FastQCFastQC Report
Sat 18 Jun 2016
SRR3550814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394607
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291917.397486613263323No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC11660.2954838611580636No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC11530.29218944418117265No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC11080.28078569310731943No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC10660.2701421921050564No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG9870.2501222735531808No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT6840.17333701632256904Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6070.15382393115175352No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4530.11479776081012248No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG4270.10820892685634062No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA4200.10643501002263012No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA4020.10187350959308883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAC253.8861333E-545.00000441
TAGCTAG253.8861333E-545.0000041
CGCATAT207.027651E-445.026
ATTACGG207.027651E-445.02
TCGATGG207.027651E-445.02
GCTTGAC207.027651E-445.043
TATTGCG207.027651E-445.01
GAGCGTC207.027651E-445.015
CGCTAAG207.027651E-445.03
TCGCTAA207.027651E-445.02
TCATCGC207.027651E-445.022
CATCGCA207.027651E-445.023
ATGCGAG207.027651E-445.01
GCTCATG207.027651E-445.01
GAGTAAG302.1618653E-644.9999961
GGCCGAT302.1618653E-644.9999968
ATAGCGG302.1618653E-644.9999962
CGTTTTT144900.044.6739161
TAGGGCG750.042.05
ACGGGCT700.041.7857175