##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456725
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314147462915322	31.0	31.0	33.0	28.0	34.0
2	31.22950134106957	31.0	31.0	34.0	28.0	34.0
3	31.177270786578358	31.0	31.0	34.0	28.0	34.0
4	35.03222508073786	35.0	35.0	37.0	32.0	37.0
5	35.28942799277465	35.0	35.0	37.0	33.0	37.0
6	35.21180579123105	37.0	35.0	37.0	33.0	37.0
7	35.582490557775465	37.0	35.0	37.0	33.0	37.0
8	35.19424599047567	37.0	35.0	37.0	32.0	37.0
9	37.08907767256007	39.0	37.0	39.0	33.0	39.0
10	36.486997646286056	38.0	35.0	39.0	32.0	39.0
11	36.6618315178718	39.0	35.0	39.0	32.0	39.0
12	36.77599211779517	39.0	35.0	39.0	33.0	39.0
13	36.809489298812196	39.0	35.0	39.0	33.0	39.0
14	37.81507690623461	40.0	37.0	41.0	33.0	41.0
15	37.920639334391595	40.0	37.0	41.0	33.0	41.0
16	37.98036455197329	40.0	37.0	41.0	33.0	41.0
17	37.81675187476052	39.0	36.0	41.0	33.0	41.0
18	37.794434287591	39.0	36.0	41.0	33.0	41.0
19	37.67580491543051	39.0	36.0	41.0	33.0	41.0
20	37.663340084295804	39.0	36.0	41.0	33.0	41.0
21	37.64530735125075	39.0	36.0	41.0	33.0	41.0
22	37.794909409382015	40.0	36.0	41.0	33.0	41.0
23	37.84520663418906	40.0	36.0	41.0	33.0	41.0
24	37.74986260879085	40.0	36.0	41.0	33.0	41.0
25	37.48878208987903	39.0	36.0	41.0	33.0	41.0
26	37.540283540423665	39.0	36.0	41.0	33.0	41.0
27	37.580340467458534	40.0	36.0	41.0	33.0	41.0
28	37.49277792982648	40.0	36.0	41.0	33.0	41.0
29	37.520499206305765	40.0	36.0	41.0	33.0	41.0
30	37.27732661886255	39.0	35.0	41.0	32.0	41.0
31	37.2268586129509	39.0	35.0	41.0	32.0	41.0
32	37.14780666703158	39.0	35.0	41.0	32.0	41.0
33	37.16102030762494	39.0	35.0	41.0	32.0	41.0
34	37.071960151075594	39.0	35.0	41.0	32.0	41.0
35	36.980259455908914	39.0	35.0	41.0	32.0	41.0
36	36.84003941102414	39.0	35.0	41.0	31.0	41.0
37	36.807135584870544	39.0	35.0	41.0	31.0	41.0
38	36.676455197328806	39.0	35.0	41.0	31.0	41.0
39	36.653215830094695	39.0	35.0	41.0	31.0	41.0
40	36.52403525097159	39.0	35.0	41.0	31.0	41.0
41	36.350416552630136	39.0	35.0	41.0	30.0	41.0
42	36.40208878427938	39.0	35.0	41.0	30.0	41.0
43	36.3749433466528	39.0	35.0	41.0	31.0	41.0
44	36.314073019869724	39.0	35.0	40.0	31.0	41.0
45	36.32023865564618	39.0	35.0	40.0	30.0	41.0
46	36.1821774590837	39.0	35.0	40.0	30.0	41.0
47	36.088009195905634	38.0	35.0	40.0	30.0	41.0
48	36.010877442662434	38.0	35.0	40.0	30.0	41.0
49	36.01293995292572	38.0	35.0	40.0	30.0	41.0
50	35.865908369368874	38.0	35.0	40.0	29.0	41.0
51	34.67925775904538	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	18.0
10	21.0
11	16.0
12	20.0
13	25.0
14	31.0
15	33.0
16	34.0
17	92.0
18	178.0
19	452.0
20	755.0
21	1160.0
22	1664.0
23	2028.0
24	2518.0
25	3117.0
26	3867.0
27	4375.0
28	4919.0
29	5765.0
30	7233.0
31	9573.0
32	12645.0
33	17914.0
34	31283.0
35	34636.0
36	38319.0
37	58331.0
38	100995.0
39	114678.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.637965953254145	19.413213640593355	21.801740653566153	13.147079752586349
2	30.8292736329301	28.720564891345994	23.43883080628387	17.011330669440035
3	31.471454376265807	27.389785976243907	25.376101592862227	15.762658054628059
4	28.05079643111281	24.04532267776014	29.560238655646177	18.34364223548087
