##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595008
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262835121544583	31.0	31.0	33.0	28.0	34.0
2	31.21911638162848	31.0	31.0	34.0	28.0	34.0
3	31.072041048187586	31.0	31.0	34.0	28.0	34.0
4	34.88736958158546	35.0	35.0	37.0	32.0	37.0
5	35.24645382919221	35.0	35.0	37.0	33.0	37.0
6	35.21711136656986	36.0	35.0	37.0	33.0	37.0
7	35.54741280789502	37.0	35.0	37.0	33.0	37.0
8	35.36947570452834	37.0	35.0	37.0	33.0	37.0
9	37.31786799505217	39.0	37.0	39.0	34.0	39.0
10	36.51200992255566	38.0	35.0	39.0	32.0	39.0
11	36.74210766914058	39.0	37.0	39.0	32.0	39.0
12	36.95004604980101	39.0	37.0	39.0	33.0	39.0
13	37.069694861245566	39.0	37.0	39.0	33.0	39.0
14	38.06978225502851	40.0	37.0	41.0	33.0	41.0
15	38.1981183446273	40.0	37.0	41.0	33.0	41.0
16	38.169848136495645	40.0	37.0	41.0	34.0	41.0
17	38.0701419140583	40.0	37.0	41.0	33.0	41.0
18	37.97597679358933	39.0	37.0	41.0	33.0	41.0
19	37.75312432774013	39.0	37.0	41.0	33.0	41.0
20	37.80467153382811	39.0	36.0	41.0	34.0	41.0
21	37.65413742336238	39.0	36.0	41.0	33.0	41.0
22	37.84540375927719	39.0	36.0	41.0	33.0	41.0
23	37.930740427019465	39.0	36.0	41.0	34.0	41.0
24	37.82463597128106	39.0	36.0	41.0	33.0	41.0
25	37.47021216521458	39.0	35.0	41.0	33.0	41.0
26	37.58693328493062	39.0	36.0	41.0	33.0	41.0
27	37.67853373400022	39.0	36.0	41.0	33.0	41.0
28	37.596017196407445	39.0	36.0	41.0	33.0	41.0
29	37.58454340109713	39.0	36.0	41.0	33.0	41.0
30	37.233233838872756	39.0	35.0	41.0	32.0	41.0
31	37.13738974938152	39.0	35.0	41.0	32.0	41.0
32	37.009388109067444	39.0	35.0	41.0	32.0	41.0
33	36.81559575669571	39.0	35.0	41.0	31.0	41.0
34	36.66507341077767	39.0	35.0	41.0	31.0	41.0
35	36.572404068516725	39.0	35.0	41.0	30.0	41.0
36	36.42813037807895	39.0	35.0	41.0	30.0	41.0
37	36.4543585968592	39.0	35.0	41.0	30.0	41.0
38	36.28155419759062	39.0	35.0	41.0	30.0	41.0
39	36.23469936538668	39.0	35.0	41.0	30.0	41.0
40	36.137498991610194	39.0	35.0	41.0	29.0	41.0
41	36.03903476928041	39.0	35.0	41.0	29.0	41.0
42	36.047095165107024	39.0	35.0	41.0	29.0	41.0
43	36.033856687641176	39.0	35.0	41.0	29.0	41.0
44	35.92280776056793	39.0	35.0	41.0	29.0	41.0
45	35.88433432827794	39.0	35.0	41.0	28.0	41.0
46	35.73713630741099	39.0	35.0	40.0	28.0	41.0
47	35.733275855114556	39.0	35.0	40.0	28.0	41.0
48	35.62208911476821	39.0	35.0	40.0	27.0	41.0
49	35.62456807303431	39.0	35.0	40.0	27.0	41.0
50	35.49995630310853	39.0	35.0	40.0	27.0	41.0
51	34.27848365063999	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	9.0
10	17.0
11	16.0
12	15.0
13	18.0
14	18.0
15	36.0
16	44.0
17	93.0
18	195.0
19	350.0
20	646.0
21	1105.0
22	1677.0
23	2553.0
24	3817.0
25	6264.0
26	8411.0
27	8843.0
28	8435.0
29	8443.0
30	9656.0
31	11663.0
32	15257.0
33	21318.0
34	35710.0
35	41776.0
36	48316.0
37	76076.0
38	138539.0
39	145655.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1412955792191	19.992336237495966	22.18457566957083	16.681792513714104
2	35.6430501774766	23.13750403355921	24.482527966010544	16.73691782295364
3	29.901278638270412	23.078681295041413	31.14479133053673	15.875248736151445
