##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597568
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.335822868694443	31.0	31.0	33.0	28.0	34.0
2	31.24645228660169	31.0	31.0	34.0	28.0	34.0
3	31.202144358466317	31.0	31.0	34.0	28.0	34.0
4	35.06516580539788	35.0	35.0	37.0	32.0	37.0
5	35.32406688443826	35.0	35.0	37.0	33.0	37.0
6	35.2422753561101	37.0	35.0	37.0	33.0	37.0
7	35.60969295544607	37.0	35.0	37.0	35.0	37.0
8	35.23173764324729	37.0	35.0	37.0	32.0	37.0
9	37.12507697868694	39.0	37.0	39.0	34.0	39.0
10	36.54061127771233	38.0	35.0	39.0	32.0	39.0
11	36.700106096712005	39.0	35.0	39.0	32.0	39.0
12	36.82479483506479	39.0	35.0	39.0	33.0	39.0
13	36.85089228338867	39.0	37.0	39.0	33.0	39.0
14	37.85554280014994	40.0	37.0	41.0	33.0	41.0
15	37.94391098586269	40.0	37.0	41.0	33.0	41.0
16	38.02636687372818	40.0	37.0	41.0	33.0	41.0
17	37.83680518367784	40.0	36.0	41.0	33.0	41.0
18	37.82151822052051	39.0	37.0	41.0	33.0	41.0
19	37.70686516011567	39.0	36.0	41.0	33.0	41.0
20	37.71206456838385	39.0	36.0	41.0	33.0	41.0
21	37.70469302238406	39.0	36.0	41.0	33.0	41.0
22	37.84136366070472	40.0	36.0	41.0	33.0	41.0
23	37.89327072400128	40.0	36.0	41.0	33.0	41.0
24	37.80185016600621	40.0	36.0	41.0	33.0	41.0
25	37.54997924922352	39.0	36.0	41.0	33.0	41.0
26	37.59147243493628	39.0	36.0	41.0	33.0	41.0
27	37.64052459301703	40.0	36.0	41.0	33.0	41.0
28	37.54979014940559	40.0	36.0	41.0	33.0	41.0
29	37.5833679179608	40.0	36.0	41.0	33.0	41.0
30	37.342762664667454	39.0	36.0	41.0	32.0	41.0
31	37.36021005140837	39.0	36.0	41.0	32.0	41.0
32	37.27049808557352	39.0	36.0	41.0	32.0	41.0
33	37.25685779693692	40.0	36.0	41.0	32.0	41.0
34	37.22743353057727	40.0	36.0	41.0	32.0	41.0
35	37.13628072453679	40.0	35.0	41.0	32.0	41.0
36	37.04196509853272	40.0	35.0	41.0	32.0	41.0
37	37.018018033094144	40.0	35.0	41.0	32.0	41.0
38	36.92738901681482	39.0	35.0	41.0	31.0	41.0
39	36.847687627182175	39.0	35.0	41.0	31.0	41.0
40	36.72272946342508	39.0	35.0	41.0	31.0	41.0
41	36.59786333940238	39.0	35.0	41.0	31.0	41.0
42	36.64597501874264	39.0	35.0	41.0	31.0	41.0
43	36.58072386740923	39.0	35.0	41.0	31.0	41.0
44	36.514709623005245	39.0	35.0	41.0	31.0	41.0
45	36.527898080218485	39.0	35.0	41.0	31.0	41.0
46	36.380003614651386	39.0	35.0	40.0	31.0	41.0
47	36.29684487790511	39.0	35.0	40.0	31.0	41.0
48	36.21227207614866	39.0	35.0	40.0	30.0	41.0
49	36.24904111331263	39.0	35.0	40.0	30.0	41.0
50	36.10106966905858	38.0	35.0	40.0	30.0	41.0
51	34.91288690157438	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	19.0
10	25.0
11	22.0
12	13.0
13	19.0
14	17.0
15	32.0
16	59.0
17	112.0
18	177.0
19	353.0
20	771.0
21	1305.0
22	2052.0
23	2595.0
24	3210.0
25	3803.0
26	4724.0
27	5385.0
28	6124.0
29	7185.0
30	9249.0
31	12017.0
32	16188.0
33	23091.0
34	40793.0
35	44492.0
36	48442.0
37	74226.0
38	131860.0
39	159179.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.729744564635325	19.076490039627288	21.58984416836243	12.60392122737496
2	30.55602040269894	29.19450171361251	23.34813778515583	16.901340098532717
3	32.07835760951055	28.037143889900396	24.497965085145122	15.386533415443932
