FastQCFastQC Report
Sat 18 Jun 2016
SRR3550843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597568
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC81521.364196208632323No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC76141.2741646139016813No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG69561.1640516225768447No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC29190.4884799721537967No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTTCCGT16260.2721029238513441No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.24398896861947095No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT14160.23696047981150264No Hit
GAACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT13560.22691978151440506No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTA11430.19127530255970868No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC11370.19027123272999893No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC11040.18474884866659527No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT7940.1328719074649245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA207.030566E-445.09
TACACGA207.030566E-445.010
CGACAAT302.163746E-644.99999621
CGGTCTA302.163746E-644.99999631
ACAACGA1000.042.7513
CCGATGA5100.042.3529418
CCCGTCG650.041.53846440
TACGGCT9250.040.621627
GCGTATG501.0804797E-940.51
CGACCAC11300.040.22123712
CGATGAA5450.039.6330319
TGATACC8950.038.966484
CGACAGG1100.038.8636362
CACAACG1100.038.86363612
CGTTCAT356.2432555E-638.57142617
GCGTAAG356.2432555E-638.5714261
TCAACGG356.2432555E-638.5714262
GATACCT9000.038.55
ACGGCTG9800.038.3418358
CCACCGA7350.038.26530515