##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528775
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389874710415583	31.0	31.0	33.0	28.0	34.0
2	31.303204576615762	31.0	31.0	34.0	28.0	34.0
3	31.240054843742612	31.0	31.0	34.0	28.0	34.0
4	35.10048130112052	35.0	35.0	37.0	32.0	37.0
5	35.35017162309111	35.0	35.0	37.0	33.0	37.0
6	35.27712921374876	37.0	35.0	37.0	33.0	37.0
7	35.65092146943407	37.0	35.0	37.0	35.0	37.0
8	35.32647723511891	37.0	35.0	37.0	33.0	37.0
9	37.232096827573166	39.0	37.0	39.0	34.0	39.0
10	36.61724930263345	38.0	35.0	39.0	32.0	39.0
11	36.77197106519786	39.0	37.0	39.0	33.0	39.0
12	36.9273641908184	39.0	37.0	39.0	33.0	39.0
13	36.962475533071725	39.0	37.0	39.0	33.0	39.0
14	37.98245567585457	40.0	37.0	41.0	33.0	41.0
15	38.04156399224623	40.0	37.0	41.0	33.0	41.0
16	38.13599357004397	40.0	37.0	41.0	34.0	41.0
17	37.97064725072101	40.0	37.0	41.0	33.0	41.0
18	37.95612122358281	40.0	37.0	41.0	33.0	41.0
19	37.833823459883696	39.0	37.0	41.0	33.0	41.0
20	37.852701054323674	40.0	37.0	41.0	33.0	41.0
21	37.85017635099995	39.0	37.0	41.0	33.0	41.0
22	38.01183868375018	40.0	37.0	41.0	34.0	41.0
23	38.064008321119566	40.0	37.0	41.0	34.0	41.0
24	37.994364332655664	40.0	37.0	41.0	33.0	41.0
25	37.733540730934706	39.0	36.0	41.0	33.0	41.0
26	37.78548910217011	40.0	36.0	41.0	33.0	41.0
27	37.863293461302064	40.0	37.0	41.0	33.0	41.0
28	37.76950498794383	40.0	36.0	41.0	33.0	41.0
29	37.796034230060044	40.0	36.0	41.0	33.0	41.0
30	37.55157864876365	40.0	36.0	41.0	33.0	41.0
31	37.47851732778592	40.0	36.0	41.0	33.0	41.0
32	37.40233369580635	40.0	36.0	41.0	33.0	41.0
33	37.42636281972484	40.0	36.0	41.0	33.0	41.0
34	37.33962082171056	40.0	36.0	41.0	32.0	41.0
35	37.352469386790226	40.0	36.0	41.0	33.0	41.0
36	37.27338092761572	40.0	36.0	41.0	32.0	41.0
37	37.280147510755995	40.0	36.0	41.0	33.0	41.0
38	37.11248262493499	40.0	36.0	41.0	32.0	41.0
39	37.07590184861236	40.0	35.0	41.0	32.0	41.0
40	37.010405181788094	39.0	35.0	41.0	31.0	41.0
41	36.79527587348116	39.0	35.0	41.0	31.0	41.0
42	36.89348588719209	39.0	35.0	41.0	31.0	41.0
43	36.88947472932722	39.0	35.0	41.0	31.0	41.0
44	36.83509243061794	39.0	35.0	41.0	31.0	41.0
45	36.80292562999385	39.0	35.0	41.0	31.0	41.0
46	36.67386695664508	39.0	35.0	41.0	31.0	41.0
47	36.58594487258286	39.0	35.0	41.0	31.0	41.0
48	36.5031818826533	39.0	35.0	41.0	31.0	41.0
49	36.540957874332186	39.0	35.0	41.0	31.0	41.0
50	36.41626211526642	39.0	35.0	41.0	31.0	41.0
51	35.173831970119615	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	12.0
10	20.0
11	13.0
12	13.0
13	6.0
14	13.0
15	20.0
16	41.0
17	64.0
18	131.0
19	260.0
20	578.0
21	1094.0
22	1615.0
23	1997.0
24	2467.0
25	3098.0
26	3913.0
27	4492.0
28	5014.0
29	6012.0
30	7612.0
31	9854.0
32	13497.0
33	19417.0
34	32871.0
35	37104.0
36	41797.0
37	64347.0
38	119982.0
39	151380.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39624604037634	19.49260082265614	21.79622712874096	13.31492600822656
2	30.576521204671174	28.98851118150442	23.4640442532268	16.97092336059761
3	30.43941184813957	28.537090444896222	25.619592454257482	15.403905252706728
4	28.07697035601154	23.21932769136211	30.405370904448965	18.298331048177392