4	26.471408787781	23.533969291169193	31.876714262665374	18.117907658384425
5	24.19866623642035	28.400962676132085	29.93774873615145	17.462622351296115
6	24.146902226524684	33.61030439926858	28.4219707970313	13.820822577175432
7	75.38688555447995	5.220097881036893	14.273589598795311	5.119426965687857
8	76.46771135850274	5.00749569753684	13.03411046574164	5.49068247821878
9	71.18626976444014	6.0493304291707	15.227526352586857	7.536873453802302
10	37.095635688931914	22.506924276648384	24.042870011831774	16.35457002258793
11	26.523508927611054	24.509754490695922	28.807175701839306	20.159560879853718
12	23.718000430246317	21.533660051629557	33.447281381090676	21.30105813703345
13	23.12103366677423	22.360203560288262	35.66893218242444	18.84983059051307
14	20.054688340324837	26.34451973754975	32.550654781112186	21.05013714101323
15	18.988820318382274	26.625356297730452	35.66389023340862	18.721933150478648
16	20.876526029902116	26.058305098418842	32.382589813918464	20.682579057760567
17	21.156018070345272	26.756783102075936	30.823955308163924	21.263243519414864
18	21.37332607292675	25.826039313757125	32.2157013014951	20.58493331182102
19	21.21904243304292	29.41120119393353	29.045996020221576	20.32376035280198
20	21.295343928148867	27.337783693664626	31.322436000860492	20.04443637732602
21	21.351645692158762	28.07810987415295	31.715708024093793	18.854536409594495
22	20.330819081424114	24.384041895235022	31.32092341615575	23.964215607185114
23	19.538392761105733	26.964511401527375	31.114707701409056	22.382388135957836
24	20.715015596428955	25.732427127030228	31.257562923523718	22.294994353017103
25	20.24611433795848	28.218780251694092	29.22011132623427	22.314994084113156
26	19.275034957513178	27.324338496289123	30.516060288265034	22.88456625793267
27	20.80795552328708	27.40820291491879	29.625315962138327	22.158525599655803
28	19.282597881036896	26.487711089598797	31.184286597827253	23.045404431537055
29	20.990810207593846	24.84504410024739	29.396915671722063	24.7672300204367
30	21.170471657523933	27.861138001505864	28.784654996235343	22.183735344734863
31	22.01012423362375	27.14652576099817	26.833252662149082	24.010097343229
32	22.593477734753144	27.421816177261483	26.89778960955147	23.086916478433903
33	21.89701651070238	27.95508631816715	26.435442884801546	23.712454286328924
34	21.28878939442831	26.707372001720987	26.79207674518662	25.211761858664083
35	20.632663762504034	26.179816069699903	27.36080859417016	25.8267115736259
36	20.324936807572335	28.622136172959017	26.100153275250083	24.952773744218565
37	21.144253522641712	27.53509196515005	27.91777589545015	23.402878616758095
38	21.74256480585135	27.577276271915675	27.529041626331075	23.151117295901905
39	21.746262235129613	25.59797515327525	27.136273797999355	25.519488813595782
40	20.479388512423363	25.057142088845865	30.332869474023877	24.130599924706893
41	18.660757502420136	25.294449822523397	28.932888297300202	27.111904377756264
42	20.051999300849737	25.8080563622674	29.616912713778635	24.523031623104227
43	21.468114714424008	24.753784823061203	29.110532967623964	24.667567494890825