5	28.675461163719962	28.838579013629644	23.419782144616562	19.066177678033828
6	25.1801412228365	37.40894411297827	23.164267338113746	14.246647326071487
7	75.67376430018064	5.960260550659587	13.496743116755159	4.86923203240462
8	75.70376046855328	9.422737971427008	9.33625266845476	5.537248891564946
9	70.05594175926433	7.280091959056325	11.185067600853905	11.478898680825441
10	43.00968854343423	22.664185231813455	18.770813947123543	15.555312277628769
11	31.31556188078165	23.650555585965297	24.24303464885872	20.790847884394328
12	26.4800481690295	21.44047293228967	28.73260714872188	23.346871749958947
13	25.319393508128524	23.061360775083475	31.411680989654606	20.207564727133395
14	20.609557173353767	28.439870819420875	28.000437900268217	22.95013410695714
15	19.486780885653292	25.524549783786743	33.08358421369533	21.905085116864633
16	21.45623734194537	25.32026930866495	28.047293228967103	25.176200120422575
17	21.094531720400678	26.90612513000164	28.55525754009524	23.444085609502434
18	21.012644370244676	25.66949477256555	29.209918441075043	24.10794241611473
19	22.799715364825662	26.53872680497017	26.288028901417704	24.37352892878647
20	23.860090864305654	26.278395095517	29.720729103946574	20.140784936230773
21	24.046198478296567	28.448190924516943	27.78411516777054	19.72149542941595
22	22.350210739504078	23.659970441731897	28.648530297224806	25.34128852153922
23	22.778477201817285	26.741036728885	27.892495484153486	22.587990585144233
24	22.880726914445233	25.43696973014396	27.513273851880232	24.16902950353057
25	21.44200558322842	29.94471509113799	25.32421041107888	23.28906891455471
26	20.830696808801797	25.922382177459085	27.502326345174886	25.744594668564236
27	23.80338277957195	27.208495265203346	26.47479336581094	22.51332858941376
28	20.28923312715529	27.089386392249164	28.98658930428595	23.634791176309594
29	22.43516339153758	24.237998795774264	26.310799715364823	27.016038097323335
30	22.492747276807705	28.502052657507253	26.59105588702173	22.41414417866331
31	25.137226996551536	26.12140784936231	23.66894739723028	25.072417756855874
32	24.087142153374568	27.963435327604135	23.50517269691828	24.444249822103014
33	25.107230828178885	25.555202802561716	23.709672122174176	25.627894247085226
34	23.193825606218184	24.889813345010676	26.588209535278338	25.328151513492802
35	22.713449011987517	26.89473972302808	24.653785100443372	25.73802616454103
36	26.265477037604683	26.699436203404677	23.766599157041984	23.268487601948657
37	22.61470250150528	27.657780940390825	27.555969128031094	22.171547430072803
38	25.249548415348404	26.298538507854836	24.099622311018663	24.352290765778093
39	24.001970551206963	24.119765723356508	27.012534895177627	24.86572883025891
40	23.28775521375007	25.371065739777766	28.3461601620231	22.995018884449067
41	22.23504296896382	26.07958837374788	27.36898571350375	24.316382943784554
42	22.31255131643768	26.3005090590618	27.371832065247144	24.01510755925338
43	25.002572664075757	23.388910175707483	26.613826700968858	24.994690459247906
44	23.32300618534129	23.97263123323663	26.890798620614152	25.81356396080793
45	22.95801631178499	23.101647599759154	26.181400186107613	27.75893590234824