5	28.452366318377383	27.77778828424188	23.799725781286938	19.9701196160938
6	24.331331851921895	37.786582194695285	23.781381494964776	14.100704458418042
7	77.01025956219564	5.387924920807527	13.262351661859958	4.339463855136873
8	76.66833719445889	8.850267126849793	9.121270861897782	5.360124816793532
9	71.44097205805872	6.605834239515862	10.706822372464659	11.246371329960759
10	38.70058153278805	29.48380691220273	17.840102122831073	13.975509432178148
11	28.649614675429056	24.27497517847856	27.42395158621342	19.651458559878964
12	25.53222069878493	21.17422344097206	30.827100373504802	22.466455486738216
13	24.11744125573259	23.05782232518557	34.135312751170154	18.689423667911683
14	20.489244007375536	28.810741808897923	29.139993380927613	21.56002080279892
15	17.95470663325611	26.48574535482956	35.528911162592784	20.030636849321546
16	20.990591461396622	26.16897546215309	29.52011725213938	23.320315824310907
17	20.04255117961326	26.43808803366271	29.991206089546594	23.52815469717744
18	20.55902794194128	25.568909271429245	30.52545978913527	23.34660299749421
19	21.546215308968844	27.219705924069782	27.73883031535152	23.495248451609854
20	24.00226939624604	26.60205191243913	30.804217294690556	18.591461396624272
21	23.41695428112146	28.254739728618034	28.85064535955747	19.47766063070304
22	21.189730981986667	23.88879958394402	29.66611507730131	25.255354356768002
23	20.615006382676942	27.749420831166375	29.921611271334687	21.713961514821996
24	22.533780908704077	25.140560729989126	28.812254739728616	23.513403621578178
25	20.939530045860717	30.233842371519078	26.34428632215971	22.482341260460498
26	19.927568436480545	26.62436764219186	29.328542385702804	24.119521535624795
27	22.65349156068271	26.663325611082218	27.451184341165902	23.23199848706917
28	19.185475864025342	26.943028698406696	30.515247506028082	23.35624793153988
29	20.443666966100892	25.29601437284289	30.14382298709281	24.116495673963406
30	21.663656564701434	26.083683986572737	28.04935936835138	24.20330008037445
31	23.593967188312607	26.285660252470333	25.647014325563806	24.473358233653254
32	22.621152664176634	29.25724552030637	26.345988369344237	21.77561344617276
33	21.038627015271146	25.68105526925441	24.815847950451513	28.464469765022933
34	21.857689943737885	26.91106803460829	28.728476194978963	22.50276582667486
35	19.242210770176353	24.92080752683088	28.935558602430145	26.90142310056262
36	21.25951491655241	25.856744361968705	28.229398137203916	24.654342584274975
37	20.726963264148267	27.67282870786251	28.205191243912818	23.395016784076404
38	21.432178147605313	26.55382724221077	25.793012150725737	26.22098245945818
39	21.60427402959671	27.41865632830599	26.887617606732544	24.089452035364758
40	20.52914755803508	24.39071438702662	30.55023403148787	24.529904023450428
41	18.69698832206515	26.97177438418987	28.074700959765497	26.25653633397948
42	20.398846390241594	24.9162687343388	30.581816462578605	24.103068412841
43	22.154224386553828	24.01834428632216	27.274171433974754	26.55325989314926
44	21.406836556191198	24.234882511465177	28.60176823790837	25.75651269443525
