SRR3550845_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528775
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	7581	1.4336910784360077	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	7193	1.3603139331473688	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	6049	1.1439648243581864	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2952	0.5582714765259326	Illumina PCR Primer Index 11 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1705	0.32244338329157013	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1623	0.3069358422769609	RNA PCR Primer, Index 41 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGACATCT	1466	0.2772445747245993	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	1398	0.2643846626637038	RNA PCR Primer, Index 41 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1358	0.25682000851023595	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTC	1138	0.21521441066616234	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1132	0.21407971254314215	RNA PCR Primer, Index 41 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTA	1104	0.20878445463571463	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	540	0.10212283107181694	RNA PCR Primer, Index 41 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGC	40	6.8030204E-9	45.0	4
CGACGGT	25	3.8879392E-5	45.0	28
ACGTTAG	20	7.029829E-4	45.0	1
TTTCGCG	20	7.029829E-4	45.0	1
ACGGGTA	25	3.8879392E-5	45.0	5
ATACCGA	20	7.029829E-4	45.0	41
TACGTAG	40	6.8030204E-9	45.0	1
TACCGAC	20	7.029829E-4	45.0	42
CACAACG	130	0.0	41.538464	12
TACGGCT	940	0.0	40.93085	7
CGTAAGC	50	1.0786607E-9	40.5	27
ATACGTA	50	1.0786607E-9	40.5	24
ACGTAGG	95	0.0	40.26316	2
AACGAGC	135	0.0	40.0	15
CGTTTTT	965	0.0	39.870464	1
ACGACCA	175	0.0	39.857143	28
TGCGGGC	130	0.0	39.807693	4
GCGAGAC	170	0.0	39.705883	21
CTACGGG	85	0.0	39.705883	3
CGATGAA	375	0.0	39.6	19