SRR3550823_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292125
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21343	7.306118955926401	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	693	0.23722721437740693	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	643	0.2201112537441164	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	587	0.20094137783483096	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	571	0.195464270432178	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	531	0.18177150192554556	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	451	0.1543859649122807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	398	0.1362430466409927	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	381	0.13042362002567395	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	366	0.1252888318356868	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	304	0.10406504065040649	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCT	20	7.024638E-4	45.000004	20
CGTTAGG	20	7.024638E-4	45.000004	2
ACGGCGC	20	7.024638E-4	45.000004	8
CGATGCT	20	7.024638E-4	45.000004	10
TCTAGCG	25	3.88364E-5	45.0	1
ATACTGG	25	3.88364E-5	45.0	2
TAGGGCC	25	3.88364E-5	45.0	5
CGGTAGT	35	1.2077726E-7	45.0	12
AATGCGG	30	2.1599244E-6	44.999996	2
CGTTTTT	11205	0.0	44.457832	1
ACGGGAG	135	0.0	43.333336	5
ATGGGAC	115	0.0	41.08696	5
GTTTTTT	12280	0.0	40.6759	2
GCGATGC	50	1.0750227E-9	40.5	9
ATGGTCA	50	1.0750227E-9	40.5	13
TACGGGA	80	0.0	39.375004	4
GCCGATC	35	6.2323015E-6	38.571426	9
ATTGGGC	35	6.2323015E-6	38.571426	4
CGCCGGT	35	6.2323015E-6	38.571426	28
GACCGAT	90	0.0	37.5	9