##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515866
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.514914725917194	34.0	31.0	34.0	31.0	34.0
2	32.717100952572956	34.0	31.0	34.0	31.0	34.0
3	32.558836984798376	34.0	31.0	34.0	31.0	34.0
4	36.13881318016694	37.0	37.0	37.0	35.0	37.0
5	36.16420543319389	37.0	37.0	37.0	35.0	37.0
6	36.17497179500103	37.0	37.0	37.0	35.0	37.0
7	36.29711979467536	37.0	37.0	37.0	35.0	37.0
8	36.07675442847561	37.0	37.0	37.0	35.0	37.0
9	37.887145111327364	39.0	38.0	39.0	35.0	39.0
10	37.759786456172726	39.0	38.0	39.0	35.0	39.0
11	37.73469466877057	39.0	38.0	39.0	35.0	39.0
12	37.67983352265899	39.0	38.0	39.0	35.0	39.0
13	37.689820612329555	39.0	38.0	39.0	35.0	39.0
14	39.143831925344955	41.0	39.0	41.0	35.0	41.0
15	39.22605676667972	41.0	39.0	41.0	35.0	41.0
16	39.26217661175576	41.0	39.0	41.0	35.0	41.0
17	39.22114269984841	41.0	39.0	41.0	35.0	41.0
18	38.534200742053166	39.0	39.0	41.0	35.0	41.0
19	37.80321440063893	37.0	37.0	41.0	35.0	41.0
20	36.95379614085829	36.0	35.0	41.0	34.0	41.0
21	36.90988938988032	36.0	35.0	40.0	34.0	41.0
22	36.89972008234697	35.0	35.0	40.0	34.0	41.0
23	36.90785397758333	35.0	35.0	40.0	34.0	41.0
24	36.73878875521938	35.0	35.0	40.0	34.0	41.0
25	36.651950312678096	35.0	35.0	40.0	34.0	41.0
26	36.493240492686084	35.0	35.0	40.0	33.0	41.0
27	36.405188556718215	35.0	35.0	40.0	33.0	41.0
28	36.24659310751242	35.0	35.0	40.0	33.0	41.0
29	35.966551391252764	35.0	35.0	40.0	33.0	41.0
30	35.4268337126308	35.0	35.0	40.0	31.0	41.0
31	34.73189161526443	35.0	35.0	40.0	23.0	41.0
32	33.61133899113336	35.0	33.0	40.0	17.0	41.0
33	32.38140524864984	35.0	32.0	40.0	12.0	41.0
34	31.37153059127758	35.0	27.0	40.0	8.0	41.0
35	30.75948405206003	35.0	23.0	40.0	7.0	41.0
36	30.40630318726181	35.0	22.0	40.0	7.0	41.0
37	30.197911860832075	35.0	21.0	40.0	7.0	41.0
38	30.020187413010355	35.0	21.0	40.0	7.0	41.0
39	29.884700678083068	35.0	20.0	40.0	7.0	41.0
40	29.71836290819709	35.0	18.0	40.0	7.0	41.0
41	29.55268616268566	35.0	18.0	40.0	7.0	41.0
42	29.415171769412986	35.0	18.0	40.0	7.0	41.0
43	29.301640348462584	35.0	18.0	40.0	7.0	41.0
44	29.214454916586867	35.0	18.0	40.0	7.0	41.0
45	29.15097331477554	35.0	18.0	40.0	7.0	41.0
46	29.097986686465088	35.0	18.0	40.0	7.0	41.0
47	29.112614516172805	35.0	20.0	40.0	7.0	41.0
48	29.075622351540904	35.0	20.0	40.0	7.0	41.0
49	29.051639379218635	35.0	20.0	40.0	7.0	41.0
50	28.95489526349866	35.0	20.0	39.0	7.0	41.0
51	28.33040169346303	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	11.0
11	12.0
12	5.0
13	13.0
14	20.0
15	34.0
16	82.0
17	122.0
18	301.0
19	530.0
20	877.0
21	1457.0
22	2290.0
23	3835.0
24	7360.0
25	15356.0
26	27502.0
27	35031.0
28	32353.0
29	24910.0
30	18334.0
31	14949.0
32	13562.0
33	14329.0
34	22867.0
35	33014.0
36	34251.0
37	33755.0
38	60112.0
39	118455.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.131398463942183	12.733733178771232	13.607409676156212	47.52745868113037
2	60.17240911399472	14.46926139733962	14.499114111028833	10.85921537763683
3	20.238976788545862	14.958535743778423	54.18810311204848	10.614384355627235
4	18.150837620622408	15.720943035594514	53.887637487254445	12.240581856528634
5	15.86012646695072	18.803138799610753	52.74160343965293	12.595131293785593
6	16.426746480675213	21.66531618676168	52.59776763733217	9.310169695230933
7	49.49987012131057	3.9628508178480457	43.35370037955593	3.1835786812854505
8	49.60803774623643	3.8705787937177485	43.31008440176325	3.2112990582825773
9	46.038506123683284	5.178476581127657	44.5551751811517	4.227842114037366
10	25.39205917815867	16.639980149883886	48.73668743433372	9.231273237623725
11	19.337579914163754	16.211574323564644	52.3864724560254	12.064373306246196
12	18.87079202738696	14.00363660330396	55.130789778741	11.994781590568094
13	16.253057964665242	14.466547514276964	55.52410897403589	13.756285547021902
14	12.944059116127058	17.027290032682906	55.61347326631334	14.415177584876693
