FastQCFastQC Report
Sat 18 Jun 2016
SRR3550834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456725
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC57681.2629043735289287No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC53341.1678800153265094No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG44700.9787070994580984No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC22100.48387979637637524No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.3227324976736548No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT13970.3058733373474191No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCGTAGT11460.2509168536865729No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC9960.21807433357052933No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT9870.21610378236356667No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTC8660.18961081613662487No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTA8060.17647380809020746No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT7960.1742843067491379No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6230.1364059335486343No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5590.12239312496578904No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT4860.10640976517598116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC600.045.00000433
ACGGGTC351.2097735E-745.0000045
TTACGTG302.1626183E-645.00000423
GCGTAAG302.1626183E-645.0000041
CTCGTAC207.0288166E-445.029
TTTACGA207.0288166E-445.045
TAACGTA207.0288166E-445.027
CGTTCGG207.0288166E-445.045
GCACCGA502.1827873E-1145.09
TCTACGG207.0288166E-445.02
CGTAGCA207.0288166E-445.016
CGTACAC207.0288166E-445.030
CGATGAA4250.042.8823519
CCGATGA4200.042.85714718
CGTAAGG650.041.5384642
TACGGCT5650.041.4159287
GGCCGAT501.0786607E-940.58
CGATAGG501.0786607E-940.52
ATAGGGC1250.039.64
TTGTGCG403.4521145E-739.3751