##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292125
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.464845528455285	34.0	31.0	34.0	31.0	34.0
2	32.66175438596491	34.0	31.0	34.0	31.0	34.0
3	32.49943688489517	34.0	31.0	34.0	31.0	34.0
4	36.11086350021395	37.0	35.0	37.0	35.0	37.0
5	36.1442053915276	37.0	35.0	37.0	35.0	37.0
6	36.16199229781771	37.0	37.0	37.0	35.0	37.0
7	36.299765511339324	37.0	37.0	37.0	35.0	37.0
8	36.07121951219512	37.0	37.0	37.0	35.0	37.0
9	37.911986307231494	39.0	39.0	39.0	35.0	39.0
10	37.8002190842961	39.0	38.0	39.0	35.0	39.0
11	37.774795036371415	39.0	38.0	39.0	35.0	39.0
12	37.643652545999146	39.0	37.0	39.0	35.0	39.0
13	37.62998031664527	39.0	38.0	39.0	35.0	39.0
14	39.04802053915276	41.0	39.0	41.0	35.0	41.0
15	39.14278476679504	41.0	39.0	41.0	35.0	41.0
16	39.17653401797176	41.0	39.0	41.0	35.0	41.0
17	39.09500385109114	41.0	39.0	41.0	35.0	41.0
18	38.412042789901584	39.0	38.0	40.0	35.0	41.0
19	37.710846384253315	37.0	37.0	40.0	35.0	41.0
20	36.85323063756953	35.0	35.0	40.0	34.0	41.0
21	36.83217458279846	35.0	35.0	40.0	34.0	41.0
22	36.82960034231921	35.0	35.0	40.0	34.0	41.0
23	36.82309285408643	35.0	35.0	40.0	34.0	41.0
24	36.666382541720154	35.0	35.0	40.0	34.0	41.0
25	36.5972135216089	35.0	35.0	40.0	34.0	41.0
26	36.44124946512623	35.0	35.0	40.0	33.0	41.0
27	36.356717158750534	35.0	35.0	40.0	33.0	41.0
28	36.19696020539153	35.0	35.0	40.0	33.0	41.0
29	35.94190500641849	35.0	35.0	40.0	33.0	41.0
30	35.41724946512623	35.0	35.0	40.0	31.0	41.0
31	34.73544886606761	35.0	35.0	40.0	24.0	41.0
32	33.649899871630296	35.0	34.0	40.0	18.0	41.0
33	32.446291827128796	35.0	33.0	40.0	12.0	41.0
34	31.389918699186993	35.0	28.0	40.0	8.0	41.0
35	30.816065040650408	35.0	23.0	40.0	7.0	41.0
36	30.444121523320497	35.0	22.0	40.0	7.0	41.0
37	30.257204963628585	35.0	22.0	40.0	7.0	41.0
38	30.122125802310656	35.0	21.0	40.0	7.0	41.0
39	30.0139323919555	35.0	21.0	40.0	7.0	41.0
40	29.902343175010696	35.0	21.0	40.0	7.0	41.0
41	29.74522892597347	35.0	20.0	40.0	7.0	41.0
42	29.638702610183998	35.0	20.0	40.0	7.0	41.0
43	29.491125374411638	35.0	18.0	40.0	7.0	41.0
44	29.349110825845102	35.0	18.0	40.0	7.0	41.0
45	29.267594351732992	35.0	20.0	40.0	7.0	41.0
46	29.214281557552418	35.0	20.0	40.0	7.0	41.0
47	29.208633290543432	35.0	20.0	40.0	7.0	41.0
48	29.171977749251177	35.0	20.0	40.0	7.0	41.0
49	29.155847667950365	35.0	20.0	40.0	7.0	41.0
50	29.053774925117672	35.0	20.0	40.0	7.0	41.0
51	28.46845699614891	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	4.0
11	4.0
12	2.0
13	2.0
14	5.0
15	9.0
16	16.0
17	63.0
18	117.0
19	281.0
20	473.0
21	768.0
22	1270.0
23	2225.0
24	4389.0
25	8931.0
26	15177.0
27	18650.0
28	17395.0
29	13629.0
30	10575.0
31	9064.0
32	8285.0
33	8550.0
34	14012.0
35	21121.0
36	20021.0
37	18013.0
38	33238.0
39	65758.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.02481814291827	12.250235344458709	14.609499358151476	47.115447154471546
2	59.32973898160034	14.994608472400515	14.877192982456142	10.798459563543004
3	19.503637141634574	15.838767650834404	54.30415062045357	10.35344458707745
