##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382933
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26336460947476	33.0	31.0	34.0	30.0	34.0
2	32.478739100573726	34.0	31.0	34.0	30.0	34.0
3	32.34989932964775	34.0	31.0	34.0	30.0	34.0
4	36.00858635844913	37.0	35.0	37.0	35.0	37.0
5	35.992113502884315	37.0	35.0	37.0	35.0	37.0
6	36.000201079562224	37.0	35.0	37.0	35.0	37.0
7	36.11657130620761	37.0	35.0	37.0	35.0	37.0
8	35.644303311545364	37.0	35.0	37.0	33.0	37.0
9	37.513121094290646	39.0	37.0	39.0	35.0	39.0
10	37.50661603988165	39.0	37.0	39.0	34.0	39.0
11	37.51240817584277	39.0	37.0	39.0	35.0	39.0
12	37.42321763859474	39.0	37.0	39.0	35.0	39.0
13	37.31948147587176	39.0	37.0	39.0	34.0	39.0
14	38.74688000250696	40.0	38.0	41.0	34.0	41.0
15	38.78322317481126	41.0	38.0	41.0	34.0	41.0
16	38.933346042258044	41.0	38.0	41.0	35.0	41.0
17	38.743477318486526	41.0	38.0	41.0	34.0	41.0
18	38.216434728790674	39.0	38.0	40.0	35.0	41.0
19	37.544607020027	37.0	37.0	40.0	34.0	41.0
20	36.44243509961272	35.0	35.0	40.0	33.0	41.0
21	36.63638286593216	36.0	35.0	40.0	33.0	41.0
22	36.551062457401166	35.0	35.0	40.0	33.0	41.0
23	36.350980458722546	35.0	35.0	40.0	33.0	41.0
24	36.36311574087373	35.0	35.0	40.0	33.0	41.0
25	36.45085171557427	35.0	35.0	40.0	33.0	41.0
26	36.09451522851256	35.0	35.0	40.0	33.0	41.0
27	35.87332771006938	35.0	35.0	40.0	32.0	41.0
28	35.74763470372102	35.0	35.0	40.0	31.0	41.0
29	35.501777073273914	35.0	35.0	40.0	30.0	41.0
30	35.05892675742232	35.0	35.0	40.0	27.0	41.0
31	34.45752128962508	35.0	35.0	40.0	23.0	41.0
32	33.28514648776679	35.0	33.0	40.0	16.0	41.0
33	32.21881373503981	35.0	31.0	40.0	12.0	41.0
34	31.142803049097363	35.0	26.0	40.0	8.0	41.0
35	30.52853110074086	35.0	23.0	40.0	7.0	41.0
36	30.223075577189743	35.0	22.0	40.0	7.0	41.0
37	29.877312741393403	35.0	20.0	40.0	7.0	41.0
38	29.767356169355995	35.0	20.0	40.0	7.0	41.0
39	29.585475788192714	35.0	18.0	40.0	7.0	41.0
40	29.514076875066916	35.0	18.0	40.0	7.0	41.0
41	29.40448590223355	35.0	19.0	40.0	7.0	41.0
42	29.23140862761893	35.0	18.0	40.0	7.0	41.0
43	28.947009006797533	35.0	18.0	40.0	7.0	41.0
44	29.02157818730692	35.0	18.0	40.0	7.0	41.0
45	28.970848686323716	35.0	18.0	40.0	7.0	41.0
46	28.92535769964981	35.0	18.0	40.0	7.0	41.0
47	28.777666066909877	35.0	18.0	40.0	7.0	41.0
48	28.813445171870796	35.0	18.0	40.0	7.0	41.0
49	28.767839282589904	35.0	18.0	39.0	7.0	41.0
50	28.567796455254573	35.0	18.0	39.0	7.0	41.0
51	28.052027378157536	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	6.0
12	10.0
13	5.0
14	16.0
15	21.0
16	42.0
17	104.0
18	253.0
19	554.0
20	1209.0
21	2450.0
22	3658.0
23	5136.0
24	7535.0
25	12434.0
26	19863.0
27	24474.0
28	21912.0
29	16872.0
30	13180.0
31	11580.0
32	11048.0
33	11942.0
34	17213.0
35	25923.0
36	26714.0
37	25212.0
38	43840.0
39	79634.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.64948959739693	11.066687906239473	12.332966863654999	42.950855632708596
2	54.14445869120708	23.3286240673956	12.703266628888082	9.823650612509239
3	17.853514844633395	23.798680186873423	48.892626125196834	9.455178843296347
4	16.00645543737416	14.193344527632771	59.03199776462201	10.768202270371056
