##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466408
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.357984854462188	31.0	31.0	33.0	28.0	34.0
2	31.306915833347627	31.0	31.0	34.0	28.0	34.0
3	31.193663058952676	31.0	31.0	34.0	28.0	34.0
4	35.021755630263634	35.0	35.0	37.0	32.0	37.0
5	35.31568497967445	35.0	35.0	37.0	33.0	37.0
6	35.26424075058747	37.0	35.0	37.0	33.0	37.0
7	35.62354419306701	37.0	35.0	37.0	35.0	37.0
8	35.348662544381746	37.0	35.0	37.0	33.0	37.0
9	37.25019082005454	39.0	37.0	39.0	34.0	39.0
10	36.553138025076755	38.0	35.0	39.0	32.0	39.0
11	36.7669315277611	39.0	37.0	39.0	33.0	39.0
12	36.933950532580916	39.0	37.0	39.0	33.0	39.0
13	37.018035711222794	39.0	37.0	39.0	33.0	39.0
14	38.04271153153462	40.0	37.0	41.0	33.0	41.0
15	38.150111061559834	40.0	37.0	41.0	33.0	41.0
16	38.17753554827533	40.0	37.0	41.0	34.0	41.0
17	38.05059733109209	40.0	37.0	41.0	33.0	41.0
18	38.00566456836075	39.0	37.0	41.0	33.0	41.0
19	37.8318467950807	39.0	37.0	41.0	33.0	41.0
20	37.89072228606713	39.0	36.0	41.0	33.0	41.0
21	37.82645880859676	39.0	36.0	41.0	33.0	41.0
22	38.00401365328211	40.0	37.0	41.0	34.0	41.0
23	38.07698838784926	40.0	37.0	41.0	34.0	41.0
24	37.98242097048078	40.0	37.0	41.0	34.0	41.0
25	37.66115932831341	39.0	36.0	41.0	33.0	41.0
26	37.761635735236105	40.0	36.0	41.0	33.0	41.0
27	37.831814634397354	40.0	36.0	41.0	33.0	41.0
28	37.74048472581946	40.0	36.0	41.0	33.0	41.0
29	37.74862137870706	40.0	36.0	41.0	33.0	41.0
30	37.48030694156189	40.0	36.0	41.0	33.0	41.0
31	37.410089449580624	39.0	36.0	41.0	33.0	41.0
32	37.31887317541723	40.0	36.0	41.0	32.0	41.0
33	37.252326289429	40.0	36.0	41.0	32.0	41.0
34	37.171377849436546	40.0	36.0	41.0	32.0	41.0
35	37.02136326992676	40.0	36.0	41.0	31.0	41.0
36	36.90841923809197	40.0	36.0	41.0	31.0	41.0
37	36.89975300595187	40.0	36.0	41.0	31.0	41.0
38	36.74509442376632	39.0	35.0	41.0	31.0	41.0
39	36.754000789008764	39.0	35.0	41.0	31.0	41.0
40	36.60287559390062	39.0	35.0	41.0	31.0	41.0
41	36.40505308656798	39.0	35.0	41.0	30.0	41.0
42	36.38407145675031	39.0	35.0	41.0	30.0	41.0
43	36.37428603282962	39.0	35.0	41.0	30.0	41.0
44	36.305910275981546	39.0	35.0	41.0	30.0	41.0
45	36.23534973671121	39.0	35.0	40.0	30.0	41.0
46	36.01695082417111	39.0	35.0	40.0	30.0	41.0
47	35.87219558841186	38.0	35.0	40.0	29.0	41.0
48	35.691223135109176	38.0	35.0	40.0	29.0	41.0
49	35.610594157904664	37.0	35.0	40.0	29.0	41.0
50	35.42485549132948	37.0	35.0	40.0	29.0	41.0
51	34.133314608668805	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	12.0
10	16.0
11	10.0
12	12.0
13	17.0
14	15.0
15	26.0
16	29.0
17	64.0
18	144.0
19	276.0
20	545.0
21	914.0
22	1215.0
23	1674.0
24	2445.0
25	3580.0
26	4797.0
27	5203.0
28	5462.0
29	6000.0
30	7069.0
31	9282.0
32	12432.0
33	16936.0
34	28338.0
35	33578.0
36	38883.0
37	62210.0
38	110312.0
39	114891.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16392514708153	19.98357661103583	23.0845096996621	13.767988542220543
2	32.964486029399154	25.889778906022194	24.165966278451485	16.979768786127167
3	30.294720502221228	25.739910121610265	27.665906245175897	16.29946313099261
4	27.169988507915814	24.441690537040532	30.325809162793092	18.062511792250564