45	21.059808046900855	23.043449482293983	28.011157864876363	27.8855846059288
46	23.845491938915416	25.301687863457996	27.22783792728476	23.624982270341828
47	18.91447212897735	24.83154460781996	32.71334688667202	23.54063637653066
48	20.92496808661529	25.267268687059712	27.762848092288785	26.044915134036216
49	20.167367973145478	23.163160134272612	32.328873339322016	24.34059855325989
50	21.083447591130444	22.43354924117063	30.037539596236584	26.44546357146234
51	19.28457283343577	23.090350338045482	27.458370762611693	30.16670606590705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	360.0
2	554.0
3	1619.0
4	2684.0
5	1905.0
6	1126.0
7	1089.5
8	1053.0
9	1059.5
10	1066.0
11	1064.0
12	1062.0
13	1080.0
14	1098.0
15	1084.0
16	1070.0
17	1010.5
18	951.0
19	1034.0
20	1117.0
21	1407.0
22	1697.0
23	1906.5
24	2116.0
25	2462.5
26	3665.0
27	4521.0
28	5404.5
29	6288.0
30	7558.5
31	8829.0
32	9779.5
33	10730.0
34	12459.0
35	14188.0
36	14965.0
37	15742.0
38	17518.0
39	19294.0
40	21043.5
41	22793.0
42	25300.0
43	27807.0
44	30650.0
45	33493.0
46	51709.5
47	69926.0
48	62159.5
49	54393.0
50	53099.5
51	51806.0
52	44280.0
53	36754.0
54	33039.0
55	29324.0
56	26471.5
57	23619.0
58	22046.5
59	20474.0
60	19293.0
61	18112.0
62	16275.5
63	14439.0
64	12341.0
65	10243.0
66	8282.0
67	6321.0
68	5209.5
69	4098.0
70	3268.0
71	2438.0
72	2303.0
73	2168.0
74	1663.5
75	883.0
76	607.0
77	489.5
78	372.0
79	255.5
80	139.0
81	117.0
82	95.0
83	57.5
84	20.0
85	14.0
86	8.0
87	5.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	528775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.14197952956902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.46649893136593	23.29216725231499
2	10.167241535279379	6.535905385982674
3	3.601761244672171	3.473032085899439
4	1.9438983078302219	2.4992295847137145
5	1.2073871336197428	1.9403906266535393
6	0.922836054468543	1.779706654312569
7	0.7913330252407005	1.7804506928850963
8	0.6634765687281288	1.7060360232326572
9	0.6274859686258372	1.8151777042797104
>10	7.498859915692711	45.25090638703544
>50	0.07202702900621404	1.564773999422379
>100	0.0295192741828746	1.6752152397405722
>500	5.90385483657492E-4	0.10247125390347858
>1k	0.005313469352917427	2.6331317021567946
>5k	0.001771156450972476	3.951405407466916
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	7581	1.4336910784360077	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	7193	1.3603139331473688	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	6049	1.1439648243581864	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2952	0.5582714765259326	Illumina PCR Primer Index 11 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1705	0.32244338329157013	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1623	0.3069358422769609	RNA PCR Primer, Index 41 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGACATCT	1466	0.2772445747245993	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	1398	0.2643846626637038	RNA PCR Primer, Index 41 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1358	0.25682000851023595	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTC	1138	0.21521441066616234	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1132	0.21407971254314215	RNA PCR Primer, Index 41 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTA	1104	0.20878445463571463	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	540	0.10212283107181694	RNA PCR Primer, Index 41 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8911635383669803E-4	0.0	0.0	0.22410287929648717	0.0
