SRR3550801_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	4733	1.570849181220171	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	4207	1.3962735063159224	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	3555	1.1798793237349903	No Hit
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1730	0.5741747482592217	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	917	0.30434580586919435	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	840	0.27879005117788797	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGT	823	0.27314787157071646	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	703	0.23332072140244672	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.22900611346755081	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTA	652	0.2163941825809321	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC	646	0.2144028250725186	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	558	0.18519624828245415	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	515	0.17092485280549083	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	325	0.10786519837239714	TruSeq Adapter, Index 19 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	325	0.10786519837239714	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	322	0.10686951961819038	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	314	0.10421437627363907	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	308	0.10222301876522558	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACC	50	2.1827873E-11	45.000004	7
TCGGGTT	25	3.8839316E-5	45.000004	21
CGTAGTG	25	3.8839316E-5	45.000004	2
TATAGCG	25	3.8839316E-5	45.000004	1
TTCGGGA	35	1.2079363E-7	45.000004	4
GTAAGCG	25	3.8839316E-5	45.000004	1
CACAACG	35	1.2079363E-7	45.000004	12
GCGTAGT	25	3.8839316E-5	45.000004	1
TCCGGCC	20	7.0249935E-4	45.0	23
TATCACT	20	7.0249935E-4	45.0	30
CAGCGCA	20	7.0249935E-4	45.0	20
CGGCGAA	20	7.0249935E-4	45.0	31
CTGTACG	20	7.0249935E-4	45.0	1
ACGCATG	20	7.0249935E-4	45.0	1
GCGAATG	20	7.0249935E-4	45.0	1
ACACCGT	20	7.0249935E-4	45.0	38
CGGCATT	20	7.0249935E-4	45.0	24
CGGCAGA	20	7.0249935E-4	45.0	31
CCCACGA	45	3.8198777E-10	45.0	39
TCGGACA	20	7.0249935E-4	45.0	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content