4	17.4808729139923	15.44201968335473	55.064099272571674	12.013008130081301
5	16.223534445870776	17.643474540008558	53.75404364569961	12.378947368421054
6	15.292768506632434	21.23337612323492	53.71125374411639	9.76260162601626
7	48.421394950791615	3.6693196405648267	44.59940094137784	3.309884467265725
8	48.544629867351304	4.027385537013265	43.865810868635	3.5621737270004274
9	45.154985023534444	4.5887890457851945	44.95507060333761	5.301155327342747
10	25.006760804450153	17.976208814719726	48.99717586649551	8.019854514334616
11	20.302952503209244	14.420881471972615	53.52982456140351	11.746341463414634
12	18.22267864783911	13.459649122807019	55.734702610183994	12.582969619169877
13	15.962344886606761	14.960034231921265	56.497047496790756	12.580573384681216
14	12.284809584937955	18.04775353016688	55.95926401369277	13.708172871202395
15	12.527513906718015	15.653915275994864	59.17740693196406	12.641163885323065
16	14.227813436029097	15.676166024818142	57.466837826273	12.62918271287976
17	13.600342319212666	15.54163457424048	55.960975609756105	14.897047496790758
18	14.954899443731279	15.676166024818142	55.73230637569534	13.636628155755243
19	15.20239623448866	17.074197689345315	54.20522036799316	13.518185708172872
20	16.938296961916986	15.998973042362003	55.036029097133074	12.026700898587933
21	15.164741121095421	17.287120239623448	55.02473256311511	12.523406076166024
22	14.359948652118101	15.38451005562687	55.760718870346594	14.49482242190843
23	14.229525032092427	16.47616602481814	55.92913992297818	13.365169020111253
24	15.318784766795037	14.423277706461274	56.0379974326059	14.219940094137783
25	13.651690201112537	16.392640136927685	54.96756525459992	14.988104407359861
26	13.01668806161746	16.425160462130936	56.305348737697905	14.252802738553703
27	14.515019255455714	16.17869062901155	55.26949080017116	14.036799315361575
28	12.280701754385964	17.326486949080017	56.1612323491656	14.23157894736842
29	14.394522892597347	15.750449293966623	56.13795464270432	13.717073170731709
30	15.371501925545573	16.16260162601626	54.84124946512623	13.624646983311939
31	15.837740693196405	17.47163029525032	52.73016688061617	13.960462130937099
32	17.247411210954215	18.481471972614465	50.614291827128795	13.656824989302525
33	17.027299957210097	20.11844244758237	46.76285836542576	16.09139922978177
34	15.59298245614035	22.635515618314077	46.46161745827985	15.309884467265725
35	16.77535301668806	22.81694480102696	43.43585793752675	16.971844244758234
36	17.884809584937955	25.69002995293111	41.16525459991442	15.259905862216517
37	18.891570389388104	24.925288831835687	41.1864783910997	14.996662387676507
38	17.604450149764656	25.343945228925975	39.91510483525888	17.13649978605049
39	19.292768506632434	23.92811296534018	39.402652973898164	17.376465554129226
40	18.0936243046641	23.724433033804022	40.983825417201544	17.198117244330337
41	16.934531450577666	24.989644843816862	40.04176294394523	18.034060761660246
42	18.43765511339324	23.67787762088147	40.17629439452289	17.708172871202397