5	26.346889418706372	28.245656163702165	26.64362532375088	18.763829093840588
6	24.41253151746968	35.62288811512667	25.596902282979713	14.367678084423938
7	76.59088180305656	5.249052331863948	13.444880876828872	4.7151849882506305
8	76.81171849539459	6.781830500334471	10.818853879007222	5.587597125263718
9	71.19689199156105	6.450361057271745	12.91573043344025	9.437016517726969
10	38.00535153770947	24.33598909109621	21.825740553335276	15.832918817859042
11	28.352858441536167	24.86942762559819	26.726814291350067	20.05089964151558
12	24.84348467436236	22.219172912986053	31.34873329788511	21.58860911476647
13	23.49831049210134	24.133591190545616	33.982050050599476	18.386048266753573
14	20.563755338673438	28.1442428088712	30.677218229532944	20.61478362292242
15	19.232517452530832	27.110812850551447	34.32552614877961	19.33114354813811
16	21.464683281590364	26.45516371931871	29.91479562957754	22.165357369513387
17	20.77344299411674	27.247388552511964	29.18067443097031	22.798494022400988
18	21.64456870379582	26.493113325671946	30.196523215725286	21.66579475480695
19	21.54680022641121	28.806324076773983	27.657544467504845	21.98933122930996
20	22.702011972350387	27.445498361949195	30.218178075847757	19.63431158985266
21	22.19301555719456	28.83140940978714	29.841469271539083	19.13410576147922
22	21.166017735544845	24.77294557554759	29.702749524021886	24.35828716488568
23	20.270235501963946	27.718864170425896	29.683667518567436	22.327232809042727
24	21.91321761204782	26.252337009656777	29.555882403389305	22.27856297490609
25	20.43489820071697	29.518147201591738	27.33229275655649	22.714661841134802
26	20.669885593729095	27.620023670262945	29.111207354933878	22.59888338107408
27	22.377832284180375	27.87387866417386	28.074132519167765	21.674156532478005
28	21.485909332601498	27.03984494262534	29.21390713709885	22.260338587674312
29	24.299969125743985	24.79224198555771	27.68048575496132	23.227303133736985
30	22.956295775372634	28.344925472976453	26.951295861134454	21.747482890516455
31	25.922797207595067	26.677501243546427	24.57783742988971	22.821864118968797
32	25.778288537074832	27.526757688547367	26.00191248863656	20.69304128574124
33	23.098017186669182	27.27054424452411	24.768443079878562	24.86299548892815
34	25.727903466492858	25.48648393681069	26.46652716076911	22.319085435927345
35	23.668547709301727	26.21374418963654	27.21887274660812	22.898835354453613
36	24.684610898612373	28.781453148316494	25.51006843793417	21.023867515136963
37	24.061980068952504	28.359290578206203	27.74073343510403	19.83799591773726
38	23.050419375310888	27.03898732440267	26.57372943860311	23.336863861683334
39	25.846683590332926	24.966338484760126	26.34345894581568	22.843518979091268
40	23.63938868973088	24.247440009605324	29.487272945575548	22.625898355088246
41	21.903355002487093	27.700425378638442	27.092374058764	23.30384556011046
42	22.293142484691515	26.82200991406665	29.55266633505429	21.332181266187543
43	24.10336014819643	26.54928731925696	27.290055059089894	22.057297473456718
44	23.511389169997084	25.095838836383592	26.769480797927997	24.623291195691326
45	23.89431570642013	23.296770209773417	26.361254523936122	26.447659559870328
