Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550802_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 710422 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 35 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 51471 | 7.2451303591386536 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA | 1074 | 0.15117775068902709 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA | 1012 | 0.1424505434797909 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA | 897 | 0.12626298172072373 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA | 832 | 0.11711349029168579 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC | 715 | 0.10064440571941748 | Illumina PCR Primer Index 7 (96% over 27bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATAGGCG | 20 | 7.031465E-4 | 45.0 | 1 |
GACCGAC | 20 | 7.031465E-4 | 45.0 | 9 |
TAACGAT | 25 | 3.889296E-5 | 45.0 | 17 |
ATCGTTG | 50 | 2.1827873E-11 | 45.0 | 23 |
ATAACGA | 25 | 3.889296E-5 | 45.0 | 16 |
CGTTTTT | 27465 | 0.0 | 44.58219 | 1 |
TTACACG | 70 | 0.0 | 41.785717 | 34 |
GTTTTTT | 30045 | 0.0 | 40.963554 | 2 |
AATCGTT | 55 | 6.002665E-11 | 40.909092 | 22 |
ACAACGA | 45 | 1.9264917E-8 | 40.0 | 13 |
CTACGAA | 130 | 0.0 | 39.80769 | 11 |
TAGCCGT | 85 | 0.0 | 39.705883 | 44 |
CGATGCG | 40 | 3.4557343E-7 | 39.375 | 10 |
CGAGATA | 95 | 0.0 | 37.894737 | 19 |
GGCCGAT | 60 | 1.546141E-10 | 37.499996 | 8 |
ACGATTC | 30 | 1.1394832E-4 | 37.499996 | 19 |
CACTCGT | 30 | 1.1394832E-4 | 37.499996 | 22 |
GTGCTAA | 30 | 1.1394832E-4 | 37.499996 | 25 |
TCGTAAG | 30 | 1.1394832E-4 | 37.499996 | 1 |
CGGTCTA | 30 | 1.1394832E-4 | 37.499996 | 31 |