39.0	37.0	39.0	34.0	39.0
14	38.25788389422957	40.0	38.0	41.0	34.0	41.0
15	38.347912264130585	40.0	38.0	41.0	34.0	41.0
16	38.35585716443752	40.0	38.0	41.0	34.0	41.0
17	38.22762985994333	40.0	37.0	41.0	34.0	41.0
18	38.18166364818003	40.0	37.0	41.0	34.0	41.0
19	38.01686091776381	40.0	37.0	41.0	34.0	41.0
20	38.095056598699536	40.0	37.0	41.0	34.0	41.0
21	38.01829850315156	40.0	37.0	41.0	34.0	41.0
22	38.19080874968252	40.0	37.0	41.0	34.0	41.0
23	38.2751896998501	40.0	37.0	41.0	34.0	41.0
24	38.19024931980299	40.0	37.0	41.0	34.0	41.0
25	37.88620665040945	40.0	37.0	41.0	33.0	41.0
26	37.99312581860195	40.0	37.0	41.0	34.0	41.0
27	38.1117996543819	40.0	37.0	41.0	34.0	41.0
28	38.02962488317544	40.0	37.0	41.0	34.0	41.0
29	38.077699332170205	40.0	37.0	41.0	34.0	41.0
30	37.805808110571235	40.0	37.0	41.0	33.0	41.0
31	37.75819680975566	40.0	37.0	41.0	33.0	41.0
32	37.68825313455036	40.0	37.0	41.0	33.0	41.0
33	37.643038916067255	40.0	37.0	41.0	33.0	41.0
34	37.532054336079575	40.0	37.0	41.0	33.0	41.0
35	37.430618244718204	40.0	37.0	41.0	33.0	41.0
36	37.3556280803919	40.0	37.0	41.0	33.0	41.0
37	37.39180011919011	40.0	37.0	41.0	33.0	41.0
38	37.23859933366423	40.0	36.0	41.0	32.0	41.0
39	37.216130836870576	40.0	36.0	41.0	32.0	41.0
40	37.102188084641575	40.0	36.0	41.0	32.0	41.0
41	36.96348101507473	40.0	36.0	41.0	31.0	41.0
42	36.99527555656633	40.0	36.0	41.0	31.0	41.0
43	37.00487882616007	40.0	36.0	41.0	32.0	41.0
44	36.97046475840764	40.0	36.0	41.0	32.0	41.0
45	36.94059087083478	40.0	36.0	41.0	31.0	41.0
46	36.803616871873956	39.0	35.0	41.0	31.0	41.0
47	36.76874463603027	39.0	35.0	41.0	31.0	41.0
48	36.67306718636651	39.0	35.0	41.0	31.0	41.0
49	36.68896528712697	39.0	35.0	41.0	31.0	41.0
50	36.602222115242554	39.0	35.0	41.0	31.0	41.0
51	35.4474575821009	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	10.0
10	15.0
11	16.0
12	11.0
13	13.0
14	16.0
15	33.0
16	41.0
17	57.0
18	137.0
19	232.0
20	496.0
21	819.0
22	1326.0
23	1815.0
24	2717.0
25	3947.0
26	5359.0
27	5812.0
28	5973.0
29	6439.0
30	7669.0
31	10191.0
32	13509.0
33	19183.0
34	32675.0
35	38147.0
36	46026.0
37	72041.0
38	140605.0
39	187036.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9547542409599	20.24306149246596	22.30780595585318	13.494378310720966
2	33.00271082787322	25.90641750733318	24.59233830069439	16.49853336409921
3	30.371066353031793	25.588853899159858	28.033911078869657	16.006168668938695
4	27.411068079462282	24.78805575706467	29.307153564331944	18.4937225991411
5	25.545361131077577	29.109112066919103	26.252367616812112	19.09315918519121
6	24.453393846935338	35.62489313561277	25.9334759851859	13.98823703226599
7	78.72108021427658	5.068069502107407	11.976945512857757	4.233904770758252
8	79.7253979505278	5.897420148439822	9.64775837941298	4.729423521619392
9	73.83743996918986	6.957514869712599	11.265622950901314	7.9394222101962315
10	35.919880345086895	29.284909171495972	20.216501023408075	14.578709460009065
11	26.198748669901512	24.748547059341067	28.91787668970234	20.13482758105508
12	23.58171245304192	22.02576697504478	32.816787544468035	21.575733027445267
13	22.854619612582358	23.212687936068953	35.04554290428769	18.887149547061004
14	20.167032149787765	27.977636085053266	31.19128683812556	20.664044927033412
15	18.95155868452637	28.404761628090352	34.21967831951912	18.424001367864157
16	21.51530796033858	26.852966223383508	30.515488903534038	21.11623691274388
17	20.770120800333334	27.808977106535703	29.96950526146292	21.451396831668045
18	21.35793718117062	26.947587894402215	30.565621788880794	21.128853135546375
19	21.131509182452163	28.806322719659228	28.651275981533836	21.410892116354773
20	22.488915154241624	28.24954888703334	30.391650716551656	18.869885242173375
21	22.453390526876703	28.553168248951277	30.558483662821484	18.434957561350533
22	20.68263725537393	25.027763990312067	30.477806238058164	23.811792516255835
23	20.1026230123224	27.768970400017263	30.505860733500555	21.62254585415978