4	27.91715754525008	23.89100487308557	29.60533361893542	18.58650396272893
5	28.602602548998608	28.802579790082465	23.312493306201134	19.28232435471779
6	25.365146728071114	38.09056040484096	22.489825425725606	14.054467441362323
7	76.49740280604048	5.7904707079361675	13.479135428938632	4.232991057084717
8	76.25508728713719	9.778468726571704	8.703277283924173	5.263166702366927
9	70.38981337688766	7.4389860233479705	10.30326255756667	11.867938042197707
10	41.84394077326765	24.87147906179715	17.989082414051623	15.295497750883582
11	31.867837635214734	24.173985220092106	24.11407572025276	19.8441014244404
12	27.418302184855946	21.118098693370463	28.50035477133983	22.963244350433758
13	25.98700064260469	22.489490735782372	31.472401467280708	20.051107154332225
14	21.278415176180786	28.549888882938845	27.385837260362	22.785858680518366
15	19.721772250187428	25.70017136125094	33.78427225018743	20.79378413837421
16	22.522625040162794	25.18240601906394	27.842521687908324	24.452447252864946
17	21.67602682874585	26.27784620327728	28.881399271714685	23.164727696262183
18	22.306582681803576	25.764097140409127	28.352254471457645	23.577065706329655
19	23.291407839777232	26.96211979222448	26.064816054407196	23.68165631359109
20	25.701677465995505	26.672780336296455	28.65749169968941	18.968050498018634
21	24.253474081610797	28.49633849202099	27.324589000749704	19.925598425618507
22	22.37670022491164	23.905061850701507	28.613647317125412	25.104590607261436
23	23.015623326550283	27.61242235193317	27.676515476062974	21.69543884545357
24	23.35784379350969	25.554581235943026	26.73821222019921	24.349362750348078
25	21.879350969262077	30.128119310270968	25.05589322052051	22.93663649994645
26	21.230554514297957	26.15551702902431	27.510007229302776	25.103921227374958
27	23.94053898468459	27.4507671093499	26.15166809467709	22.45702581128842
28	19.751559655135484	27.275556924065548	28.581349737603084	24.391533683195888
29	22.183751472635752	25.22842588625897	27.28509558744779	25.30272705365749
30	21.66347595587448	28.12031434079469	27.32107475634572	22.895134946985113
31	23.216102602549	25.513916407839776	23.941543054514298	27.32843793509693
32	23.383280229195673	27.237402270536577	26.11669299560887	23.262624504658884
33	22.6178443290136	25.46689247081504	26.534218699796508	25.381044500374855
34	21.56039145335761	24.427178162150582	26.82021125629217	27.192219128199635
35	21.211477187533472	25.287666006211847	26.06381198457749	27.437044821677198
36	22.682104798115027	26.599483238727643	24.2846002463318	26.433811716825534
37	21.096511192031702	27.880341651494057	27.721531273428297	23.301615883045944
38	21.00832039198886	25.432754096604903	26.230487576309308	27.32843793509693
39	23.25844088036843	23.181294848452396	28.381874531434082	25.1783897397451
40	21.659961711470494	23.191168201777874	29.939186837313912	25.20968324943772
41	20.725340044982328	25.49350032130235	25.74167291421227	28.039486719503053
42	20.92314180143515	24.52089134625683	28.31794875227589	26.23801810003213
43	24.302673503266572	23.491050390917852	26.547271607582733	25.659004498232836
