##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472460
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28335732125471	31.0	31.0	33.0	28.0	34.0
2	31.198433729839564	31.0	31.0	34.0	28.0	34.0
3	31.147108749947087	31.0	31.0	34.0	28.0	34.0
4	35.04163315412945	35.0	35.0	37.0	32.0	37.0
5	35.27065571688608	35.0	35.0	37.0	33.0	37.0
6	35.17614189561021	36.0	35.0	37.0	33.0	37.0
7	35.53141641620454	37.0	35.0	37.0	33.0	37.0
8	35.06919739237184	37.0	35.0	37.0	32.0	37.0
9	36.936377682766796	39.0	37.0	39.0	33.0	39.0
10	36.42142403589722	38.0	35.0	39.0	32.0	39.0
11	36.640695508614485	39.0	35.0	39.0	32.0	39.0
12	36.76011090886001	39.0	35.0	39.0	33.0	39.0
13	36.744526520763664	39.0	35.0	39.0	32.0	39.0
14	37.78081107395335	40.0	37.0	41.0	33.0	41.0
15	37.83350971510816	40.0	37.0	41.0	33.0	41.0
16	37.97016890318757	40.0	37.0	41.0	33.0	41.0
17	37.70746729881895	39.0	36.0	41.0	33.0	41.0
18	37.7045062015832	39.0	36.0	41.0	33.0	41.0
19	37.597233628243664	39.0	36.0	41.0	33.0	41.0
20	37.47971045167845	39.0	36.0	41.0	32.0	41.0
21	37.559873851754645	39.0	36.0	41.0	33.0	41.0
22	37.68799898404097	39.0	36.0	41.0	33.0	41.0
23	37.72962155526394	40.0	36.0	41.0	33.0	41.0
24	37.64704525250815	40.0	36.0	41.0	33.0	41.0
25	37.44215806629133	39.0	36.0	41.0	33.0	41.0
26	37.42540955848114	39.0	36.0	41.0	32.0	41.0
27	37.45198535325742	39.0	36.0	41.0	33.0	41.0
28	37.32100706938154	39.0	35.0	41.0	32.0	41.0
29	37.346001777928294	40.0	36.0	41.0	32.0	41.0
30	37.120304364390634	39.0	35.0	41.0	31.0	41.0
31	37.09179401430809	39.0	35.0	41.0	31.0	41.0
32	37.003896626169414	39.0	35.0	41.0	31.0	41.0
33	37.01266350590526	39.0	35.0	41.0	31.0	41.0
34	36.853454260678156	39.0	35.0	41.0	31.0	41.0
35	36.803308216568595	40.0	35.0	41.0	31.0	41.0
36	36.73803708250434	39.0	35.0	41.0	31.0	41.0
37	36.78391186555476	40.0	35.0	41.0	31.0	41.0
38	36.55993311603099	39.0	35.0	41.0	30.0	41.0
39	36.53585277060492	39.0	35.0	41.0	31.0	41.0
40	36.41847563814926	39.0	35.0	41.0	30.0	41.0
41	36.22516403505059	39.0	35.0	41.0	30.0	41.0
42	36.341899843372985	39.0	35.0	41.0	30.0	41.0
43	36.28527917707319	39.0	35.0	41.0	30.0	41.0
44	36.3078186513144	39.0	35.0	41.0	30.0	41.0
45	36.24928671210261	39.0	35.0	40.0	30.0	41.0
46	36.10787368242814	39.0	35.0	40.0	30.0	41.0
47	36.0122296067392	38.0	35.0	40.0	30.0	41.0
48	35.96962917495661	38.0	35.0	40.0	29.0	41.0
49	35.975551369428096	38.0	35.0	40.0	30.0	41.0
50	35.82065148372349	38.0	35.0	40.0	29.0	41.0
51	34.66640350505863	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	10.0
11	17.0
12	13.0
13	7.0
14	19.0
15	23.0
16	48.0
17	93.0
18	188.0
19	462.0
20	1026.0
21	1713.0
22	2350.0
23	2707.0
24	3042.0
25	3677.0
26	4273.0
27	4759.0
28	5230.0
29	5960.0
30	7495.0
31	9675.0
32	13202.0
33	18810.0
34	31768.0
35	35129.0
36	38519.0
37	58688.0
38	102532.0
39	120992.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.51064640392838	18.29615205520044	19.956398425263515	12.236803115607671
2	30.343097828387588	30.892139017059645	21.8067137958769	16.95804935867587
3	30.32531854548533	30.55750751386361	24.121407103246835	14.995766837404226
