##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596001
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409146964518516	31.0	31.0	33.0	28.0	34.0
2	31.32967226565056	31.0	31.0	34.0	28.0	34.0
3	31.25975627557672	31.0	31.0	34.0	28.0	34.0
4	35.10913740077617	37.0	35.0	37.0	32.0	37.0
5	35.37508494113265	37.0	35.0	37.0	33.0	37.0
6	35.29818909699816	37.0	35.0	37.0	33.0	37.0
7	35.72320012885884	37.0	35.0	37.0	35.0	37.0
8	35.448161999728185	37.0	35.0	37.0	33.0	37.0
9	37.417105004857376	39.0	37.0	39.0	34.0	39.0
10	36.70795015444605	39.0	35.0	39.0	32.0	39.0
11	36.82987109082032	39.0	37.0	39.0	33.0	39.0
12	37.00163422544593	39.0	37.0	39.0	33.0	39.0
13	37.097333729305824	39.0	37.0	39.0	33.0	39.0
14	38.13700480368322	40.0	37.0	41.0	33.0	41.0
15	38.264964320529664	40.0	38.0	41.0	34.0	41.0
16	38.27011028504986	40.0	38.0	41.0	34.0	41.0
17	38.14861887815624	40.0	37.0	41.0	33.0	41.0
18	38.131285014622456	40.0	37.0	41.0	34.0	41.0
19	37.98905874319003	40.0	37.0	41.0	34.0	41.0
20	38.11027162706103	40.0	37.0	41.0	34.0	41.0
21	37.990802028855654	40.0	37.0	41.0	33.0	41.0
22	38.151622228821765	40.0	37.0	41.0	34.0	41.0
23	38.24024288549852	40.0	37.0	41.0	34.0	41.0
24	38.16365240997918	40.0	37.0	41.0	34.0	41.0
25	37.82474022694593	40.0	37.0	41.0	33.0	41.0
26	37.92606723814222	40.0	37.0	41.0	33.0	41.0
27	38.01871137800104	40.0	37.0	41.0	34.0	41.0
28	37.91042968048711	40.0	37.0	41.0	33.0	41.0
29	37.946934652794205	40.0	37.0	41.0	34.0	41.0
30	37.67450557968863	40.0	36.0	41.0	33.0	41.0
31	37.66322371942329	40.0	36.0	41.0	33.0	41.0
32	37.61864829085857	40.0	36.0	41.0	33.0	41.0
33	37.638866377740975	40.0	37.0	41.0	33.0	41.0
34	37.579822852646224	40.0	37.0	41.0	33.0	41.0
35	37.56290174009775	40.0	37.0	41.0	33.0	41.0
36	37.4496217288226	40.0	36.0	41.0	33.0	41.0
37	37.47014518432016	40.0	36.0	41.0	33.0	41.0
38	37.32030986525191	40.0	36.0	41.0	33.0	41.0
39	37.26615559369867	40.0	36.0	41.0	32.0	41.0
40	37.157630608002336	40.0	36.0	41.0	32.0	41.0
41	36.9895486752539	39.0	35.0	41.0	31.0	41.0
42	37.04149321897111	39.0	35.0	41.0	32.0	41.0
43	36.995302021305335	39.0	35.0	41.0	32.0	41.0
44	36.93199004699657	39.0	35.0	41.0	31.0	41.0
45	36.86513781017146	39.0	35.0	41.0	31.0	41.0
46	36.73496688763945	39.0	35.0	41.0	31.0	41.0
47	36.68127570255755	39.0	35.0	41.0	31.0	41.0
48	36.57122387378545	39.0	35.0	41.0	31.0	41.0
49	36.550140016543594	39.0	35.0	41.0	31.0	41.0
50	36.41717547453779	39.0	35.0	40.0	31.0	41.0
51	35.213502997478194	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	17.0
10	21.0
11	18.0
12	14.0
13	17.0
14	21.0
15	46.0
16	44.0
17	96.0
18	176.0
19	322.0
20	556.0
21	903.0
22	1260.0
23	1734.0
24	2287.0
25	3243.0
26	4027.0
27	4840.0
28	5693.0
29	6607.0
30	8177.0
31	10587.0
32	14389.0
33	20079.0
34	35497.0
35	40096.0
36	47050.0
37	74956.0
38	137851.0
39	175338.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17173628903307	19.82446338177285	22.775968496697153	12.227831832496925
2	31.85363782946673	25.774285613614744	25.08452167026565	17.287554886652874
3	33.13685715292424	25.367239316712553	25.606500660233795	15.889402870129413
4	29.214212727830997	25.295259571712126	26.34206989585588	19.148457804600998
5	26.953646050929446	30.195754705109557	23.75130243070062	19.09929681326038
6	26.597438594901686	37.133662527411865	22.33452628435187	13.934372593334574
7	81.0673136454469	5.591265786466802	9.229850285486098	4.111570282600197
8	82.4097610574479	6.280190805048985	7.302504525999118	4.0075436115040075
9	75.83208753005448	7.932537025944588	8.931360853421387	7.304014590579546
10	38.319566577908425	32.30615384873515	16.15064404254355	13.223635530812869
11	28.330992733233668	25.504655193531555	27.26287372001054	18.901478353224242
12	26.67881429729145	23.33972594005715	29.635688530723943	20.34577123192746
13	24.618918424633517	23.844087509920286	31.69105420964059	19.84593985580561
14	20.581005736567555	28.524616569435285	29.153139004800327	21.74123868919683