44	22.114972689300632	24.345346471029238	28.010870729356323	25.528810110313806
45	21.247456356431403	22.91521634357931	26.970487040805395	28.86684025918389
46	24.36492583270858	25.196797686623114	26.06130181000321	24.376974670665096
47	20.646353218378493	24.257490360929634	31.259203973439003	23.836952447252866
48	21.391373032023132	24.583645710613688	27.353372335868052	26.671608921495128
49	20.943390543000966	23.48385455713827	31.17553148762986	24.397223412230908
50	21.66113312627182	22.895971671843203	29.47965754525008	25.96323765663489
51	20.82524499303845	22.505890543000966	27.056837046160435	29.612027417800153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	191.0
1	486.5
2	782.0
3	1862.5
4	2943.0
5	2093.0
6	1243.0
7	1170.0
8	1097.0
9	1066.5
10	1036.0
11	1013.5
12	991.0
13	1048.0
14	1105.0
15	1082.5
16	1060.0
17	1055.5
18	1051.0
19	1039.0
20	1027.0
21	1253.5
22	1480.0
23	1770.0
24	2060.0
25	2634.5
26	3816.5
27	4424.0
28	5212.0
29	6000.0
30	7109.5
31	8219.0
32	9759.0
33	11299.0
34	12416.5
35	13534.0
36	14874.0
37	16214.0
38	17314.5
39	18415.0
40	21466.5
41	24518.0
42	25995.0
43	27472.0
44	30134.5
45	32797.0
46	35598.5
47	38400.0
48	57217.5
49	76035.0
50	65995.5
51	55956.0
52	54929.0
53	53902.0
54	46646.0
55	39390.0
56	36684.5
57	33979.0
58	30417.0
59	26855.0
60	26449.0
61	26043.0
62	23381.0
63	20719.0
64	17623.5
65	14528.0
66	12297.5
67	10067.0
68	8685.0
69	7303.0
70	5887.0
71	4471.0
72	3920.0
73	3369.0
74	2722.0
75	1588.5
76	1102.0
77	862.0
78	622.0
79	483.0
80	344.0
81	244.0
82	144.0
83	90.5
84	37.0
85	31.5
86	26.0
87	16.5
88	7.0
89	9.5
90	12.0
91	11.0
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.42855091918418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65189235743236	22.10691805973218
2	9.748452052292949	5.932625393128429
3	3.4539245090812125	3.1529375338678762
4	1.7526249263890035	2.1331934725943684
5	1.1874643806900214	1.8066410186271913
6	0.9425534386069421	1.7208321180422081
7	0.7444758369537513	1.5857324638994637
8	0.697400820467046	1.6976717101329863
9	0.6210853852623808	1.7008855443555724
>10	8.061589258812504	47.925325388511666
>50	0.08996628105157872	1.845406667551878
>100	0.04194746846576608	2.4003050684975524
>500	5.519403745495537E-4	0.13335028158319734
>1k	0.00441552299639643	2.0420731407683834
>5k	0.0016558211236486608	3.8161021387070657
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	8152	1.364196208632323	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	7614	1.2741646139016813	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG	6956	1.1640516225768447	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2919	0.4884799721537967	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTTCCGT	1626	0.2721029238513441	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1458	0.24398896861947095	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	1416	0.23696047981150264	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	1356	0.22691978151440506	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTA	1143	0.19127530255970868	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1137	0.19027123272999893	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	1104	0.18474884866659527	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	794	0.1328719074649245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.346899432365856E-4	0.0	0.0	0.1395657063296562	0.0
