FastQCFastQC Report
Sat 18 Jun 2016
SRR3550840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596001
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC37060.6218110372298034No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC28430.47701262246204285No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG24360.40872414643599597No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC16150.27097269971023535No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.20335536349771224No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT8040.13489910251828435No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC7110.11929510185385594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGT253.888538E-545.09
ATACGCG302.163737E-644.9999961
AAACGGG700.041.7857133
CGATGAA1850.040.13513619
GCGTGAG451.9255822E-840.01
TACGGCT4000.038.81257
CGATCAA356.243228E-638.57142610
TAATGCG356.243228E-638.5714261
GTACGAG356.243228E-638.5714261
TTGCGAG659.094947E-1238.076921
ACGGCTG4100.037.8658528
CGTTTTT7850.037.8343961
TACGGGA2100.037.5000044
TGCGGGA5100.037.54
ATTAGCG301.1392626E-437.4999961
GTACTAG601.546141E-1037.4999961
TGCAACG301.1392626E-437.4999961
TTGCGGG2500.036.93
TGGGCGA2200.036.818186
AACGGGA2150.036.6279074
Produced by FastQC (version 0.11.5)