SRR3550815_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284062
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19281	6.787602706451408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	4157	1.4634129168984236	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	3740	1.3166139786384663	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	3484	1.2264928079081328	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1308	0.4604628567002978	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	985	0.34675528581788484	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	840	0.2957100914589068	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	580	0.20418077743591187	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	568	0.1999563475579275	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	527	0.1855228788081475	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	481	0.1693292309425407	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	431	0.15172743978427244	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	353	0.12426864557737395	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	343	0.1207482873457203	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	340	0.1196921798762242	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	294	0.1034985320106174	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGGC	30	2.1597116E-6	45.000004	6
AAACGAT	30	2.1597116E-6	45.000004	16
TCTCGAA	60	0.0	45.000004	42
AATTATC	60	0.0	45.000004	45
CTCTCGA	60	0.0	45.000004	41
AACAAGC	45	3.8198777E-10	45.0	13
CTACTGG	25	3.8833678E-5	45.0	2
ACTGACG	20	7.024311E-4	45.0	43
GCGAACC	20	7.024311E-4	45.0	33
CTATATC	20	7.024311E-4	45.0	19
CGTCTAC	20	7.024311E-4	45.0	42
AATGGGC	35	1.2076089E-7	45.0	4
AGGGTGT	20	7.024311E-4	45.0	6
CCTCGAA	40	6.7830115E-9	45.0	42
CCGTCTA	25	3.8833678E-5	45.0	41
CACCTCG	25	3.8833678E-5	45.0	38
GTTACGG	20	7.024311E-4	45.0	2
TACGCGG	20	7.024311E-4	45.0	2
TAACCAG	20	7.024311E-4	45.0	1
TGCGAAG	20	7.024311E-4	45.0	1