2	1.8911635383669803E-4	0.0	0.0	1.292042929412321	0.0
3	1.8911635383669803E-4	0.0	0.0	1.626400642995603	0.0
4	1.8911635383669803E-4	0.0	0.0	2.3739775897120703	0.0
5	1.8911635383669803E-4	0.0	0.0	4.941421209399083	0.0
6	1.8911635383669803E-4	0.0	0.0	5.833104817739114	0.0
7	1.8911635383669803E-4	0.0	0.0	6.802137014798355	0.0
8	1.8911635383669803E-4	0.0	0.0	8.112524230532836	0.0
9	1.8911635383669803E-4	0.0	0.0	8.52687816178904	0.0
10	1.8911635383669803E-4	0.0	0.0	10.563472176256441	0.0
11	1.8911635383669803E-4	0.0	0.0	11.945345373741194	0.0
12	1.8911635383669803E-4	0.0	0.0	14.496146754290578	0.0
13	1.8911635383669803E-4	0.0	0.0	15.041936551463287	0.0
14	1.8911635383669803E-4	0.0	0.0	15.27719729563614	0.0
15	1.8911635383669803E-4	0.0	0.0	15.84246607725403	0.0
16	1.8911635383669803E-4	0.0	0.0	16.55827147652593	0.0
17	1.8911635383669803E-4	0.0	0.0	17.398326320268545	0.0
18	1.8911635383669803E-4	0.0	0.0	18.280554110916743	0.0
19	1.8911635383669803E-4	0.0	0.0	19.119474256536336	0.0
20	1.8911635383669803E-4	0.0	0.0	19.720864261737034	0.0
21	1.8911635383669803E-4	0.0	0.0	20.36026665405891	0.0
22	1.8911635383669803E-4	0.0	0.0	21.12486407262068	0.0
23	1.8911635383669803E-4	0.0	0.0	21.817408160370668	0.0
24	1.8911635383669803E-4	0.0	0.0	22.360361212235826	0.0
25	1.8911635383669803E-4	0.0	0.0	22.81499692685925	0.0
26	1.8911635383669803E-4	0.0	0.0	23.24958630797598	0.0
27	1.8911635383669803E-4	0.0	0.0	23.727672450475154	0.0
28	1.8911635383669803E-4	0.0	0.0	24.204813011205143	0.0
29	1.8911635383669803E-4	0.0	0.0	24.722613588010024	0.0
30	1.8911635383669803E-4	0.0	0.0	25.26064961467543	0.0
31	1.8911635383669803E-4	0.0	0.0	25.77542432981892	0.0
32	1.8911635383669803E-4	0.0	0.0	26.265046569902132	0.0
33	1.8911635383669803E-4	0.0	0.0	26.73594629095551	0.0
34	1.8911635383669803E-4	0.0	0.0	27.218571225946764	0.0
35	3.7823270767339606E-4	0.0	0.0	27.733535057444094	0.0
36	3.7823270767339606E-4	0.0	0.0	28.23544986052669	0.0
37	3.7823270767339606E-4	0.0	0.0	28.72620679873292	0.0
38	5.673490615100941E-4	0.0	0.0	29.196539170724787	0.0
39	5.673490615100941E-4	0.0	0.0	29.713205049406646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGC	40	6.8030204E-9	45.0	4
CGACGGT	25	3.8879392E-5	45.0	28
ACGTTAG	20	7.029829E-4	45.0	1
TTTCGCG	20	7.029829E-4	45.0	1
ACGGGTA	25	3.8879392E-5	45.0	5
ATACCGA	20	7.029829E-4	45.0	41
TACGTAG	40	6.8030204E-9	45.0	1
TACCGAC	20	7.029829E-4	45.0	42
CACAACG	130	0.0	41.538464	12
TACGGCT	940	0.0	40.93085	7
CGTAAGC	50	1.0786607E-9	40.5	27
ATACGTA	50	1.0786607E-9	40.5	24
ACGTAGG	95	0.0	40.26316	2
AACGAGC	135	0.0	40.0	15
CGTTTTT	965	0.0	39.870464	1
ACGACCA	175	0.0	39.857143	28
TGCGGGC	130	0.0	39.807693	4
GCGAGAC	170	0.0	39.705883	21
CTACGGG	85	0.0	39.705883	3
CGATGAA	375	0.0	39.6	19
>>END_MODULE