15	19.970436291214277	28.02746975256753	32.611858033795244	19.39023592242295
16	23.156840340871913	26.866397875171348	29.72880917985037	20.24795260410637
17	22.064056939501782	27.564215496282724	28.822770431593238	21.54895713262226
18	23.21673956922891	27.157169199380537	28.536193731218574	21.08989750017198
19	23.521269259615334	28.023442913686385	27.08518945438011	21.370098372318168
20	26.195090276694167	27.05549151763168	28.467737470239147	18.281680735435007
21	24.968246697572656	27.552470549546058	28.162368855085813	19.316913897795473
22	23.199289934077292	24.845931466557943	29.14693096152523	22.807847637839533
23	22.57580104731368	27.382672176724537	29.18199801678185	20.859528759179934
24	22.30029815386216	26.41774090983069	29.271762966840658	22.010197969466493
25	22.591237263024727	28.80230066728076	26.85515628329483	21.751305786399687
26	22.408855018699633	28.217066749888005	27.745255460980772	21.628822770431594
27	23.458182117144098	27.924953146051767	27.541564527576295	21.075300209227837
28	20.83050196224503	28.314885377709096	28.88183073518333	21.97278192486254
29	23.638718726982002	26.65482104895797	27.778812451656957	21.927647772403066
30	24.2843552275919	26.372774542324596	28.111362229258003	21.231508000825503
31	23.810866089150856	27.10985384252711	25.571265820023793	23.50801424829824
32	24.206503009223137	29.053642527445422	25.713547460490837	21.0263070028406
33	23.565396702354526	27.573275883765298	26.245929117568593	22.615398296311582
34	22.859022048620727	28.323274625378147	27.073612292596827	21.744091033404306
35	23.0627129820252	26.921766909787063	26.48166697706883	23.533853131118907
36	23.44643717040743	28.13720111207867	26.60985468145188	21.80650703606202
37	22.961370870183103	28.588039281813288	27.091733067561968	21.35885678044164
38	22.092077026716396	27.647101263252914	25.996097321984358	24.26472438804633
39	23.093920983354053	27.313712560884966	27.059015001652682	22.5333514541083
40	23.852980112449476	25.735695074337123	28.574113130682665	21.837211682530732
41	20.545267541497413	27.674450210654012	28.646931800449998	23.13335044739858
42	21.98352016187892	26.60851240182483	28.241898922988383	23.166068513307863
43	23.425296266281432	26.178982921169595	27.645926768579248	22.749794043969725
44	23.107511564577912	26.46723747107807	27.292571656758962	23.132679307585054
45	22.604324489388443	25.123112209543276	27.111531692060918	25.161031609007367
46	23.46690693471991	26.97545809486897	26.332338368559782	23.22529660185134
47	22.242076775038967	26.25549285991131	29.65162810129513	21.85080226375459
48	21.71590316123631	26.143915865912977	28.078979733255483	24.06120123959524
49	21.68150724579321	25.786701700164933	29.80263455933799	22.72915649470387
50	21.33704473650212	25.647104618951982	29.184514791082567	23.831335853463333
51	21.20499797819131	24.825629487198846	27.60012147630625	26.369251058303593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	461.0
2	776.0
3	1634.0
4	2492.0
5	1796.5
6	1101.0
7	1049.5
8	998.0
9	992.5
10	987.0
11	991.0
12	995.0
13	1076.0
14	1157.0
15	1147.5
16	1138.0
17	1108.0
18	1078.0
19	1248.0
20	1418.0
21	1668.5
22	1919.0
23	2326.5
24	2734.0
25	3267.5
26	4609.5
27	5418.0
28	6766.5
29	8115.0
30	9983.5
31	11852.0
32	12830.5
33	13809.0
34	16156.5
35	18504.0
36	20329.0
37	22154.0
38	23546.5
39	24939.0
40	27810.5
41	30682.0
42	31179.5
43	31677.0
44	34573.0
45	37469.0
46	39173.0
47	40877.0
48	49488.0
49	58099.0
50	54992.5
51	51886.0
52	51304.0
53	50722.0
54	44998.0
55	39274.0
56	35058.0
57	30842.0
58	28641.5
59	26441.0
60	24685.0
61	22929.0
62	20538.0
63	18147.0
64	15408.0
65	12669.0
66	10143.0
67	7617.0
68	5977.0
69	4337.0
70	3728.5
71	3120.0
72	2446.0
73	1772.0
74	1364.0
75	745.5
76	535.0
77	367.5
78	200.0
79	161.0
80	122.0
81	86.5
82	51.0
83	40.0
84	29.0
85	18.0
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	596001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.92643495956546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43866744326758	22.093433022822413
