FastQCFastQC Report
Sat 18 Jun 2016
SRR3550829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466408
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC49971.0713795646729902No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC35350.7579201042863758No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG32400.6946707603643162No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC22760.48798476870036533TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.4766213272499614No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC16480.3533387077408621TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT13300.28515805903843844No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG10110.2167630057803468No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT9690.2077580144422909Illumina PCR Primer Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT7600.16294746230767912No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT6640.14236462496355123No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC6610.14172141129654722No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT6460.13850534296152725No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT6230.13357403818116326No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT5500.11792250561739935No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC5380.11534965094938338No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG207.0289697E-445.0000041
TTAGGCG253.8872287E-545.01
TTTCGAG253.8872287E-545.01
TCAAGCG351.2098462E-745.017
CTACGAA302.1627184E-644.99999611
AGCGAAG302.1627184E-644.9999961
TGCGTAG302.1627184E-644.9999961
CGTTTTT16000.042.8906251
TAACGTC556.002665E-1140.90909232
TACGGCT4100.039.5121967
TATTGCG403.4523327E-739.3750041
CCGATGA2600.038.9423118
GCGAACG356.2403087E-638.5714261
CGGTAGT356.2403087E-638.57142612
CGCCGGT356.2403087E-638.57142628
AGGGCGT356.2403087E-638.5714266
CGTTTCT1000.038.251
CGATGAA2650.038.20754219
AAGGGCC1250.037.8000035
CTATCGG301.13888214E-437.4999962
Produced by FastQC (version 0.11.5)