4	27.459255810015666	23.09084366930534	31.58722431528595	17.86267620539305
5	30.48469711721627	27.184946873809423	22.79558057825001	19.534775430724295
6	24.38428650044448	39.18278796088558	22.907547728908266	13.525377809761673
7	73.63586335351141	5.679634254751726	16.15713499555518	4.527367396181687
8	72.29077593870382	12.150658256783643	9.85268594166702	5.705879862845531
9	66.62997925750328	7.6033949964018115	11.311010455911612	14.455615290183296
10	41.498539558904454	24.42386657071498	19.03145239808661	15.04614147229395
11	33.017609956398424	23.26546162638107	23.77619269356136	19.940735723659145
12	26.680353892393004	20.90102865851077	28.143334885492955	24.275282563603266
13	25.573381873597768	22.771239893324303	32.34326715489142	19.312111078186515
14	20.36638022266435	30.50988443466113	27.071498116242644	22.052237226431867
15	19.027430893620622	24.92803623587182	34.86538542945434	21.17914744105321
16	20.78884984972273	25.372729966558015	28.158997587097325	25.679422596621936
17	20.389874275070905	26.969055581424882	29.488633958430345	23.152436185073867
18	21.725225415908227	25.389450958811327	28.34271684375397	24.54260678152648
19	22.838758836726917	26.812216907251408	25.411675062439148	24.937349193582527
20	26.756550819116963	25.454853320916055	29.107860982940352	18.680734877026627
21	23.879905177157852	29.529272319349786	26.847987131185707	19.74283537230665
22	21.47568048088727	23.782542437455024	29.394234432544554	25.34754264911315
23	23.445794352961098	27.95622909875968	27.82986919527579	20.76810735300343
24	23.209795538246624	25.424797866486053	26.635694027007577	24.729712568259746
25	21.5241501926089	31.397790289125005	24.6947889768446	22.383270541421496
26	21.92989882741396	25.18012106845024	28.33552046734115	24.554459636794647
27	25.189857342420524	26.670405960292936	25.12170342462854	23.018033272658002
28	19.638699572450577	28.693646022943742	28.781695804935868	22.885958599669813
29	25.393472463277316	23.99843372983956	27.558946789146173	23.049147017736953
30	22.361046437793675	27.361469754053253	27.112559793421664	23.164924014731405
31	23.543580408923507	28.249587266646913	23.43330652330356	24.77352580112602
32	25.132921305507345	27.75600050797951	27.424967192989886	19.68611099352326
33	24.606950852982262	25.156838674173475	25.152817169707486	25.083393303136774
34	24.30427972738433	27.27257333954197	26.53282817593024	21.89031875714346
35	22.361258095923464	23.739787495237692	28.593108411294075	25.30584599754477
36	23.763281547644244	28.84476992761292	26.322651653049995	21.069296871692842
37	20.811920585869704	26.517588790585446	29.66409854802523	23.00639207551962
38	22.083562629640603	28.048723701477375	25.814672141556954	24.053041527325064
39	24.14490115565339	25.168268213182067	29.17326334504508	21.513567286119457
40	24.41730516869153	23.522202937814843	30.37569318037506	21.68479871311857
41	19.432756212166108	28.516488168310545	28.268424840198115	23.782330779325235
42	20.686407314904965	24.7136265503958	31.990433052533547	22.609533082165687
43	24.145112813783175	23.186513143969858	28.61406256614317	24.0543114761038
