FastQCFastQC Report
Sat 18 Jun 2016
SRR3550836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472460
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC87681.8558184819878931No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC84961.798247470685349No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG72581.5362147060068576No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC31100.6582567836430597Illumina PCR Primer Index 5 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT17850.37780976167294583Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCATGATTT16860.3568556068238581No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT15410.32616517800448713No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.28171697074884644No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTA12730.2694407992210981No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTC12280.2599161833806036No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC9730.20594336028446855Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT6600.13969436566058502Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT6590.13948270753079625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG253.887305E-545.0000041
AATTACG302.1627766E-645.0000041
GTACGAG253.887305E-545.0000041
ACACGAC1050.045.026
TTTAACG207.029065E-445.026
TCTCGAG207.029065E-445.01
TTACGAG207.029065E-445.01
TAGTCAT207.029065E-445.025
AGCACGC207.029065E-445.025
CCGATGA8750.042.42857418
ACCACCG11350.042.22467414
CGAATAT750.042.014
GCTACGA750.042.010
CCACCGA11000.041.9318215
GATGAAT9200.041.57608420
TACGGGA1200.041.2500044
TTCGAAT603.6379788E-1241.25000443
AAACACG1150.041.08695640
AGACACG1150.041.08695624
CGATGAA9000.041.019
Produced by FastQC (version 0.11.5)