44	21.557189147036677	25.31377729375067	27.422824567064648	25.706208992148007
45	21.422905238248898	24.235472464235773	27.757105786812954	26.58451651070238
46	22.44591669355706	25.59343739916102	27.104005324298157	24.856640582983758
47	19.57704770356029	25.045377541142305	30.332701409056682	25.04487334624072
48	20.26140824997311	24.706054372378187	29.070197375497468	25.96234000215123
49	21.3560153813058	22.991119447133485	30.37488571582231	25.277979455738407
50	20.307794180918577	22.654317252877274	30.21841723136496	26.819471334839196
51	19.81855706141766	22.166760783048296	28.429365655587823	29.585316499946217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	265.0
1	472.5
2	680.0
3	4362.0
4	8044.0
5	5714.0
6	3384.0
7	3357.0
8	3330.0
9	3393.5
10	3457.0
11	3451.0
12	3445.0
13	3283.5
14	3122.0
15	3037.5
16	2953.0
17	2896.5
18	2840.0
19	2671.5
20	2503.0
21	2454.0
22	2405.0
23	2520.0
24	2635.0
25	2809.0
26	3353.5
27	3724.0
28	4556.0
29	5388.0
30	6262.5
31	7137.0
32	8431.0
33	9725.0
34	11600.0
35	13475.0
36	14905.0
37	16335.0
38	18700.5
39	21066.0
40	23440.5
41	25815.0
42	27551.0
43	29287.0
44	32502.5
45	35718.0
46	38849.0
47	41980.0
48	54160.5
49	66341.0
50	66939.5
51	67538.0
52	64684.5
53	61831.0
54	52143.0
55	42455.0
56	35406.5
57	28358.0
58	24149.0
59	19940.0
60	18157.0
61	16374.0
62	14557.0
63	12740.0
64	11054.5
65	9369.0
66	7626.5
67	5884.0
68	5085.0
69	4286.0
70	3714.0
71	3142.0
72	2500.0
73	1858.0
74	1593.0
75	1059.5
76	791.0
77	658.0
78	525.0
79	423.5
80	322.0
81	232.5
82	143.0
83	101.0
84	59.0
85	33.5
86	8.0
87	9.0
88	10.0
89	5.5
90	1.0
91	2.0
92	3.0
93	4.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.15062380292381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26948736416989	32.479253330902225
2	10.474296313373088	9.039448396374278
3	3.845637034545124	4.978249108807445
4	2.0982999049607	3.6217179929867984
5	1.3322197782929464	2.874305723796675
6	0.9752354526746752	2.524921088258341
7	0.7622490888676718	2.302406657449524
8	0.6176860118950108	2.1322829382087947
9	0.5260497021762787	2.0429435520223844
>10	4.015579798441367	30.196839212083688
>50	0.05394280749959937	1.5742104950854652
>100	0.023453394482910745	1.9319626016640385
>500	7.817798160970249E-4	0.1887433343165758
>1k	0.005081568804630661	4.11271556804385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4817	0.8095689469721415	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	3146	0.5287323867914381	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2048	0.34419705281273527	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	1851	0.31108825427557274	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1681	0.28251720985264067	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1628	0.27360976659137354	TruSeq Adapter, Index 8 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	1561	0.2623494137893944	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1525	0.25629907497042054	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1412	0.23730773367753039	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1315	0.2210054318597397	TruSeq Adapter, Index 8 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	1305	0.2193247821878025	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	1073	0.18033370979885985	TruSeq Adapter, Index 8 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	1021	0.17159433150478648	TruSeq Adapter, Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.361299343874368E-4	0.0	0.0	0.22974481015381307	0.0
