FastQCFastQC Report
Sat 18 Jun 2016
SRR3550802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710422
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514717.2451303591386536No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10740.15117775068902709No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10120.1424505434797909No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA8970.12626298172072373No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA8320.11711349029168579No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC7150.10064440571941748Illumina PCR Primer Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG207.031465E-445.01
GACCGAC207.031465E-445.09
TAACGAT253.889296E-545.017
ATCGTTG502.1827873E-1145.023
ATAACGA253.889296E-545.016
CGTTTTT274650.044.582191
TTACACG700.041.78571734
GTTTTTT300450.040.9635542
AATCGTT556.002665E-1140.90909222
ACAACGA451.9264917E-840.013
CTACGAA1300.039.8076911
TAGCCGT850.039.70588344
CGATGCG403.4557343E-739.37510
CGAGATA950.037.89473719
GGCCGAT601.546141E-1037.4999968
ACGATTC301.1394832E-437.49999619
CACTCGT301.1394832E-437.49999622
GTGCTAA301.1394832E-437.49999625
TCGTAAG301.1394832E-437.4999961
CGGTCTA301.1394832E-437.49999631