5	22.776308127009163	16.504453781732053	48.296960564902996	12.422277526355785
6	14.382672686866893	27.40662204615429	49.35458683372809	8.856118433250726
7	44.515881368281136	3.6293555269459667	48.70277568138552	3.1519874233873812
8	43.48671960891331	11.505668093374037	41.372511640417514	3.6351006572951405
9	40.233670119838195	4.947340657504054	41.57385234492718	13.245136877730568
10	28.787281325976082	15.139985323803382	46.203643979495105	9.869089370725428
11	23.93577988838779	14.3380173555165	49.51283906061896	12.213363695476755
12	17.50462874706541	12.614478250764494	52.00753134360318	17.873361658566903
13	18.71267297412341	14.34637390875166	55.1751873043065	11.765765812818431
14	12.6562610169403	22.374932429432825	50.75326493146321	14.215541622163668
15	10.56712270814999	15.287791859150296	57.36408196734154	16.781003465358168
16	11.918011767071524	15.735912026385817	52.81916157656823	19.526914629974435
17	11.875184431741323	19.421413145380523	53.361815252276514	15.341587170601645
18	12.723896869687387	16.148255700083304	52.403161910830356	18.724685519398953
19	13.587494418083581	16.80894569023824	50.62243264487522	18.981127246802963
20	19.45666735434136	15.563819258199215	53.43180138562098	11.547712001838443
21	14.800761490913555	21.64895686712819	51.40690407982598	12.14337756213228
22	13.377013733472957	14.285788897796742	56.067510504448556	16.26968686428174
23	16.56921706930455	18.25306254618954	52.10310942123034	13.07461096327556
24	14.263591803265847	18.493835736277624	51.37504472061693	15.867527739839607
25	12.665139854752658	23.18891294299525	50.17744618510288	13.968501017149215
26	13.196042127473998	16.097855238383737	55.162391332165164	15.543711301977108
27	18.230343167081447	17.46232369631241	50.74125238618766	13.56608075041848
28	11.6644426048421	20.741748556536	53.54931541549044	14.044493423131463
29	15.839585514959536	15.761242828379899	54.05854287825807	14.34062877840249
30	13.49922832453719	20.73913713365001	52.684934440228446	13.076700101584352
31	15.604296312932028	18.549981328326364	46.96644060449217	18.879281754249437
32	19.44152110160263	21.358566642206338	45.73959413265506	13.460318123535972
33	20.014206140499773	19.490354709570603	43.25952581783236	17.23591333209726
34	17.605951955041743	21.30007076956021	43.09135018397474	18.002627091423303
35	19.330796771236745	20.866052285909024	40.69980910498703	19.103341837867198
36	20.01263928676818	27.571141687971522	37.37755690943324	15.038662115827051
37	15.970417801547528	25.197097142319937	41.499687934965124	17.33279712116741
38	18.110478856614602	25.586721436909325	35.91646580472302	20.386333901753048
39	18.98243295824596	26.892694022191872	37.95703164783396	16.167841371728215
40	18.974337547299395	22.411753492125257	38.668905526554255	19.945003434021093
41	17.30955545748212	24.509248354150724	38.21791279414414	19.963283394223012
42	17.617703358028688	24.23060953221582	38.318974859831876	19.832712249923613
43	21.747929794507133	24.530139737238628	35.36963385239715	18.35229661585708
44	18.09141546954689	23.356305149987072	39.96547698944724	18.586802391018793
45	16.92123687433572	22.547025197619426	38.10744960606686	22.424288321977993