15	12.527865763589768	16.50002132336692	57.8371127385794	13.135000174463912
16	14.40974981875138	15.367168993498312	57.461433783191765	12.761647404558548
17	14.233735117259133	15.242330372616145	55.53670914539822	14.987225364726498
18	14.822647741855445	16.409687787138523	54.85455525272067	13.913109218285369
19	15.993106737020854	17.36827005462659	53.8901575215269	12.74846568682565
20	17.760426157180355	15.603470668739556	54.44514660784002	12.19095656624007
21	16.926488661784262	16.10573288412107	54.116960606048856	12.850817848045809
22	15.051001616698912	15.58253499939907	54.67214354115216	14.694319842749861
23	15.452656309971967	16.30714177712817	54.794462127761854	13.445739785138
24	15.166341646861781	15.290986418953759	55.33820798424397	14.204463949940488
25	15.17487099363013	16.254414906196573	53.88550515056235	14.685208949610946
26	13.983282480334038	17.320001705869352	54.269713452718335	14.427002361078264
27	15.085700550142867	16.84274598442231	54.60894883555032	13.462604629884506
28	13.628151496706508	17.74957062492973	54.62213055328322	14.000147325080544
29	14.704981526210295	17.391144211868973	53.91438862030063	13.989485641620112
30	15.299903463302487	16.731282930063234	53.187261808299056	14.781551798335228
31	15.488712184947254	18.606576126358394	50.7189851628136	15.185726525880753
32	16.278452156180094	20.21532723614272	49.3676652464012	14.138555361275989
33	16.39534297666448	21.734714053649594	46.00652107330198	15.863421896383944
34	16.374795004904374	23.57957298988497	43.843556272365305	16.20207573284535
35	16.973400069010168	24.52458584205976	41.18550166128413	17.31651242764594
36	17.46364365939992	26.87306393520798	38.66100886664366	17.002283538748433
37	18.479217471203764	26.839528094505162	38.9068091326042	15.774445301686873
38	17.415569159432877	26.45202436291595	38.430522655108106	17.701883822543063
39	18.445293932920563	25.14509581945699	38.479178701445726	17.93043154617672
40	18.595720594107775	25.078993382002302	39.51394354347835	16.81134248041158
41	16.69464550871738	27.031244548002775	38.30064396568101	17.973465977598835
42	17.207181709978947	27.43638851949925	37.63806880081261	17.718360969709188
43	18.183791914954657	26.044360357147013	37.894336901443396	17.87751082645493
44	17.79357430030279	25.785572222243765	37.996495213873374	18.424358263580075
45	17.878092372825503	24.501323987236994	38.43730736276475	19.18327627717275
46	17.243431433744423	25.257140420186637	39.04521716879965	18.45421097726929
47	17.180236728142578	24.737044116107672	40.66210992777194	17.420609227977806
48	16.66925131720253	23.681925151105133	41.01762860897985	18.63119492271249
49	16.29415390818546	23.66874343337223	41.58560556423567	18.451497094206633
50	15.623243245338905	23.01082063946839	41.90002830192337	19.465907813269336
51	15.594165926810453	22.95053366571939	41.15894437702814	20.296356030442013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	307.0
2	486.0
3	33340.5
4	66195.0
5	44620.0
6	23045.0
7	21915.0
8	20785.0
9	19399.5
10	18014.0
11	16932.5
12	15851.0
13	14723.5
14	13596.0
15	12495.0
16	11394.0
17	10432.5
18	9471.0
19	8703.5
20	7936.0
21	7027.5
22	6119.0
23	5516.5
24	4914.0
25	4651.0
26	4467.0
27	4546.0
28	4708.5
29	4871.0
30	5217.5
31	5564.0
32	6362.5
33	7161.0
34	7784.0
35	8407.0
36	9178.0
37	9949.0
38	10334.0
39	10719.0
40	12298.0
41	13877.0
42	15034.5
43	16192.0
44	17301.0
45	18410.0
46	20741.0
47	23072.0
48	25226.5
49	27381.0
50	27269.0
51	27157.0
52	24635.5
53	22114.0
54	20572.5
55	19031.0
56	18445.0
57	17859.0
58	17416.0
59	16973.0
60	17235.0
61	17497.0
62	16003.5
63	14510.0
64	12505.0
65	10500.0
66	8576.5
67	6653.0
68	5394.5
69	4136.0
70	3575.0
71	3014.0
72	2371.5
73	1729.0
74	1385.5
75	829.5
76	617.0
77	466.5
78	316.0
79	236.0
80	156.0
81	108.0
82	60.0
83	37.5
84	15.0
85	12.5
86	10.0
87	6.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	515866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.64753954387736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24748597616767	33.79710291921477