44	22.983744655632226	23.78614062566143	28.284299199932267	24.945815518774076
45	22.105151758879057	22.675358760529992	27.15975955636456	28.05972992422639
46	25.47496084324599	25.570418659780724	26.858781695804936	22.09583880116835
47	21.186555475595817	24.375608517123144	32.76383185878169	21.674004148499343
48	21.802057317021546	25.57359353172755	27.567413114337725	25.05693603691318
49	20.648520509672778	23.466748507810184	33.09507683190112	22.789654150615927
50	21.82766795072599	22.90183295940397	30.335478135715192	24.935020954154847
51	21.480548617872415	22.686576641408795	27.05181391017229	28.7810608305465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	413.5
2	707.0
3	1685.0
4	2663.0
5	1864.0
6	1065.0
7	1041.0
8	1017.0
9	991.5
10	966.0
11	956.5
12	947.0
13	951.5
14	956.0
15	957.0
16	958.0
17	991.0
18	1024.0
19	1039.5
20	1055.0
21	1163.0
22	1271.0
23	1431.5
24	1592.0
25	1859.5
26	2795.0
27	3463.0
28	4237.5
29	5012.0
30	5488.5
31	5965.0
32	7137.5
33	8310.0
34	9254.0
35	10198.0
36	11375.5
37	12553.0
38	13644.0
39	14735.0
40	16892.0
41	19049.0
42	21894.0
43	24739.0
44	43910.0
45	63081.0
46	52648.5
47	42216.0
48	42384.5
49	42553.0
50	38532.5
51	34512.0
52	32417.5
53	30323.0
54	27881.0
55	25439.0
56	24356.0
57	23273.0
58	22697.5
59	22122.0
60	20967.5
61	19813.0
62	18241.5
63	16670.0
64	14384.5
65	12099.0
66	9603.0
67	7107.0
68	6026.5
69	4946.0
70	4128.0
71	3310.0
72	2565.5
73	1821.0
74	1467.5
75	947.0
76	780.0
77	663.5
78	547.0
79	351.5
80	156.0
81	100.5
82	45.0
83	35.0
84	25.0
85	16.5
86	8.0
87	6.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.61895606553551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61059809416366	23.68481909125781
2	9.082907337251804	5.925499107622934
3	3.3948272046953165	3.322071583201238
4	1.884692990967007	2.4590687147750203
5	1.415686176239265	2.3089102592667268
6	1.1970445823675548	2.3427806784440746
7	1.024206262610136	2.3385977357488446
8	0.9438098831571049	2.462887449033585
9	0.8666568173811826	2.544249658504826
>10	7.466838499762442	40.71758907326408
>50	0.0716794026271411	1.6056420392394923
>100	0.032581546648700506	2.0475443161231177
>500	0.0019548927989220303	0.4871765361314426
>1k	0.00456141653081807	2.540884953278911
>5k	0.0019548927989220303	5.212278804107868
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC	8768	1.8558184819878931	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	8496	1.798247470685349	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	7258	1.5362147060068576	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	3110	0.6582567836430597	Illumina PCR Primer Index 5 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1785	0.37780976167294583	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCATGATTT	1686	0.3568556068238581	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	1541	0.32616517800448713	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1331	0.28171697074884644	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTA	1273	0.2694407992210981	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTC	1228	0.2599161833806036	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	973	0.20594336028446855	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	660	0.13969436566058502	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	659	0.13948270753079625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.233162595775304E-4	0.0	0.0	0.1483723489819244	0.0