43	19.517329910141207	22.93846812152332	39.47145913564399	18.072742832691485
44	18.07548138639281	23.57894736842105	39.261617458279844	19.083953786906292
45	17.921780059905863	22.78271287976038	39.78262729995721	19.51287976037655
46	17.925545571245184	23.829525032092427	40.048267008985874	18.196662387676508
47	16.600770218228497	23.297903294822422	42.628326914848095	17.472999572100985
48	17.230295250320925	21.639366709456567	42.558836114676936	18.571501925545572
49	17.598630723149338	20.84997860504921	43.022336328626444	18.529054343175012
50	16.26974753958066	21.194009413778346	43.0938810440736	19.442362002567396
51	15.957210098416773	21.06324347454001	41.623620025673944	21.355926401369278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	159.0
2	267.0
3	19591.0
4	38915.0
5	25363.0
6	11811.0
7	11180.0
8	10549.0
9	10257.5
10	9966.0
11	9436.5
12	8907.0
13	8271.5
14	7636.0
15	6965.0
16	6294.0
17	5756.0
18	5218.0
19	4726.0
20	4234.0
21	3844.0
22	3454.0
23	3114.5
24	2775.0
25	2697.5
26	2533.0
27	2446.0
28	2687.0
29	2928.0
30	3215.5
31	3503.0
32	3731.0
33	3959.0
34	4431.5
35	4904.0
36	5140.0
37	5376.0
38	5865.5
39	6355.0
40	6972.0
41	7589.0
42	8649.0
43	9709.0
44	10819.5
45	11930.0
46	14239.5
47	16549.0
48	17157.5
49	17766.0
50	17823.0
51	17880.0
52	16071.0
53	14262.0
54	12692.5
55	11123.0
56	10237.0
57	9351.0
58	8885.5
59	8420.0
60	8112.5
61	7805.0
62	6968.0
63	6131.0
64	5237.0
65	4343.0
66	3481.0
67	2619.0
68	2207.0
69	1795.0
70	1488.0
71	1181.0
72	921.0
73	661.0
74	524.0
75	311.5
76	236.0
77	179.5
78	123.0
79	85.5
80	48.0
81	33.0
82	18.0
83	17.5
84	17.0
85	14.5
86	12.0
87	6.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	292125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.19785014899649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6218734667726	37.24557229149317
2	7.239817283627299	6.689279879502576
3	2.620394265396782	3.6316974481227073
4	1.6824292208393314	3.108984521225134
5	1.2622087803281694	2.9155666045174202
6	1.0042701430851328	2.7837072947614936
7	0.859980847240985	2.7810486418292246
8	0.7838936820198896	2.8971362283759974
9	0.6349709419914553	2.6400863184379557
>10	3.168083193182019	21.659071452182243
>50	0.08858111516126273	2.728744534750573
>100	0.029030785641086108	2.5392627151352527
>500	0.0037218955950110394	1.0402586285596074
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	7.443791190022079E-4	7.339583441106677
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21343	7.306118955926401	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	693	0.23722721437740693	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	643	0.2201112537441164	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	587	0.20094137783483096	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	571	0.195464270432178	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	531	0.18177150192554556	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	451	0.1543859649122807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	398	0.1362430466409927	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	381	0.13042362002567395	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	366	0.1252888318356868	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	304	0.10406504065040649	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1297389816003423	0.0