2	7.096668951615448	6.053109394876531
3	2.781876503992414	3.5592056463059936
4	1.7745147974312439	3.0271475997857804
5	1.3132251772726877	2.8002911338876157
6	1.1119453695758919	2.8453040471771507
7	0.9110420333789445	2.7197590801264084
8	0.8135201286531214	2.7755705485179356
9	0.6954459238140228	2.669315178283817
>10	4.11962490745016	26.22706531053262
>50	0.08518688061449062	2.6298310867708814
>100	0.046715386143195856	3.313071516164931
>500	0.002289969908980189	0.6142913523369125
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.5799398179603777E-4	6.968935186018664
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35679	6.916330985178322	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	772	0.14965126602644874	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	669	0.12968484063690958	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	635	0.12309398177045977	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	559	0.10836147371604254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.815463705691013E-4	0.0	0.0	0.05912388100785863	0.0
2	5.815463705691013E-4	0.0	0.0	0.29581325382948287	0.0
3	5.815463705691013E-4	0.0	0.0	0.4491476468695359	0.0
4	5.815463705691013E-4	0.0	0.0	0.6395071588358217	0.0
5	5.815463705691013E-4	0.0	0.0	1.194496245148934	0.0
6	5.815463705691013E-4	0.0	0.0	1.5391593941062214	0.0
7	5.815463705691013E-4	0.0	0.0	1.8233417205243223	0.0
8	5.815463705691013E-4	0.0	0.0	2.295169675846053	0.0
9	5.815463705691013E-4	0.0	0.0	2.494058534580686	0.0
10	5.815463705691013E-4	0.0	0.0	2.907344155265127	0.0
11	5.815463705691013E-4	0.0	0.0	3.447019187153253	0.0
12	5.815463705691013E-4	0.0	0.0	3.9171025033632767	0.0
13	5.815463705691013E-4	0.0	0.0	4.085169404457747	0.0
14	5.815463705691013E-4	0.0	0.0	4.16677974512761	0.0
15	5.815463705691013E-4	0.0	0.0	4.318757196636335	0.0
16	5.815463705691013E-4	0.0	0.0	4.619416670220561	0.0
17	5.815463705691013E-4	0.0	0.0	4.949153462333242	0.0
18	5.815463705691013E-4	0.0	0.0	5.294398157661098	0.0
19	5.815463705691013E-4	0.0	0.0	5.516936568798874	0.0
20	9.692439509485022E-4	0.0	0.0	5.733853365021149	0.0
21	9.692439509485022E-4	0.0	0.0	6.016678749907922	0.0
22	9.692439509485022E-4	0.0	0.0	6.322959838407648	0.0
23	9.692439509485022E-4	0.0	0.0	6.6069483160355595	0.0
24	9.692439509485022E-4	0.0	0.0	6.841893049745477	0.0
25	9.692439509485022E-4	0.0	0.0	7.04698506976618	0.0
26	9.692439509485022E-4	0.0	0.0	7.23443684987962	0.0
27	9.692439509485022E-4	0.0	0.0	7.422470176363629	0.0
28	9.692439509485022E-4	0.0	0.0	7.6242667669511075	0.0
29	9.692439509485022E-4	0.0	0.0	7.832460367614845	0.0
30	9.692439509485022E-4	0.0	0.0	8.080199121477284	0.0
31	9.692439509485022E-4	0.0	0.0	8.310491484222647	0.0
32	9.692439509485022E-4	0.0	0.0	8.523919002221508	0.0
33	9.692439509485022E-4	0.0	0.0	8.744325076667197	0.0
34	9.692439509485022E-4	0.0	0.0	8.953100223701504	0.0
35	9.692439509485022E-4	0.0	0.0	9.194054269907301	0.0
36	9.692439509485022E-4	0.0	0.0	9.44159917497955	0.0
37	9.692439509485022E-4	0.0	0.0	9.694571846177109	0.0
38	9.692439509485022E-4	0.0	0.0	9.940178263347459	0.0
39	0.0011630927411382026	0.0	0.0	10.180356914392497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	90	0.0	45.000004	5
GTTAGCG	40	6.8030204E-9	45.000004	1
CACGACG	20	7.0296664E-4	45.000004	26
TACGGGT	20	7.0296664E-4	45.000004	4
TACTAGG	20	7.0296664E-4	45.000004	2
CTACGAA	55	1.8189894E-12	45.0	11
TACGAAT	55	1.8189894E-12	45.0	12
TCGAGCG	25	3.887806E-5	45.0	32
GCTACGA	55	1.8189894E-12	45.0	10
CGTTTTT	20295	0.0	44.456764	1
GGCGATA	85	0.0	42.352943	8
ATGGGTA	80	0.0	42.187504	5
CGAATAT	60	3.6379788E-12	41.250004	14
GTTTTTT	22375	0.0	40.43464	2
GCGATGA	45	1.9246727E-8	40.000004	9
ATTGCGG	40	3.4532786E-7	39.375004	2
AATCGCG	35	6.2415947E-6	38.571426	1
AATGGGT	65	9.094947E-12	38.07692	4
ACCTATG	65	9.094947E-12	38.07692	34
GCGGGAT	95	0.0	37.894737	5
>>END_MODULE