2	4.233162595775304E-4	0.0	0.0	1.1865554755958176	0.0
3	4.233162595775304E-4	0.0	0.0	1.4536680353892393	0.0
4	4.233162595775304E-4	0.0	0.0	2.1411336409431487	0.0
5	4.233162595775304E-4	0.0	0.0	4.907928713541887	0.0
6	4.233162595775304E-4	0.0	0.0	5.698895144562503	0.0
7	4.233162595775304E-4	0.0	0.0	6.579181306353977	0.0
8	4.233162595775304E-4	0.0	0.0	7.691444778393938	0.0
9	4.233162595775304E-4	0.0	0.0	8.002793887313212	0.0
10	4.233162595775304E-4	0.0	0.0	10.278753756931804	0.0
11	4.233162595775304E-4	0.0	0.0	11.332176268890487	0.0
12	4.233162595775304E-4	0.0	0.0	14.083731956144435	0.0
13	4.233162595775304E-4	0.0	0.0	14.516149515302883	0.0
14	4.233162595775304E-4	0.0	0.0	14.741777081657707	0.0
15	4.233162595775304E-4	0.0	0.0	15.297802988612792	0.0
16	4.233162595775304E-4	0.0	0.0	15.921771155230072	0.0
17	4.233162595775304E-4	0.0	0.0	16.587859289675315	0.0
18	4.233162595775304E-4	0.0	0.0	17.24738602209711	0.0
19	4.233162595775304E-4	0.0	0.0	18.060788214875334	0.0
20	4.233162595775304E-4	0.0	0.0	18.53066926300639	0.0
21	4.233162595775304E-4	0.0	0.0	19.050924946027177	0.0
22	4.233162595775304E-4	0.0	0.0	19.68272446344664	0.0
23	4.233162595775304E-4	0.0	0.0	20.220547771239893	0.0
24	6.349743893662956E-4	0.0	0.0	20.667781399483555	0.0
25	6.349743893662956E-4	0.0	0.0	21.05299919569911	0.0
26	6.349743893662956E-4	0.0	0.0	21.42445921347839	0.0
27	6.349743893662956E-4	0.0	0.0	21.838250857215424	0.0
28	6.349743893662956E-4	0.0	0.0	22.200397917284004	0.0
29	6.349743893662956E-4	0.0	0.0	22.589002243576175	0.0
30	8.466325191550608E-4	0.0	0.0	23.009143631206875	0.0
31	8.466325191550608E-4	0.0	0.0	23.41129407780553	0.0
32	8.466325191550608E-4	0.0	0.0	23.828472251619186	0.0
33	0.001058290648943826	0.0	0.0	24.220674766117767	0.0
34	0.001058290648943826	0.0	0.0	24.60187105786733	0.0
35	0.001058290648943826	0.0	0.0	24.99513186301486	0.0
36	0.001058290648943826	0.0	0.0	25.38606442873471	0.0
37	0.001058290648943826	0.0	0.0	25.768530669263008	0.0
38	0.001058290648943826	0.0	0.0	26.148245354104052	0.0
39	0.0012699487787325911	0.0	0.0	26.53409812470897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	25	3.887305E-5	45.000004	1
AATTACG	30	2.1627766E-6	45.000004	1
GTACGAG	25	3.887305E-5	45.000004	1
ACACGAC	105	0.0	45.0	26
TTTAACG	20	7.029065E-4	45.0	26
TCTCGAG	20	7.029065E-4	45.0	1
TTACGAG	20	7.029065E-4	45.0	1
TAGTCAT	20	7.029065E-4	45.0	25
AGCACGC	20	7.029065E-4	45.0	25
CCGATGA	875	0.0	42.428574	18
ACCACCG	1135	0.0	42.224674	14
CGAATAT	75	0.0	42.0	14
GCTACGA	75	0.0	42.0	10
CCACCGA	1100	0.0	41.93182	15
GATGAAT	920	0.0	41.576084	20
TACGGGA	120	0.0	41.250004	4
TTCGAAT	60	3.6379788E-12	41.250004	43
AAACACG	115	0.0	41.086956	40
AGACACG	115	0.0	41.086956	24
CGATGAA	900	0.0	41.0	19
>>END_MODULE