46	20.90522362919884	25.052685456724806	36.96939151235333	17.072699401723014
47	16.197350450339876	24.766212366131935	42.91560142374776	16.12083575978043
48	17.581143437624856	23.85508692121076	39.320194394319635	19.24357524684475
49	16.785965168841546	20.98748345010746	43.59927193529939	18.627279445751608
50	17.58532171424244	21.760203482071276	40.80504944729235	19.849425356393937
51	16.72746929619542	20.86291857844584	39.81479788892574	22.594814236433006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	218.0
2	340.0
3	23517.5
4	46695.0
5	30911.0
6	15127.0
7	14407.5
8	13688.0
9	12966.0
10	12244.0
11	11396.0
12	10548.0
13	9753.5
14	8959.0
15	8090.5
16	7222.0
17	6554.0
18	5886.0
19	5427.0
20	4968.0
21	4553.0
22	4138.0
23	3742.0
24	3346.0
25	3211.0
26	3285.5
27	3495.0
28	3374.5
29	3254.0
30	3624.5
31	3995.0
32	4537.0
33	5079.0
34	5426.5
35	5774.0
36	6104.0
37	6434.0
38	7162.5
39	7891.0
40	8643.5
41	9396.0
42	10664.5
43	11933.0
44	13121.5
45	14310.0
46	25493.0
47	36676.0
48	29514.5
49	22353.0
50	22439.5
51	22526.0
52	20041.5
53	17557.0
54	16757.5
55	15958.0
56	15207.0
57	14456.0
58	13044.0
59	11632.0
60	11091.5
61	10551.0
62	9416.5
63	8282.0
64	7133.5
65	5985.0
66	4538.5
67	3092.0
68	2647.5
69	2203.0
70	1848.5
71	1494.0
72	1211.5
73	929.0
74	775.0
75	447.5
76	274.0
77	284.5
78	295.0
79	189.0
80	83.0
81	64.5
82	46.0
83	31.5
84	17.0
85	9.5
86	2.0
87	3.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.239384935735856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72242892275106	32.482208903701576
2	7.093317785317543	5.70861489669788
3	2.600827612558704	3.139671103597217
4	1.488388463266278	2.3956734522912044
5	1.029133655525956	2.0705852657514967
6	0.8601183242463464	2.076637940377728
7	0.6909132921783026	1.9461348144825479
8	0.6369922120572612	2.0505739857630414
9	0.5665355169005297	2.051733666989385
>10	4.15664576819343	26.89998186916699
>50	0.11077982010074175	3.0407397579140354
>100	0.03539710228071038	2.6239832678460115
>500	0.003933011364523375	1.1183845049926708
>1k	0.002622007576348917	2.302183952730196
>5k	0.0013110037881744585	2.808094679281126
>10k+	6.555018940872292E-4	7.284797938416883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27618	7.21222772652135	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGC	5596	1.4613522469988223	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC	5050	1.3187685574238837	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCG	4960	1.2952657514499926	No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	1442	0.37656718015945345	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTAAGTCGT	1318	0.3441855363732037	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCT	1008	0.2632314269075791	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTA	855	0.22327665675196443	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGTCGAATGC	808	0.21100296918782138	No Hit
GAACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCT	758	0.19794585475788196	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTC	680	0.1775767562471764	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	574	0.14989567365570478	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	565	0.14754539305831568	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	485	0.1266540099704126	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	434	0.11333575325187435	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	384	0.10027863882193491	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.222845771975776E-4	0.0	0.0	0.08696038210339668	0.0
2	5.222845771975776E-4	0.0	0.0	0.6512888677653793	0.0
3	5.222845771975776E-4	0.0	0.0	0.8447953036170819	0.0
4	5.222845771975776E-4	0.0	0.0	1.240164728555648	0.0
5	5.222845771975776E-4	0.0	0.0	3.145458866172411	0.0
6	5.222845771975776E-4	0.0	0.0	3.5358665876276008	0.0
7	5.222845771975776E-4	0.0	0.0	4.096800223537799	0.0
8	5.222845771975776E-4	0.0	0.0	4.700038910201001	0.0
9	5.222845771975776E-4	0.0	0.0	4.863252840575244	0.0
10	5.222845771975776E-4	0.0	0.0	6.595409640851011	0.0
11	5.222845771975776E-4	0.0	0.0	7.187680351393063	0.0
12	5.222845771975776E-4	0.0	0.0	9.228246194503999	0.0
13	5.222845771975776E-4	0.0	0.0	9.507668443304704	0.0
14	5.222845771975776E-4	0.0	0.0	9.65286355576563	0.0
15	5.222845771975776E-4	0.0	0.0	10.059462099113944	0.0
16	5.222845771975776E-4	0.0	0.0	10.425844730018046	0.0
17	5.222845771975776E-4	0.0	0.0	10.80632904450648	0.0
18	5.222845771975776E-4	0.0	0.0	11.173756244564975	0.0
19	7.834268657963665E-4	0.0	0.0	11.805720582974045	0.0
20	7.834268657963665E-4	0.0	0.0	12.11517419496361	0.0
21	7.834268657963665E-4	0.0	0.0	12.404519850731068	0.0
22	7.834268657963665E-4	0.0	0.0	12.785004165219503	0.0
23	7.834268657963665E-4	0.0	0.0	13.080094951336134	0.0
24	7.834268657963665E-4	0.0	0.0	13.326613271773391	0.0
25	7.834268657963665E-4	0.0	0.0	13.537093956384014	0.0
26	7.834268657963665E-4	0.0	0.0	13.738173518605082	0.0
27	7.834268657963665E-4	0.0	0.0	14.037964865916491	0.0
28	7.834268657963665E-4	0.0	0.0	14.237216432117368	0.0
29	0.001305711442993944	0.0	0.0	14.456837096828949	0.0
30	0.001305711442993944	0.0	0.0	14.711450828212769	0.0
31	0.001305711442993944	0.0	0.0	14.956141152629833	0.0
32	0.001566853731592733	0.0	0.0	15.186990935751163	0.0
33	0.0018279960201915218	0.0	0.0	15.401127612402169	0.0
34	0.0018279960201915218	0.0	0.0	15.624143126865535	0.0
35	0.0018279960201915218	0.0	0.0	15.842719222422721	0.0
36	0.0018279960201915218	0.0	0.0	16.08610383539679	0.0
37	0.0018279960201915218	0.0	0.0	16.32870502150507	0.0
38	0.0018279960201915218	0.0	0.0	16.55041482452544	0.0
39	0.0018279960201915218	0.0	0.0	16.798761140982887	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACGG	45	3.8380676E-10	45.000004	2
ATCGAAT	35	1.2090823E-7	45.000004	43
AAATCGT	40	6.7939254E-9	45.0	45
CCTAGTC	20	7.0273876E-4	45.0	29
ACTGACG	50	2.1827873E-11	45.0	43
CTATCGG	20	7.0273876E-4	45.0	18
ACCGCCG	20	7.0273876E-4	45.0	39
CTATACG	20	7.0273876E-4	45.0	32
CGACAAT	25	3.885917E-5	45.0	20
ACGGGTA	25	3.885917E-5	45.0	5
CGTTAGG	25	3.885917E-5	45.0	2
ATCCGGG	25	3.885917E-5	45.0	3
ATCCGGC	50	2.1827873E-11	45.0	6
GCCGATC	20	7.0273876E-4	45.0	9
TCATCGA	25	3.885917E-5	45.0	16
CTCGAGG	25	3.885917E-5	45.0	2
CATCGAA	40	6.7939254E-9	45.0	42
ATACGTA	20	7.0273876E-4	45.0	34
CACCGCG	25	3.885917E-5	45.0	38
TATACGT	20	7.0273876E-4	45.0	33
>>END_MODULE