46	25.766925095624433	25.198967427659902	26.33917085470232	22.694936622013344
47	21.77299703264095	24.957547897977737	31.180425721685733	22.08902934769558
48	22.132982281607518	25.207758014442287	28.179190751445088	24.480068952505103
49	22.420927599869643	23.549767585461655	30.58952676626473	23.439778048403973
50	22.42843175931802	22.828510660194507	29.963679868269843	24.779377712217627
51	21.92093617605187	22.56157698838785	27.118317009999828	28.399169825560456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	325.5
2	537.0
3	2192.0
4	3847.0
5	2713.0
6	1579.0
7	1593.0
8	1607.0
9	1611.5
10	1616.0
11	1582.0
12	1548.0
13	1492.0
14	1436.0
15	1411.5
16	1387.0
17	1376.0
18	1365.0
19	1355.0
20	1345.0
21	1444.5
22	1544.0
23	1644.0
24	1744.0
25	1920.0
26	2653.5
27	3211.0
28	3647.0
29	4083.0
30	5171.5
31	6260.0
32	7224.5
33	8189.0
34	9423.5
35	10658.0
36	12277.5
37	13897.0
38	15541.0
39	17185.0
40	18679.0
41	20173.0
42	21834.5
43	23496.0
44	25857.5
45	28219.0
46	30986.5
47	33754.0
48	45992.0
49	58230.0
50	53721.5
51	49213.0
52	48739.0
53	48265.0
54	40476.0
55	32687.0
56	27967.0
57	23247.0
58	20536.5
59	17826.0
60	15831.5
61	13837.0
62	12202.5
63	10568.0
64	9197.5
65	7827.0
66	6573.5
67	5320.0
68	4231.0
69	3142.0
70	2713.0
71	2284.0
72	1729.5
73	1175.0
74	1029.0
75	698.5
76	514.0
77	406.5
78	299.0
79	217.0
80	135.0
81	83.5
82	32.0
83	25.5
84	19.0
85	12.0
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.3079583322792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15120934562464	31.043430242694537
2	9.738862278474343	8.045850344060476
3	3.6128508195675266	4.477184733463084
4	2.0459318333514784	3.3805306769106584
5	1.4077539317605936	2.9075720377634413
6	1.0632801613339076	2.6353159559952095
7	0.8803204710705405	2.545496893662399
8	0.7554648195866819	2.496536743119158
9	0.6637287407341633	2.4675551249564665
>10	4.591982505721533	31.19614286457685
>50	0.058902973432533166	1.6599329699183478
>100	0.021371875316227116	1.6168681633306654
>500	0.004170122012922364	1.1651183422269584
>1k	0.004170122012922364	4.362464907321785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	4997	1.0713795646729902	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	3535	0.7579201042863758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	3240	0.6946707603643162	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2276	0.48798476870036533	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2223	0.4766213272499614	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1648	0.3533387077408621	TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1330	0.28515805903843844	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1011	0.2167630057803468	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	969	0.2077580144422909	Illumina PCR Primer Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	760	0.16294746230767912	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	664	0.14236462496355123	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	661	0.14172141129654722	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	646	0.13850534296152725	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	623	0.13357403818116326	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	550	0.11792250561739935	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	538	0.11534965094938338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2880911133599765E-4	0.0	0.0	0.2193358604483628	0.0