46	25.204663637856477	25.53637309102852	25.98434506541135	23.27461820570365
47	21.88756910613608	24.594668564234496	30.515737040888936	23.002025288740487
48	21.892823909354643	25.65263561223931	26.97531337237944	25.479227106026602
49	21.856916087361103	24.05605123433138	30.402320871421534	23.684711806885982
50	23.334829492583065	22.339482182932837	29.22196069845093	25.103727626033173
51	21.721167004214788	22.428813837648477	27.152006130603755	28.69801302753298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	461.0
2	740.0
3	1686.0
4	2632.0
5	1876.5
6	1121.0
7	1075.0
8	1029.0
9	1009.0
10	989.0
11	1015.0
12	1041.0
13	994.5
14	948.0
15	954.0
16	960.0
17	942.5
18	925.0
19	911.0
20	897.0
21	1029.5
22	1162.0
23	1311.5
24	1461.0
25	1710.0
26	2536.0
27	3113.0
28	3666.5
29	4220.0
30	5245.0
31	6270.0
32	7229.0
33	8188.0
34	9322.0
35	10456.0
36	11511.5
37	12567.0
38	13171.5
39	13776.0
40	15361.0
41	16946.0
42	18664.0
43	20382.0
44	22453.0
45	24524.0
46	27131.5
47	29739.0
48	43096.5
49	56454.0
50	49999.5
51	43545.0
52	42411.5
53	41278.0
54	36396.5
55	31515.0
56	28444.0
57	25373.0
58	23371.5
59	21370.0
60	20053.5
61	18737.0
62	16980.5
63	15224.0
64	13123.5
65	11023.0
66	9638.5
67	8254.0
68	7182.5
69	6111.0
70	5269.5
71	4428.0
72	3769.0
73	3110.0
74	2489.5
75	1537.0
76	1205.0
77	853.0
78	501.0
79	366.0
80	231.0
81	193.5
82	156.0
83	112.0
84	68.0
85	47.0
86	26.0
87	15.5
88	5.0
89	5.5
90	6.0
91	6.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	456725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.334801606132736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64464551343629	23.81285002030936
2	9.161706118697088	5.924838994435254
3	3.3549592220143776	3.2544582252150094
4	1.811174632655613	2.3425588968391833
5	1.2108149714646819	1.9575730942022875
6	0.9451731415296504	1.8337191608883876
7	0.8213704113038498	1.8591194506260338
8	0.6980689985812633	1.8057538061213518
9	0.6617290113481581	1.9257188669068546
>10	7.573541052464535	45.55193424014802
>50	0.06995432226719839	1.55614003974383
>100	0.03735424975432924	2.1510572522284526
>500	0.0047541772414600855	1.237050076753739
>1k	0.003395840886757204	2.3491433820406082
>5k	0.0013583363547028814	2.4380844935416315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	5768	1.2629043735289287	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC	5334	1.1678800153265094	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	4470	0.9787070994580984	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	2210	0.48387979637637524	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1474	0.3227324976736548	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	1397	0.3058733373474191	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCGTAGT	1146	0.2509168536865729	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	996	0.21807433357052933	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT	987	0.21610378236356667	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTC	866	0.18961081613662487	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTA	806	0.17647380809020746	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	796	0.1742843067491379	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	623	0.1364059335486343	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	559	0.12239312496578904	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	486	0.10640976517598116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1895013410695713E-4	0.0	0.0	0.18194756144288138	0.0