2	0.0	0.0	0.0	0.7065468549422337	0.0
3	0.0	0.0	0.0	0.9838254172015405	0.0
4	0.0	0.0	0.0	1.2925973470261019	0.0
5	3.4231921266581085E-4	0.0	0.0	2.3212665810868636	0.0
6	3.4231921266581085E-4	0.0	0.0	2.765939238339752	0.0
7	3.4231921266581085E-4	0.0	0.0	3.240393667094566	0.0
8	3.4231921266581085E-4	0.0	0.0	3.9551561831407787	0.0
9	3.4231921266581085E-4	0.0	0.0	4.2098416773641425	0.0
10	3.4231921266581085E-4	0.0	0.0	4.945827984595636	0.0
11	3.4231921266581085E-4	0.0	0.0	5.779717586649551	0.0
12	3.4231921266581085E-4	0.0	0.0	6.66153187847668	0.0
13	3.4231921266581085E-4	0.0	0.0	6.9442875481386395	0.0
14	3.4231921266581085E-4	0.0	0.0	7.064441591784339	0.0
15	3.4231921266581085E-4	0.0	0.0	7.305776636713736	0.0
16	3.4231921266581085E-4	0.0	0.0	7.754557124518613	0.0
17	3.4231921266581085E-4	0.0	0.0	8.266666666666667	0.0
18	3.4231921266581085E-4	0.0	0.0	8.825673940949935	0.0
19	6.846384253316217E-4	0.0	0.0	9.212836970474967	0.0
20	6.846384253316217E-4	0.0	0.0	9.565083440308086	0.0
21	6.846384253316217E-4	0.0	0.0	9.997774925117673	0.0
22	6.846384253316217E-4	0.0	0.0	10.432520325203251	0.0
23	6.846384253316217E-4	0.0	0.0	10.864527171587506	0.0
24	6.846384253316217E-4	0.0	0.0	11.221908429610611	0.0
25	6.846384253316217E-4	0.0	0.0	11.53170731707317	0.0
26	6.846384253316217E-4	0.0	0.0	11.83157894736842	0.0
27	6.846384253316217E-4	0.0	0.0	12.134531450577663	0.0
28	6.846384253316217E-4	0.0	0.0	12.42892597347026	0.0
29	6.846384253316217E-4	0.0	0.0	12.71099700470689	0.0
30	6.846384253316217E-4	0.0	0.0	13.050577663671374	0.0
31	6.846384253316217E-4	0.0	0.0	13.380915703893882	0.0
32	6.846384253316217E-4	0.0	0.0	13.688318356867779	0.0
33	6.846384253316217E-4	0.0	0.0	13.978605049208387	0.0
34	6.846384253316217E-4	0.0	0.0	14.285323063756953	0.0
35	6.846384253316217E-4	0.0	0.0	14.601626016260163	0.0
36	6.846384253316217E-4	0.0	0.0	14.903209242618741	0.0
37	6.846384253316217E-4	0.0	0.0	15.214377406931964	0.0
38	6.846384253316217E-4	0.0	0.0	15.533076593923834	0.0
39	6.846384253316217E-4	0.0	0.0	15.864441591784338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCT	20	7.024638E-4	45.000004	20
CGTTAGG	20	7.024638E-4	45.000004	2
ACGGCGC	20	7.024638E-4	45.000004	8
CGATGCT	20	7.024638E-4	45.000004	10
TCTAGCG	25	3.88364E-5	45.0	1
ATACTGG	25	3.88364E-5	45.0	2
TAGGGCC	25	3.88364E-5	45.0	5
CGGTAGT	35	1.2077726E-7	45.0	12
AATGCGG	30	2.1599244E-6	44.999996	2
CGTTTTT	11205	0.0	44.457832	1
ACGGGAG	135	0.0	43.333336	5
ATGGGAC	115	0.0	41.08696	5
GTTTTTT	12280	0.0	40.6759	2
GCGATGC	50	1.0750227E-9	40.5	9
ATGGTCA	50	1.0750227E-9	40.5	13
TACGGGA	80	0.0	39.375004	4
GCCGATC	35	6.2323015E-6	38.571426	9
ATTGGGC	35	6.2323015E-6	38.571426	4
CGCCGGT	35	6.2323015E-6	38.571426	28
GACCGAT	90	0.0	37.5	9
>>END_MODULE