2	8.566531492187702	5.2986455804442425
3	3.193437893063672	2.962849478917362
4	1.9303878491754924	2.3880005705704526
5	1.4074564262977003	2.176380481315913
6	1.2249718721084542	2.2730407758035542
7	1.0401414495012962	2.2517506820724984
8	1.0142320591159282	2.5093265448123923
9	0.93280795134547	2.5963581993346057
>10	9.149352303471437	49.797702033083354
>50	0.06491752938699082	1.365888916521856
>100	0.03218600196497463	1.8546463512010039
>500	0.0021821018281338728	0.4391562314360241
>1k	0.0027276272851673414	1.9928211316643554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	3706	0.6218110372298034	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	2843	0.47701262246204285	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	2436	0.40872414643599597	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1615	0.27097269971023535	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.20335536349771224	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	804	0.13489910251828435	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	711	0.11929510185385594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.033548601428521E-4	0.0	0.0	0.10503338081647515	0.0
2	5.033548601428521E-4	0.0	0.0	0.6147640691878034	0.0
3	5.033548601428521E-4	0.0	0.0	0.8149315185712775	0.0
4	5.033548601428521E-4	0.0	0.0	1.1906020291912263	0.0
5	5.033548601428521E-4	0.0	0.0	2.5437876782085937	0.0
6	5.033548601428521E-4	0.0	0.0	3.2184509757533966	0.0
7	5.033548601428521E-4	0.0	0.0	3.801168118845438	0.0
8	5.033548601428521E-4	0.0	0.0	4.6243211001323825	0.0
9	5.033548601428521E-4	0.0	0.0	4.914421284528046	0.0
10	5.033548601428521E-4	0.0	0.0	5.932875951550417	0.0
11	5.033548601428521E-4	0.0	0.0	6.786062439492551	0.0
12	5.033548601428521E-4	0.0	0.0	7.974483264289825	0.0
13	5.033548601428521E-4	0.0	0.0	8.28756998729868	0.0
14	6.711398135238029E-4	0.0	0.0	8.43421403655363	0.0
15	6.711398135238029E-4	0.0	0.0	8.717267252907293	0.0
16	6.711398135238029E-4	0.0	0.0	9.167434282828385	0.0
17	6.711398135238029E-4	0.0	0.0	9.717936714871284	0.0
18	8.389247669047535E-4	0.0	0.0	10.273976050375754	0.0
19	8.389247669047535E-4	0.0	0.0	10.711894778700035	0.0
20	8.389247669047535E-4	0.0	0.0	11.073471353235984	0.0
21	8.389247669047535E-4	0.0	0.0	11.514913565581265	0.0
22	8.389247669047535E-4	0.0	0.0	12.045617373125213	0.0
23	8.389247669047535E-4	0.0	0.0	12.513401823151304	0.0
24	8.389247669047535E-4	0.0	0.0	12.907696463596537	0.0
25	8.389247669047535E-4	0.0	0.0	13.264910629344582	0.0
26	8.389247669047535E-4	0.0	0.0	13.571118169264818	0.0
27	8.389247669047535E-4	0.0	0.0	13.92816455005948	0.0
28	8.389247669047535E-4	0.0	0.0	14.278499532718905	0.0
29	8.389247669047535E-4	0.0	0.0	14.63252578435271	0.0
30	8.389247669047535E-4	0.0	0.0	15.054672727059183	0.0
31	8.389247669047535E-4	0.0	0.0	15.43017545272575	0.0
32	8.389247669047535E-4	0.0	0.0	15.788899683054224	0.0
33	8.389247669047535E-4	0.0	0.0	16.159536645072745	0.0
34	8.389247669047535E-4	0.0	0.0	16.5298380371845	0.0
35	8.389247669047535E-4	0.0	0.0	16.92312596790945	0.0
36	8.389247669047535E-4	0.0	0.0	17.318930672935114	0.0
37	0.0010067097202857043	0.0	0.0	17.704164925897775	0.0
38	0.0010067097202857043	0.0	0.0	18.090741458487486	0.0
39	0.0010067097202857043	0.0	0.0	18.49426427136867	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTCGT	25	3.888538E-5	45.0	9
ATACGCG	30	2.163737E-6	44.999996	1
AAACGGG	70	0.0	41.785713	3
CGATGAA	185	0.0	40.135136	19
GCGTGAG	45	1.9255822E-8	40.0	1
TACGGCT	400	0.0	38.8125	7
CGATCAA	35	6.243228E-6	38.571426	10
TAATGCG	35	6.243228E-6	38.571426	1
GTACGAG	35	6.243228E-6	38.571426	1
TTGCGAG	65	9.094947E-12	38.07692	1
ACGGCTG	410	0.0	37.865852	8
CGTTTTT	785	0.0	37.834396	1
TACGGGA	210	0.0	37.500004	4
TGCGGGA	510	0.0	37.5	4
ATTAGCG	30	1.1392626E-4	37.499996	1
GTACTAG	60	1.546141E-10	37.499996	1
TGCAACG	30	1.1392626E-4	37.499996	1
TTGCGGG	250	0.0	36.9	3
TGGGCGA	220	0.0	36.81818	6
AACGGGA	215	0.0	36.627907	4
>>END_MODULE