FastQCFastQC Report
Sat 18 Jun 2016
SRR3550831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595008
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48170.8095689469721415No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC31460.5287323867914381No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC20480.34419705281273527TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC18510.31108825427557274No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT16810.28251720985264067No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG16280.27360976659137354TruSeq Adapter, Index 8 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG15610.2623494137893944No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC15250.25629907497042054No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC14120.23730773367753039TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT13150.2210054318597397TruSeq Adapter, Index 8 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT13050.2193247821878025No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT10730.18033370979885985TruSeq Adapter, Index 8 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT10210.17159433150478648TruSeq Adapter, Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.8885297E-545.01
CTATGCG253.8885297E-545.01
CGACGGT406.8048394E-945.028
CAACGAC207.030541E-445.011
CGCATCG207.030541E-445.021
AGTGCCG207.030541E-445.01
TCGTGGA207.030541E-445.031
ATAACGC207.030541E-445.011
TATCGAG253.8885297E-545.01
TATCCGG207.030541E-445.01
AACACGG207.030541E-445.02
CGTTTTT34200.043.4868431
TACGAGG603.6379788E-1241.2499962
GCGTTAG556.002665E-1140.909091
CGCTAGG1000.040.52
TCACGAC451.9255822E-839.99999625
CGGTCTA451.9255822E-839.99999631
CACGACG403.4544792E-739.37526
ACGATAG403.4544792E-739.3751
TTGCGAG800.039.3751
Produced by FastQC (version 0.11.5)