2	4.2880911133599765E-4	0.0	0.0	1.282568052005969	0.0
3	4.2880911133599765E-4	0.0	0.0	1.7977821992761702	0.0
4	4.2880911133599765E-4	0.0	0.0	2.592579887137442	0.0
5	4.2880911133599765E-4	0.0	0.0	5.073240596216189	0.0
6	4.2880911133599765E-4	0.0	0.0	6.405550505137133	0.0
7	4.2880911133599765E-4	0.0	0.0	7.555830946295947	0.0
8	4.2880911133599765E-4	0.0	0.0	9.176300578034683	0.0
9	4.2880911133599765E-4	0.0	0.0	9.7423286049982	0.0
10	4.2880911133599765E-4	0.0	0.0	11.60636181197578	0.0
11	4.2880911133599765E-4	0.0	0.0	13.296727328862284	0.0
12	4.2880911133599765E-4	0.0	0.0	15.352438208607056	0.0
13	4.2880911133599765E-4	0.0	0.0	15.96177595581551	0.0
14	4.2880911133599765E-4	0.0	0.0	16.22935284128917	0.0
15	4.2880911133599765E-4	0.0	0.0	16.711119877875166	0.0
16	4.2880911133599765E-4	0.0	0.0	17.588463319668616	0.0
17	4.2880911133599765E-4	0.0	0.0	18.64762182466853	0.0
18	4.2880911133599765E-4	0.0	0.0	19.691128797104682	0.0
19	4.2880911133599765E-4	0.0	0.0	20.487856125966964	0.0
20	4.2880911133599765E-4	0.0	0.0	21.150151798425412	0.0
21	4.2880911133599765E-4	0.0	0.0	21.9368021131713	0.0
22	4.2880911133599765E-4	0.0	0.0	22.798708426956654	0.0
23	4.2880911133599765E-4	0.0	0.0	23.70499648376529	0.0
24	4.2880911133599765E-4	0.0	0.0	24.392806298348226	0.0
25	4.2880911133599765E-4	0.0	0.0	25.010077014116398	0.0
26	4.2880911133599765E-4	0.0	0.0	25.585324436973636	0.0
27	4.2880911133599765E-4	0.0	0.0	26.18608600195537	0.0
28	4.2880911133599765E-4	0.0	0.0	26.778056980154716	0.0
29	4.2880911133599765E-4	0.0	0.0	27.376674499579767	0.0
30	4.2880911133599765E-4	0.0	0.0	28.049690399821614	0.0
31	6.432136670039965E-4	0.0	0.0	28.662887429032093	0.0
32	6.432136670039965E-4	0.0	0.0	29.31467727826281	0.0
33	6.432136670039965E-4	0.0	0.0	29.89635683779009	0.0
34	6.432136670039965E-4	0.0	0.0	30.46410010119895	0.0
35	6.432136670039965E-4	0.0	0.0	31.05864393406631	0.0
36	6.432136670039965E-4	0.0	0.0	31.627244815697843	0.0
37	6.432136670039965E-4	0.0	0.0	32.20270664311075	0.0
38	6.432136670039965E-4	0.0	0.0	32.794892025865764	0.0
39	6.432136670039965E-4	0.0	0.0	33.3748563489477	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGACG	20	7.0289697E-4	45.000004	1
TTAGGCG	25	3.8872287E-5	45.0	1
TTTCGAG	25	3.8872287E-5	45.0	1
TCAAGCG	35	1.2098462E-7	45.0	17
CTACGAA	30	2.1627184E-6	44.999996	11
AGCGAAG	30	2.1627184E-6	44.999996	1
TGCGTAG	30	2.1627184E-6	44.999996	1
CGTTTTT	1600	0.0	42.890625	1
TAACGTC	55	6.002665E-11	40.909092	32
TACGGCT	410	0.0	39.512196	7
TATTGCG	40	3.4523327E-7	39.375004	1
CCGATGA	260	0.0	38.94231	18
GCGAACG	35	6.2403087E-6	38.571426	1
CGGTAGT	35	6.2403087E-6	38.571426	12
CGCCGGT	35	6.2403087E-6	38.571426	28
AGGGCGT	35	6.2403087E-6	38.571426	6
CGTTTCT	100	0.0	38.25	1
CGATGAA	265	0.0	38.207542	19
AAGGGCC	125	0.0	37.800003	5
CTATCGG	30	1.13888214E-4	37.499996	2
>>END_MODULE