FastQCFastQC Report
Sat 18 Jun 2016
SRR3550815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284062
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192816.787602706451408No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC41571.4634129168984236No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG37401.3166139786384663No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC34841.2264928079081328TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC13080.4604628567002978TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT9850.34675528581788484No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT8400.2957100914589068No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC5800.20418077743591187TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA5680.1999563475579275No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC5270.1855228788081475No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT4810.1693292309425407Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4310.15172743978427244No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3530.12426864557737395No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3430.1207482873457203No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT3400.1196921798762242Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA2940.1034985320106174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGC302.1597116E-645.0000046
AAACGAT302.1597116E-645.00000416
TCTCGAA600.045.00000442
AATTATC600.045.00000445
CTCTCGA600.045.00000441
AACAAGC453.8198777E-1045.013
CTACTGG253.8833678E-545.02
ACTGACG207.024311E-445.043
GCGAACC207.024311E-445.033
CTATATC207.024311E-445.019
CGTCTAC207.024311E-445.042
AATGGGC351.2076089E-745.04
AGGGTGT207.024311E-445.06
CCTCGAA406.7830115E-945.042
CCGTCTA253.8833678E-545.041
CACCTCG253.8833678E-545.038
GTTACGG207.024311E-445.02
TACGCGG207.024311E-445.02
TAACCAG207.024311E-445.01
TGCGAAG207.024311E-445.01