2	3.346899432365856E-4	0.0	0.0	0.9936944414694228	0.0
3	3.346899432365856E-4	0.0	0.0	1.240695619578023	0.0
4	3.346899432365856E-4	0.0	0.0	1.7845667773374745	0.0
5	3.346899432365856E-4	0.0	0.0	4.013434454321517	0.0
6	3.346899432365856E-4	0.0	0.0	4.779037699475206	0.0
7	3.346899432365856E-4	0.0	0.0	5.615093177680197	0.0
8	3.346899432365856E-4	0.0	0.0	6.7132108814394345	0.0
9	3.346899432365856E-4	0.0	0.0	7.076014779907894	0.0
10	3.346899432365856E-4	0.0	0.0	9.00064929848988	0.0
11	3.346899432365856E-4	0.0	0.0	10.054587929741887	0.0
12	3.346899432365856E-4	0.0	0.0	12.367295437506694	0.0
13	5.020349148548784E-4	0.0	0.0	12.797372014565706	0.0
14	5.020349148548784E-4	0.0	0.0	13.001030845025168	0.0
15	5.020349148548784E-4	0.0	0.0	13.491853646781621	0.0
16	5.020349148548784E-4	0.0	0.0	14.063336724858091	0.0
17	5.020349148548784E-4	0.0	0.0	14.71631680411267	0.0
18	5.020349148548784E-4	0.0	0.0	15.387035450358788	0.0
19	5.020349148548784E-4	0.0	0.0	16.111639177465996	0.0
20	5.020349148548784E-4	0.0	0.0	16.56313591089215	0.0
21	5.020349148548784E-4	0.0	0.0	17.066007550605118	0.0
22	5.020349148548784E-4	0.0	0.0	17.64836805183678	0.0
23	5.020349148548784E-4	0.0	0.0	18.173831262718217	0.0
24	5.020349148548784E-4	0.0	0.0	18.635034004498234	0.0
25	5.020349148548784E-4	0.0	0.0	19.04201697547392	0.0
26	5.020349148548784E-4	0.0	0.0	19.430591999571597	0.0
27	5.020349148548784E-4	0.0	0.0	19.834730106029774	0.0
28	5.020349148548784E-4	0.0	0.0	20.213766466745206	0.0
29	5.020349148548784E-4	0.0	0.0	20.62242288743708	0.0
30	8.36724858091464E-4	0.0	0.0	21.058356538502732	0.0
31	8.36724858091464E-4	0.0	0.0	21.448102977401735	0.0
32	8.36724858091464E-4	0.0	0.0	21.876171414801327	0.0
33	8.36724858091464E-4	0.0	0.0	22.283154385777017	0.0
34	8.36724858091464E-4	0.0	0.0	22.67792117382457	0.0
35	8.36724858091464E-4	0.0	0.0	23.09143059869337	0.0
36	8.36724858091464E-4	0.0	0.0	23.494731980293455	0.0
37	8.36724858091464E-4	0.0	0.0	23.907404680304165	0.0
38	8.36724858091464E-4	0.0	0.0	24.298489878976117	0.0
39	8.36724858091464E-4	0.0	0.0	24.754672271607582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	20	7.030566E-4	45.0	9
TACACGA	20	7.030566E-4	45.0	10
CGACAAT	30	2.163746E-6	44.999996	21
CGGTCTA	30	2.163746E-6	44.999996	31
ACAACGA	100	0.0	42.75	13
CCGATGA	510	0.0	42.35294	18
CCCGTCG	65	0.0	41.538464	40
TACGGCT	925	0.0	40.62162	7
GCGTATG	50	1.0804797E-9	40.5	1
CGACCAC	1130	0.0	40.221237	12
CGATGAA	545	0.0	39.63303	19
TGATACC	895	0.0	38.96648	4
CGACAGG	110	0.0	38.863636	2
CACAACG	110	0.0	38.863636	12
CGTTCAT	35	6.2432555E-6	38.571426	17
GCGTAAG	35	6.2432555E-6	38.571426	1
TCAACGG	35	6.2432555E-6	38.571426	2
GATACCT	900	0.0	38.5	5
ACGGCTG	980	0.0	38.341835	8
CCACCGA	735	0.0	38.265305	15
>>END_MODULE