FastQCFastQC Report
Sat 18 Jun 2016
SRR3550816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541436
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301115.561322113786302No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC9320.1721348414217008TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC9180.1695491249196581TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6790.1254072503490717No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT6310.11654193662778242TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG6290.11617254855606203No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5780.10675315272719213No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5730.10582968254789116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG302.163366E-645.0000042
TTGCGAG207.029975E-445.0000041
TGTGTCG207.029975E-445.0000041
CTGGCGA207.029975E-445.00000414
CGCGGGT253.888063E-545.04
TAGTTCG351.2103374E-745.01
CGTTTTT199700.044.639461
ACGGGCG556.002665E-1140.909095
TAGGGCG1100.040.909095
GTTTTTT222350.040.1023182
CGGGTGA451.9250365E-840.06
TCGTTGG403.453697E-739.3750042
TACGGGT403.453697E-739.3750044
AGACACG403.453697E-739.37500424
GCGAAAG356.2421695E-638.5714261
TACTAGG356.2421695E-638.5714262
TTACGGG659.094947E-1238.076923
CCCGCAC1250.037.833
CGGGTCA301.1391243E-437.5000046
TGCGTAG601.546141E-1037.5000041