24	21.0460176726721	26.43862290608052	30.424519297010782	22.090840124236593
25	20.43612290193045	29.90758616797173	28.252868945665583	21.403421984432246
26	19.221811457190334	28.09500015770279	30.030262334432827	22.652926050674054
27	21.18961020851628	27.2448991449189	30.309479265403827	21.25601138116099
28	18.71118643955252	27.24589516250857	32.30649453269345	21.73642386524546
29	20.198738709725614	24.980121148939492	31.826580057403813	22.99456008393108
30	20.494555933857793	25.88749317312944	32.26681983203824	21.351131060974538
31	21.033567452801215	26.070262400834	30.223987755623767	22.672182390741018
32	19.559793425951902	27.87039819123206	29.672194010946235	22.897614371869807
33	19.746214718151922	26.36259356340233	28.766648019004016	25.12454369944173
34	20.79950331922862	25.92451182687886	30.125714020109594	23.150270833782923
35	19.377190201178955	27.216014634818453	29.804332344509206	23.602462819493393
36	19.49887035005038	28.932484947684177	30.08321726961698	21.48542743264846
37	19.224799509959347	28.025610932289062	31.158418257666433	21.59117130008516
38	18.836186647056188	28.198253981165305	28.652438002055113	24.31312136972339
39	21.877692360047078	25.89147724348812	27.807981088946033	24.42284930751877
40	21.38715369713429	26.128695432761344	29.82624473148196	22.65790613862241
41	19.820584031513995	28.4195358890038	29.20008167344235	22.55979840603985
42	19.627522622049508	28.105624345325936	30.605296489536006	21.66155654308855
43	20.556641030280595	27.096492524057975	29.689126309970632	22.657740135690794
44	20.246049545234968	25.03739216034555	29.824750705097454	24.891807589322028
45	20.716999862217566	23.732609117877022	29.071927410238064	26.478463609667347
46	22.80880280345751	25.70870801578356	28.258679048271993	23.22381013248694
47	19.299002820389806	25.57374763238319	32.3805318401923	22.746717707034705
48	19.200231076080804	25.903263451632558	30.418543191472764	24.477962280813877
49	19.42466703961992	24.943268498121675	31.98942893331496	23.64263552894344
50	19.558963411293842	23.753691490191716	31.43481313880003	25.252531959714407
51	19.548007217807466	23.18064937026788	29.28324914217985	27.9880942697448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	308.5
2	488.0
3	2669.5
4	4851.0
5	3419.0
6	1987.0
7	1912.5
8	1838.0
9	1860.0
10	1882.0
11	1845.0
12	1808.0
13	1811.0
14	1814.0
15	1768.0
16	1722.0
17	1721.0
18	1720.0
19	1750.0
20	1780.0
21	1976.5
22	2173.0
23	2550.5
24	2928.0
25	3298.0
26	4196.5
27	4725.0
28	5985.5
29	7246.0
30	8630.0
31	10014.0
32	11957.5
33	13901.0
34	16134.5
35	18368.0
36	19938.5
37	21509.0
38	23804.0
39	26099.0
40	28867.0
41	31635.0
42	35786.0
43	39937.0
44	54914.0
45	69891.0
46	67773.0
47	65655.0
48	65339.5
49	65024.0
50	56177.0
51	47330.0
52	41837.0
53	36344.0
54	31687.0
55	27030.0
56	24857.5
57	22685.0
58	20527.0
59	18369.0
60	16913.5
61	15458.0
62	14167.0
63	12876.0
64	10409.5
65	7943.0
66	6404.0
67	4865.0
68	3988.5
69	3112.0
70	2297.5
71	1483.0
72	1252.5
73	1022.0
74	785.5
75	419.5
76	290.0
77	224.0
78	158.0
79	100.0
80	42.0
81	30.0
82	18.0
83	21.0
84	24.0
85	14.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.02586524221636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39263284931351	27.908183664994578
2	9.87372110057104	7.50913576019085
3	3.7853605666185492	4.318248323983101
4	2.190164586989408	3.3313161377253464
5	1.4879337709773075	2.8289984532262955
6	1.161007490172267	2.648898861989667
7	0.9349877802508257	2.488760353445583
8	0.8056182004490379	2.450746330156157
9	0.7217243893297156	2.46997749336054
>10	5.550611505932401	35.99062311806284
>50	0.06167965489246878	1.6012081226945836
>100	0.027121550369815294	1.7555735012324685
>500	0.003936999247231252	1.0610956380862153
>1k	0.003062110525624307	2.6634681544186365
