FastQCFastQC Report
Sat 18 Jun 2016
SRR3550837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343760
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC34330.9986618571096114No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC33910.9864440307191062No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG31040.9029555503839888No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC15510.45118687456364903TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC10660.31010006981615085TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT7590.22079357691412613No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.21817547125901793No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT6950.2021759367000233No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT6880.20013963230160578No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT6310.18355829648592042TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA5100.1483593204561322No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC5090.14806841982778682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG351.2086093E-745.0000042
CAACCGG253.8850812E-545.0000042
CTACGCA253.8850812E-545.00000426
GCTACGC253.8850812E-545.00000425
TAATACG207.0263806E-445.037
TACGGGT207.0263806E-445.04
GCGCAAT207.0263806E-445.019
CGCAATC207.0263806E-445.020
CAACTCC207.0263806E-445.028
GACGACT207.0263806E-445.014
AATCGGA207.0263806E-445.014
ACGTTAG302.1610467E-644.9999961
GCCGATG302.1610467E-644.9999969
GGCTACG302.1610467E-644.9999961
CGTTTTT5200.041.5384641
CTGTGCG556.002665E-1140.9090921
TGCACGG403.448804E-739.3752
TACGGCT3950.039.3037957
TCGAGAG356.2355175E-638.571431
GGCGATA356.2355175E-638.571438
Produced by FastQC (version 0.11.5)