2	3.361299343874368E-4	0.0	0.0	0.9836842529848339	0.0
3	3.361299343874368E-4	0.0	0.0	1.5174585887920835	0.0
4	3.361299343874368E-4	0.0	0.0	2.2779525653436594	0.0
5	3.361299343874368E-4	0.0	0.0	4.304984134667097	0.0
6	3.361299343874368E-4	0.0	0.0	5.806980746477358	0.0
7	3.361299343874368E-4	0.0	0.0	7.095534849951597	0.0
8	3.361299343874368E-4	0.0	0.0	9.043743949661181	0.0
9	3.361299343874368E-4	0.0	0.0	9.83011993116059	0.0
10	3.361299343874368E-4	0.0	0.0	11.613289233085942	0.0
11	3.361299343874368E-4	0.0	0.0	13.517633376357965	0.0
12	3.361299343874368E-4	0.0	0.0	15.331894697214155	0.0
13	3.361299343874368E-4	0.0	0.0	16.04902791222975	0.0
14	3.361299343874368E-4	0.0	0.0	16.40835081208992	0.0
15	3.361299343874368E-4	0.0	0.0	16.872714316446164	0.0
16	3.361299343874368E-4	0.0	0.0	17.756399913950737	0.0
17	3.361299343874368E-4	0.0	0.0	18.848654135742713	0.0
18	3.361299343874368E-4	0.0	0.0	19.975025545875013	0.0
19	3.361299343874368E-4	0.0	0.0	20.780560933634504	0.0
20	3.361299343874368E-4	0.0	0.0	21.498870603420457	0.0
21	3.361299343874368E-4	0.0	0.0	22.4449083037539	0.0
22	3.361299343874368E-4	0.0	0.0	23.422038023018178	0.0
23	3.361299343874368E-4	0.0	0.0	24.450259492309346	0.0
24	3.361299343874368E-4	0.0	0.0	25.2273919006131	0.0
25	3.361299343874368E-4	0.0	0.0	25.948558674841347	0.0
26	3.361299343874368E-4	0.0	0.0	26.585861030439926	0.0
27	3.361299343874368E-4	0.0	0.0	27.223835645907283	0.0
28	5.041949015811552E-4	0.0	0.0	27.89357454017425	0.0
29	6.722598687748736E-4	0.0	0.0	28.63121168118748	0.0
30	6.722598687748736E-4	0.0	0.0	29.408680219425623	0.0
31	6.722598687748736E-4	0.0	0.0	30.149678659782726	0.0
32	6.722598687748736E-4	0.0	0.0	30.857064106701085	0.0
33	6.722598687748736E-4	0.0	0.0	31.56411342368506	0.0
34	6.722598687748736E-4	0.0	0.0	32.179567333548455	0.0
35	8.40324835968592E-4	0.0	0.0	32.86107077551898	0.0
36	8.40324835968592E-4	0.0	0.0	33.51971738195117	0.0
37	8.40324835968592E-4	0.0	0.0	34.21483408626439	0.0
38	8.40324835968592E-4	0.0	0.0	34.88423685059697	0.0
39	8.40324835968592E-4	0.0	0.0	35.538513767882115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8885297E-5	45.0	1
CTATGCG	25	3.8885297E-5	45.0	1
CGACGGT	40	6.8048394E-9	45.0	28
CAACGAC	20	7.030541E-4	45.0	11
CGCATCG	20	7.030541E-4	45.0	21
AGTGCCG	20	7.030541E-4	45.0	1
TCGTGGA	20	7.030541E-4	45.0	31
ATAACGC	20	7.030541E-4	45.0	11
TATCGAG	25	3.8885297E-5	45.0	1
TATCCGG	20	7.030541E-4	45.0	1
AACACGG	20	7.030541E-4	45.0	2
CGTTTTT	3420	0.0	43.486843	1
TACGAGG	60	3.6379788E-12	41.249996	2
GCGTTAG	55	6.002665E-11	40.90909	1
CGCTAGG	100	0.0	40.5	2
TCACGAC	45	1.9255822E-8	39.999996	25
CGGTCTA	45	1.9255822E-8	39.999996	31
CACGACG	40	3.4544792E-7	39.375	26
ACGATAG	40	3.4544792E-7	39.375	1
TTGCGAG	80	0.0	39.375	1
>>END_MODULE