2	2.1895013410695713E-4	0.0	0.0	1.0492090426405387	0.0
3	2.1895013410695713E-4	0.0	0.0	1.3647161858886638	0.0
4	2.1895013410695713E-4	0.0	0.0	2.038644698669878	0.0
5	2.1895013410695713E-4	0.0	0.0	4.305654387213312	0.0
6	2.1895013410695713E-4	0.0	0.0	5.180360172970606	0.0
7	2.1895013410695713E-4	0.0	0.0	6.079150473479665	0.0
8	2.1895013410695713E-4	0.0	0.0	7.359351907603044	0.0
9	2.1895013410695713E-4	0.0	0.0	7.761782254091631	0.0
10	2.1895013410695713E-4	0.0	0.0	9.590672724287044	0.0
11	2.1895013410695713E-4	0.0	0.0	10.846132793256336	0.0
12	2.1895013410695713E-4	0.0	0.0	13.102413925228529	0.0
13	2.1895013410695713E-4	0.0	0.0	13.560019705512069	0.0
14	2.1895013410695713E-4	0.0	0.0	13.783348842301166	0.0
15	2.1895013410695713E-4	0.0	0.0	14.273359242432536	0.0
16	2.1895013410695713E-4	0.0	0.0	14.887076468334337	0.0
17	2.1895013410695713E-4	0.0	0.0	15.582462094258032	0.0
18	2.1895013410695713E-4	0.0	0.0	16.266024412939952	0.0
19	2.1895013410695713E-4	0.0	0.0	16.99512835951612	0.0
20	4.3790026821391426E-4	0.0	0.0	17.479665006294816	0.0
21	4.3790026821391426E-4	0.0	0.0	18.023756089550606	0.0
22	4.3790026821391426E-4	0.0	0.0	18.6466692210849	0.0
23	4.3790026821391426E-4	0.0	0.0	19.20345941211889	0.0
24	4.3790026821391426E-4	0.0	0.0	19.688652909299908	0.0
25	4.3790026821391426E-4	0.0	0.0	20.11801412228365	0.0
26	4.3790026821391426E-4	0.0	0.0	20.500738956702612	0.0
27	4.3790026821391426E-4	0.0	0.0	20.90382615359352	0.0
28	6.568504023208714E-4	0.0	0.0	21.295308993376757	0.0
29	6.568504023208714E-4	0.0	0.0	21.70386994362034	0.0
30	6.568504023208714E-4	0.0	0.0	22.156877771087636	0.0
31	6.568504023208714E-4	0.0	0.0	22.564125020526575	0.0
32	6.568504023208714E-4	0.0	0.0	22.961519513930703	0.0
33	6.568504023208714E-4	0.0	0.0	23.363074059882862	0.0
34	8.758005364278285E-4	0.0	0.0	23.757184301275384	0.0
35	8.758005364278285E-4	0.0	0.0	24.19070556680716	0.0
36	8.758005364278285E-4	0.0	0.0	24.57999890524933	0.0
37	8.758005364278285E-4	0.0	0.0	24.980896600799166	0.0
38	8.758005364278285E-4	0.0	0.0	25.35924243253599	0.0
39	8.758005364278285E-4	0.0	0.0	25.77152553505939	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	60	0.0	45.000004	33
ACGGGTC	35	1.2097735E-7	45.000004	5
TTACGTG	30	2.1626183E-6	45.000004	23
GCGTAAG	30	2.1626183E-6	45.000004	1
CTCGTAC	20	7.0288166E-4	45.0	29
TTTACGA	20	7.0288166E-4	45.0	45
TAACGTA	20	7.0288166E-4	45.0	27
CGTTCGG	20	7.0288166E-4	45.0	45
GCACCGA	50	2.1827873E-11	45.0	9
TCTACGG	20	7.0288166E-4	45.0	2
CGTAGCA	20	7.0288166E-4	45.0	16
CGTACAC	20	7.0288166E-4	45.0	30
CGATGAA	425	0.0	42.88235	19
CCGATGA	420	0.0	42.857147	18
CGTAAGG	65	0.0	41.538464	2
TACGGCT	565	0.0	41.415928	7
GGCCGAT	50	1.0786607E-9	40.5	8
CGATAGG	50	1.0786607E-9	40.5	2
ATAGGGC	125	0.0	39.6	4
TTGTGCG	40	3.4521145E-7	39.375	1
>>END_MODULE