SRR3550839_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	601151
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2830	0.47076358518907896	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	2762	0.4594519513400127	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	2280	0.37927242905692576	No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2081	0.3461692652927467	TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1348	0.22423650630207717	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1312	0.21824799426433625	No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	837	0.13923290487747672	TruSeq Adapter, Index 16 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	640	0.10646243622650549	TruSeq Adapter, Index 16 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTTG	30	2.163768E-6	45.000004	29
TTTAGCG	20	7.0305984E-4	45.0	1
ACGTTAT	20	7.0305984E-4	45.0	6
GACCGAC	20	7.0305984E-4	45.0	9
TAAACGG	20	7.0305984E-4	45.0	2
TACGGGT	50	2.1827873E-11	45.0	4
TTACGAG	20	7.0305984E-4	45.0	1
ACCGTTG	20	7.0305984E-4	45.0	32
GACCGAT	150	0.0	43.500004	9
TGCACGG	130	0.0	43.26923	2
TACGTGC	50	1.0804797E-9	40.5	16
ACGTAAG	45	1.925764E-8	40.0	1
TAGCCGT	40	3.454552E-7	39.375	44
TGCGACG	40	3.454552E-7	39.375	1
GCGCTAG	40	3.454552E-7	39.375	1
ACATGCG	35	6.243319E-6	38.571426	31
CGGTCTA	35	6.243319E-6	38.571426	31
TCGACAC	35	6.243319E-6	38.571426	34
GTACGAG	35	6.243319E-6	38.571426	1
CGTTTTT	845	0.0	38.076923	1