>5k	4.374443608034725E-4	0.9737660864330937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	5854	0.9717811616553149	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	3589	0.5957845215546507	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	3029	0.5028228798520582	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2856	0.47410437268322164	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2401	0.3985730387998652	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	1750	0.29050513032060143	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1379	0.22891804269263394	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1008	0.16733095506466644	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	893	0.14824061792931262	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	825	0.13695241857971213	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	809	0.13429637167392375	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	757	0.12566421923011162	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC	689	0.1143760198805111	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT	672	0.11155397004311096	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	631	0.1047478498470283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6600293161177226E-4	0.0	0.0	0.1736390664659138	0.0
2	1.6600293161177226E-4	0.0	0.0	0.9654730502540675	0.0
3	1.6600293161177226E-4	0.0	0.0	1.359564009900415	0.0
4	1.6600293161177226E-4	0.0	0.0	2.1168693839133197	0.0
5	1.6600293161177226E-4	0.0	0.0	4.320226295196373	0.0
6	1.6600293161177226E-4	0.0	0.0	5.602598941897314	0.0
7	1.6600293161177226E-4	0.0	0.0	6.614386810071066	0.0
8	1.6600293161177226E-4	0.0	0.0	8.026739752224024	0.0
9	1.6600293161177226E-4	0.0	0.0	8.556289104065577	0.0
10	1.6600293161177226E-4	0.0	0.0	10.129664889881955	0.0
11	1.6600293161177226E-4	0.0	0.0	11.609249019337682	0.0
12	1.6600293161177226E-4	0.0	0.0	13.373196170644373	0.0
13	1.6600293161177226E-4	0.0	0.0	13.943748246594035	0.0
14	1.6600293161177226E-4	0.0	0.0	14.221803157043754	0.0
15	1.6600293161177226E-4	0.0	0.0	14.637972506594467	0.0
16	1.6600293161177226E-4	0.0	0.0	15.316924496886616	0.0
17	1.6600293161177226E-4	0.0	0.0	16.228280591435244	0.0
18	1.6600293161177226E-4	0.0	0.0	17.17466330455396	0.0
19	1.6600293161177226E-4	0.0	0.0	17.8929579896381	0.0
20	3.3200586322354453E-4	0.0	0.0	18.494884619662383	0.0
21	3.3200586322354453E-4	0.0	0.0	19.205875175755605	0.0
22	3.3200586322354453E-4	0.0	0.0	20.016301487884277	0.0
23	3.3200586322354453E-4	0.0	0.0	20.817929644637523	0.0
24	3.3200586322354453E-4	0.0	0.0	21.432472497464307	0.0
25	3.3200586322354453E-4	0.0	0.0	21.97895414833026	0.0
26	3.3200586322354453E-4	0.0	0.0	22.47181685228561	0.0
27	3.3200586322354453E-4	0.0	0.0	23.003192236374893	0.0
28	4.980087948353168E-4	0.0	0.0	23.53838568789125	0.0
29	4.980087948353168E-4	0.0	0.0	24.105949711071897	0.0
30	4.980087948353168E-4	0.0	0.0	24.738918889307584	0.0
31	4.980087948353168E-4	0.0	0.0	25.301004815745046	0.0
32	4.980087948353168E-4	0.0	0.0	25.880521050001743	0.0
33	4.980087948353168E-4	0.0	0.0	26.431318777089604	0.0
34	4.980087948353168E-4	0.0	0.0	26.96369017876856	0.0
35	4.980087948353168E-4	0.0	0.0	27.505689750480993	0.0
36	4.980087948353168E-4	0.0	0.0	28.04486727235603	0.0
37	4.980087948353168E-4	0.0	0.0	28.599815072734184	0.0
38	4.980087948353168E-4	0.0	0.0	29.143142667899514	0.0
39	4.980087948353168E-4	0.0	0.0	29.684644230817117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	20	7.0306094E-4	45.0	1
ACGGCGC	20	7.0306094E-4	45.0	8
CGCGGAT	25	3.8885883E-5	44.999996	4
GTATGCG	25	3.8885883E-5	44.999996	1
TCGTCGG	25	3.8885883E-5	44.999996	2
CGTTTTT	1860	0.0	42.94355	1
CGAAAGG	105	0.0	42.857143	2
CGGGACG	70	0.0	41.785717	6
ATTCGGA	65	0.0	41.53846	10
GATACGT	40	3.45457E-7	39.375	9
GCGCGAC	40	3.45457E-7	39.375	9
CATACGA	80	0.0	39.375	18
GCGCTAG	40	3.45457E-7	39.375	1
CGAACGG	35	6.2433373E-6	38.57143	2
GCGAACG	35	6.2433373E-6	38.57143	1
GTTAGCG	35	6.2433373E-6	38.57143	1
AATGCGG	35	6.2433373E-6	38.57143	2
TACGCGG	35	6.2433373E-6	38.57143	2
TTAAGCG	35	6.2433373E-6	38.57143	1
GCTAGCG	35	6.2433373E-6	38.57143	1
>>END_MODULE